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AB40031

Recombinant Human REDD-1/DDIT4 protein (His tag N-Terminus)

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Recombinant Human REDD-1/DDIT4 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 232 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, ELISA, WB, Inhib.

View Alternative Names

REDD1, RTP801, DDIT4, DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801, Protein regulated in development and DNA damage response 1, REDD-1

1 Images
SDS-PAGE - Recombinant Human REDD-1/DDIT4 protein (His tag N-Terminus) (AB40031)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human REDD-1/DDIT4 protein (His tag N-Terminus) (AB40031)

SDS-PAGE : Analysis of REDD-1/DDIT4 Recombinant Protein

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

WB, SDS-PAGE, Inhib, ELISA

applications

Biologically active

No

Accession

Q9NX09

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.905% Tris acetate, 0.0292% EDTA

storage-buffer

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Complementary Products

Primary Antibodies

AB191871

Anti-REDD-1/DDIT4 antibody [EPR18716]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-REDD-1/DDIT4 antibody [EPR18716] (AB191871)

  • 5
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Proteins & Peptides

AB74521

Recombinant Human CHOP protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human CHOP protein (His tag N-Terminus) (AB74521)

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Proteins & Peptides

AB87664

Recombinant Human Securin protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Securin protein (His tag N-Terminus) (AB87664)

  • 0
  • 0
Primary Antibodies

AB32518

Anti-IKB alpha antibody [E130]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-IKB alpha antibody [E130] (AB32518)

  • 4
  • 31
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Inhib": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":232,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9NX09","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

REDD-1 also known as DDIT4 (DNA Damage Inducible Transcript 4) is a protein involved in cellular response to stress particularly hypoxic conditions. It has a molecular weight of approximately 35 kDa. This protein is expressed in various tissues including the brain liver and muscle indicating its importance in different physiological environments. REDD-1 acts as a negative regulator of the mechanistic target of rapamycin (mTOR) signaling pathway which plays an important role in cell growth and survival.
Biological function summary

The role of REDD-1 involves its participation in cellular adaptation to stress by inhibiting mTORC1 a complex within the mTOR pathway. Through this mechanism it helps cells to survive in limiting conditions by adjusting metabolic and growth signals. REDD-1 is not part of a larger protein complex but interacts closely with components of the mTOR pathway. Its regulation ensures proper cellular function under stress making it an important point for controlling cell behavior.

Pathways

REDD-1 serves as a significant regulator in the mTOR signaling and cellular stress response pathways. REDD-1 inhibits mTORC1 activity facilitating the cellular adaptation under nutrient starvation and hypoxic conditions. This inhibition affects the phosphorylation of downstream targets like S6K1 and 4EBP1 which are important for protein synthesis. Moreover REDD-1's relationship with the AMP-activated protein kinase (AMPK) adds another layer of regulation in energy balance and metabolic control.

REDD-1's dysregulation links to cancer and neurodegenerative diseases. In cancer altered REDD-1 expression affects mTORC1 signaling which can lead to unregulated cell growth and proliferation. The protein is connected to hypoxia-inducible factor (HIF) which contributes to tumor growth in hypoxic environments. In neurodegenerative diseases improper stress responses involving REDD-1 may exacerbate neuronal damage. Understanding REDD-1's interactions with mTORC1 and HIF could offer insights into potential therapeutic targets for these conditions.
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Specifications

Form

Liquid

Additional notes

Purified via His tag.

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General info

Function

Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (By similarity). Required for mTORC1-mediated defense against viral protein synthesis and virus replication (By similarity). Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.

Sequence similarities

Belongs to the DDIT4 family.

Post-translational modifications

Phosphorylated by GSK3B; this promotes proteasomal degradation.. Polyubiquitinated by a DCX (DDB1-CUL4A-RBX1) E3 ubiquitin-protein ligase complex with BTRC as substrate-recognition component, leading to its proteasomal degradation.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (By similarity). Required for mTORC1-mediated defense against viral protein synthesis and virus replication (By similarity). Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.
See full target information DDIT4
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Product promise

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