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AB119458

Recombinant Human RDH12 protein

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Recombinant Human RDH12 protein is a Human Fragment protein, in the 39 to 316 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

SDR7C2, RDH12, Retinol dehydrogenase 12, All-trans and 9-cis retinol dehydrogenase, Short chain dehydrogenase/reductase family 7C member 2

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SDS-PAGE - Recombinant Human RDH12 protein (AB119458)
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Supplier Data

SDS-PAGE - Recombinant Human RDH12 protein (AB119458)

SDS-PAGE analysis of ab119458.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q96NR8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMGKVVVITGANTGIGKETARELASRGARVYIACRDVLKGESAASEIRVDTKNSQVLVRKLDLSDTKSIRAFAEGFLAEEKQLHILINNAGVMMCPYSKTADGFETHLGVNHLGHFLLTYLLLERLKVSAPARVVNVSSVAHHIGKIPFHDLQSEKRYSRGFAYCHSKLANVLFTRELAKRLQGTGVTTYAVHPGVVRSELVRHSSLLCLLWRLFSPFVKTAREGAQTSLHCALAEGLEPLSGKYFSDCKRTWVSPRARNNKTAERLWNVSCELLGIRWE","proteinLength":"Fragment","predictedMolecularWeight":"33.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":316,"aminoAcidStart":39,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96NR8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RDH12 also known as retinol dehydrogenase 12 functions as an enzyme in the visual cycle. This protein has a molecular mass around 35 kDa. It is expressed strongly in the retina especially in photoreceptor cells where it participates actively in the conversion of retinoids. RDH12 works by catalyzing the oxidation-reduction of retinol into retinal key steps involved in vision processes. Its activity depends on the presence of cofactors such as NAD+ or NADP+ which it utilizes to carry out its enzymatic functions.
Biological function summary

RDH12 contributes to maintaining healthy visual function by participating in the visual cycle. It plays a significant role in the regeneration of 11-cis-retinal which is necessary for the function of rhodopsin a component of the phototransduction complex. Through this activity RDH12 ensures that photoreceptor cells sustain their ability to respond to light. Despite its primary focus on visual elements RDH12 is not isolated and might interact with other proteins in retinal cells contributing to broader signaling mechanisms within the eye.

Pathways

Researchers consider the visual cycle pathway as a core area of RDH12 activity. This cycle is critical for the conversion and recycling of visual pigments in photoreceptor cells. RDH12 works closely alongside other enzymes such as RPE65 and LRAT in this pathway to support visual function. Enzyme efficiency and stability in these interactions significantly impact the pathway's overall effectiveness affecting vision acuity and adaptation to light changes.

RDH12 mutations have strong implications in retinal diseases such as Leber congenital amaurosis (LCA) and retinitis pigmentosa (RP). These genetic conditions can lead to severe vision loss due to compromised photoreceptor cells. Within these diseases RDH12 connects with other visual cycle proteins like RPE65 whose mutations also associate with similar visual impairments. Understanding these interactions helps develop potential therapeutic strategies to address RDH12-related ailments.
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Specifications

Form

Liquid

Additional notes

ab119458 was purified using conventional chromatography techniques.

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General info

Function

Retinoids dehydrogenase/reductase with a clear preference for NADP. Displays high activity towards 9-cis, 11-cis and all-trans-retinal. Shows very weak activity towards 13-cis-retinol (PubMed : 12226107, PubMed : 15865448). Also exhibits activity, albeit with lower affinity than for retinaldehydes, towards lipid peroxidation products (C9 aldehydes) such as 4-hydroxynonenal and trans-2-nonenal (PubMed : 15865448, PubMed : 19686838). May play an important function in photoreceptor cells to detoxify 4-hydroxynonenal and potentially other toxic aldehyde products resulting from lipid peroxidation (PubMed : 19686838). Has no dehydrogenase activity towards steroids (PubMed : 12226107, PubMed : 15865448).

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

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Product protocols

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Target data

Retinoids dehydrogenase/reductase with a clear preference for NADP. Displays high activity towards 9-cis, 11-cis and all-trans-retinal. Shows very weak activity towards 13-cis-retinol (PubMed : 12226107, PubMed : 15865448). Also exhibits activity, albeit with lower affinity than for retinaldehydes, towards lipid peroxidation products (C9 aldehydes) such as 4-hydroxynonenal and trans-2-nonenal (PubMed : 15865448, PubMed : 19686838). May play an important function in photoreceptor cells to detoxify 4-hydroxynonenal and potentially other toxic aldehyde products resulting from lipid peroxidation (PubMed : 19686838). Has no dehydrogenase activity towards steroids (PubMed : 12226107, PubMed : 15865448).
See full target information RDH12
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