Recombinant Human RCL protein is a Human Full Length protein, in the 1 to 174 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A A A M V P G R S E S W E R G E P G R P A L Y F C G S I R G G R E D R T L Y E R I V S R L R R F G T V L T E H V A A A E L G A R G E E A A G G D R L I H E Q D L E W L Q Q A D V V V A E V T Q P S L G V G Y E L G R A V A F N K R I L C L F R P Q S G R V L S A M I R G A A D G S R F Q V W D Y E E G E V E A L L D R Y F E A D P P G Q V A A S P D P T T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Part of a nucleotide salvage pathway that eliminates epigenetically modified 5-hydroxymethyl-dCMP (hmdCMP) in a two-step process entailing deamination to cytotoxic 5-hydroxymethyl-dUMP (hmdUMP), followed by its hydrolysis into 5-hydroxymethyluracil (hmU) and 2-deoxy-D-ribose 5-phosphate (deoxyribosephosphate) (PubMed:33833118). Catalyzes the second step in that pathway, the hydrolysis of the N-glycosidic bond in hmdUMP, degrading this cytotoxic nucleotide to avoid its genomic integration (PubMed:33833118).
Alternative names
C6orf108, RCL, DNPH1, 5-hydroxymethyl-dUMP N-hydrolase, 2'-deoxynucleoside 5'-phosphate N-hydrolase 1, c-Myc-responsive protein RCL
Recommended products
Recombinant Human RCL protein is a Human Full Length protein, in the 1 to 174 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- O43598-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A A A M V P G R S E S W E R G E P G R P A L Y F C G S I R G G R E D R T L Y E R I V S R L R R F G T V L T E H V A A A E L G A R G E E A A G G D R L I H E Q D L E W L Q Q A D V V V A E V T Q P S L G V G Y E L G R A V A F N K R I L C L F R P Q S G R V L S A M I R G A A D G S R F Q V W D Y E E G E V E A L L D R Y F E A D P P G Q V A A S P D P T T
- Accession
- O43598
- Protein length
- Full Length
- Predicted molecular weight
- 21.2 kDa
- Amino acids
- 1 to 174
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab100943 was purified using conventional chromatography techniques.
General info
- Function
Part of a nucleotide salvage pathway that eliminates epigenetically modified 5-hydroxymethyl-dCMP (hmdCMP) in a two-step process entailing deamination to cytotoxic 5-hydroxymethyl-dUMP (hmdUMP), followed by its hydrolysis into 5-hydroxymethyluracil (hmU) and 2-deoxy-D-ribose 5-phosphate (deoxyribosephosphate) (PubMed:33833118). Catalyzes the second step in that pathway, the hydrolysis of the N-glycosidic bond in hmdUMP, degrading this cytotoxic nucleotide to avoid its genomic integration (PubMed:33833118).
- Sequence similarities
Belongs to the 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The reactive center loop (RCL) is an important structural element found in serpins including alpha-1 antitrypsin. It functions by acting as a bait for target proteases and then trapping them modifying their activity in proteolytic cascade reactions. The RCL's standard molecular mass is around 10 kDa but it is most often part of larger serpin proteins. RCLs are found in a variety of tissues prominently in the liver where they play a role in regulating enzymes in the blood.
- Biological function summary
The reactive center loop operates by temporarily interacting with proteases to regulate their activities. It often finds itself as a component of a serpin-protease complex where it changes the enzymatic activity. This precise control allows proper regulation of proteolytic pathways that manage diverse physiological processes including inflammation and coagulation.
- Pathways
Reactive center loops engage in the regulation of two main pathways: the coagulation cascade and the inflammatory response pathway. In these pathways RCLs work closely with proteins like thrombin and plasminogen to maintain a balance between enzyme activation and inhibition. Through these interactions RCLs help modulate the clot formation and dissolution as well as inflammatory response to injury.
- Associated diseases and disorders
Reactive center loops have ties to alpha-1 antitrypsin deficiency and emphysema. Aberrant RCL function can lead to inefficient enzyme inhibition contributing to these conditions. The protein alpha-1 antitrypsin which includes the RCL when mutated or deficient can result in unregulated neutrophil elastase activity causing tissue damage and related respiratory conditions.
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1 product image
SDS-PAGE - Recombinant Human RCL protein (ab100943)
15% SDS-PAGE showing ab100943 at approximately 21.2kDa (3µg).
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