Recombinant Human RED1 protein is a Human Full Length protein, in the 1 to 741 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M D I E D E E N M S S S S T D V K E N R N L D N V S P K D G S T P G P G E G S Q L S N G G G G G P G R K R P L E E G S N G H S K Y R L K K R R K T P G P V L P K N A L M Q L N E I K P G L Q Y T L L S Q T G P V H A P L F V M S V E V N G Q V F E G S G P T K K K A K L H A A E K A L R S F V Q F P N A S E A H L A M G R T L S V N T D F T S D Q A D F P D T L F N G F E T P D K A E P P F Y V G S N G D D S F S S S G D L S L S A S P V P A S L A Q P P L P V L P P F P P P S G K N P V M I L N E L R P G L K Y D F L S E S G E S H A K S F V M S V V V D G Q F F E G S G R N K K L A K A R A A Q S A L A A I F N L H L D Q T P S R Q P I P S E G L Q L H L P Q V L A D A V S R L V L G K F G D L T D N F S S P H A R R K V L A G V V M T T G T D V K D A K V I S V S T G T K C I N G E Y M S D R G L A L N D C H A E I I S R R S L L R F L Y T Q L E L Y L N N K D D Q K R S I F Q K S E R G G F R L K E N V Q F H L Y I S T S P C G D A R I F S P H E P I L E G S R S Y T Q A G V Q W C N H G S L Q P R P P G L L S D P S T S T F Q G A G T T E P A D R H P N R K A R G Q L R T K I E S G E G T I P V R S N A S I Q T W D G V L Q G E R L L T M S C S D K I A R W N V V G I Q G S L L S I F V E P I Y F S S I I L G S L Y H G D H L S R A M Y Q R I S N I E D L P P L Y T L N K P L L S G I S N A E A R Q P G K A P N F S V N W T V G D S A I E V I N A T T G K D E L G R A S R L C K H A L Y C R W M R V H G K V P S H L L R S K I T K P N V Y H E S K L A A K E Y Q A A K A R L F T A F I K A G L G A W V E K P T E Q D Q F S L T P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can exert a proviral effect towards human immunodeficiency virus type 1 (HIV-1) and enhances its replication via both an editing-dependent and editing-independent mechanism. The former involves editing of adenosines in the 5'UTR while the latter occurs via suppression of EIF2AK2/PKR activation and function. Can inhibit cell proliferation and migration and can stimulate exocytosis. Isoform 1. Has a lower catalytic activity than isoform 2. Isoform 2. Has a higher catalytic activity than isoform 1.
Alternative names
ADAR2, DRADA2, RED1, ADARB1, Double-stranded RNA-specific editase 1, RNA-editing deaminase 1, RNA-editing enzyme 1, dsRNA adenosine deaminase
Recommended products
Recombinant Human RED1 protein is a Human Full Length protein, in the 1 to 741 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P78563-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M D I E D E E N M S S S S T D V K E N R N L D N V S P K D G S T P G P G E G S Q L S N G G G G G P G R K R P L E E G S N G H S K Y R L K K R R K T P G P V L P K N A L M Q L N E I K P G L Q Y T L L S Q T G P V H A P L F V M S V E V N G Q V F E G S G P T K K K A K L H A A E K A L R S F V Q F P N A S E A H L A M G R T L S V N T D F T S D Q A D F P D T L F N G F E T P D K A E P P F Y V G S N G D D S F S S S G D L S L S A S P V P A S L A Q P P L P V L P P F P P P S G K N P V M I L N E L R P G L K Y D F L S E S G E S H A K S F V M S V V V D G Q F F E G S G R N K K L A K A R A A Q S A L A A I F N L H L D Q T P S R Q P I P S E G L Q L H L P Q V L A D A V S R L V L G K F G D L T D N F S S P H A R R K V L A G V V M T T G T D V K D A K V I S V S T G T K C I N G E Y M S D R G L A L N D C H A E I I S R R S L L R F L Y T Q L E L Y L N N K D D Q K R S I F Q K S E R G G F R L K E N V Q F H L Y I S T S P C G D A R I F S P H E P I L E G S R S Y T Q A G V Q W C N H G S L Q P R P P G L L S D P S T S T F Q G A G T T E P A D R H P N R K A R G Q L R T K I E S G E G T I P V R S N A S I Q T W D G V L Q G E R L L T M S C S D K I A R W N V V G I Q G S L L S I F V E P I Y F S S I I L G S L Y H G D H L S R A M Y Q R I S N I E D L P P L Y T L N K P L L S G I S N A E A R Q P G K A P N F S V N W T V G D S A I E V I N A T T G K D E L G R A S R L C K H A L Y C R W M R V H G K V P S H L L R S K I T K P N V Y H E S K L A A K E Y Q A A K A R L F T A F I K A G L G A W V E K P T E Q D Q F S L T P
- Accession
- P78563
- Protein length
- Full Length
- Amino acids
- 1 to 741
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can exert a proviral effect towards human immunodeficiency virus type 1 (HIV-1) and enhances its replication via both an editing-dependent and editing-independent mechanism. The former involves editing of adenosines in the 5'UTR while the latter occurs via suppression of EIF2AK2/PKR activation and function. Can inhibit cell proliferation and migration and can stimulate exocytosis.
- Subcellular localisation
- Nucleus, Nucleolus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RED1 also known as ADARB1 is a protein involved in RNA editing. It weighs approximately 73 kDa. RED1 mainly expresses itself in the central nervous system but also appears in other tissues. This protein modifies RNA molecules by catalyzing the conversion of adenosine to inosine which can alter the coding potential and metabolism of the RNA it acts upon.
- Biological function summary
The protein RED1 functions within RNA editing complexes. It co-operates with other proteins like ADAR1 to carry out its editing role. In particular RED1 affects the regulation of gene expression influencing neuronal function and development in the brain. Through its editing capabilities the protein contributes to the diversity and stability of RNA transcripts.
- Pathways
RED1 plays a role in neurotransmission and neurodevelopment pathways. It interacts with other editing proteins in the ADAR family to ensure proper RNA sequence modifications critical for brain function. The editing process that RED1 participates in impacts synaptic transmission important for neuronal communication and neural network formation.
- Associated diseases and disorders
The protein RED1 is associated with neurological disorders. Changes in RED1 expression or mutations can link to conditions such as epilepsy and schizophrenia. Aberrations in RNA editing performed by RED1 might lead to the dysfunction of proteins involved in neuronal signaling. It also connects with proteins like ADAR2 influencing the pathological progress of these conditions.
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1 product image
SDS-PAGE - Recombinant Human RED1 protein (ab157847)
ab157847 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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