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AB105594

Recombinant Human RCN2 protein

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(1 Publication)

Recombinant Human RCN2 protein is a Human Full Length protein, in the 26 to 317 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

ERC55, RCN2, Reticulocalbin-2, Calcium-binding protein ERC-55, E6-binding protein, E6BP

1 Images
SDS-PAGE - Recombinant Human RCN2 protein (AB105594)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RCN2 protein (AB105594)

15% SDS-PAGEanalysis of 3μg ab105594.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q14257

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMEELHYPLGERRSDYDREALLGVQEDVDEYVKLGHEEQQKRLQAIIKKIDLDSDGFLTESELSSWIQMSFKHYAMQEAKQQFVEYDKNSDDTVTWDEYNIQMYDRVIDFDENTALDDAEEESFRKLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLEEFLGDYRWDPTANEDPEWILVEKDRFVNDYDKDNDGRLDPQELLPWVVPNNQGIAQEEALHLIDEMDLNGDKKLSEEEILENPDLFLTSEATDYGRQLHDDYFYHDEL","proteinLength":"Full Length","predictedMolecularWeight":"36.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":317,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q14257","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Reticulocalbin 2 (RCN2) also known as E6-binding protein and ERC-55 is a calcium-binding protein with a mass of approximately 39 kDa. It localizes primarily to the endoplasmic reticulum where it helps in protein folding and processing. RCN2 is broadly expressed in many tissue types notably in the brain heart and liver. Functionally RCN2 contains EF-hand motifs that bind calcium ions which is important for its role in maintaining calcium homeostasis within cells.
Biological function summary

This protein interacts with cellular mechanisms associated with calcium signaling pathways. RCN2 forms part of a larger molecular complex that influences protein processing in the endoplasmic reticulum. As a chaperone it helps in ensuring that proteins achieve their correct conformations. Research shows that RCN2 also interacts with various cell signaling molecules and contributes to the regulation of cellular stress responses.

Pathways

Involvement of RCN2 extends to the unfolded protein response (UPR) and calcium signaling pathways. In the UPR RCN2 helps maintain endoplasmic reticulum function and protein folding. RCN2 interacts with significant pathway proteins such as GRP78/BiP which is an important regulator in maintaining protein-folding homeostasis. In calcium signaling the regulation by RCN2 supports intracellular calcium balance and communication between organelles.

Researchers have linked RCN2 to conditions such as neurodegenerative disorders and cardiovascular diseases. Altered expression of RCN2 has been noted in Alzheimer’s disease potentially impacting calcium signaling and protein misfolding. Additionally RCN2 interacts with proteins involved in heart conditions such as SERCA2 affecting calcium uptake in cardiac cells. Understanding RCN2’s roles may provide insights into therapeutic approaches for these disorders.

Specifications

Form

Liquid

Additional notes

ab105594 was purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

General info

Function

Not known. Binds calcium.

Sequence similarities

Belongs to the CREC family.

Product protocols

Target data

Not known. Binds calcium.
See full target information RCN2

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell research 35:165-185 PubMed39875551

2025

Raptin, a sleep-induced hypothalamic hormone, suppresses appetite and obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Qi Xie,Biao Hu,Ren-Bin Lu,Ya-Lun Cheng,Xin Chen,Jie Wen,Yao Xiao,Yu-Ze An,Ning Peng,Yu Dai,Genqing Xie,Qi Guo,Hui Peng,Xiang-Hang Luo
View all publications

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