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AB135012

Recombinant human Renin protein

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Recombinant human Renin protein is a Human Full Length protein, in the 67 to 406 aa range with >80% purity and suitable for SDS-PAGE and Functional studies.The predicted molecular weight of ab135012 protein is 39 kDa.

- Save time and ensure accurate results - use our recombinant human Renin as a control
- Use to study enzyme kinetics, screening inhibitors and selectivity profiling

View Alternative Names

Renin, Angiotensinogenase, REN

2 Images
Functional Studies - Recombinant human Renin protein (AB135012)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Renin protein (AB135012)

Specific activity of ab135012 was determined to be 255 pmol/min/μg

SDS-PAGE - Recombinant human Renin protein (AB135012)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Renin protein (AB135012)

Key facts

Purity

>80% SDS-PAGE

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Activity: 255 pmol/min/μg.

Assay conditions: activity was measured with Renin titrated from 8 ng/rxn -0.125 ng/rxn.

Accession

P00797

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Preservative: 1.36% Imidazole Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Useful for the study of enzyme kinetics,screening inhibitors, and selectivityprofiling.</p>" } } }
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Product details

Ensure the validity of your result using our bioactive recombinant human Renin/Angiotensinogenase protein ab135012 as control in SDS-PAGE.


Check out our protein gel staining guide for SDS-PAGE here
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Sequence info

[{"sequence":"LTLGNTTSSVILTNYMDTQYYGEIGIGTPPQTFKVVFDTGSSNVWVPSSKCSRLYTACVYHKLFDASDSSSYKHNGTELTLRYSTGTVSGFLSQDIITVGGITVTQMFGEVTEMPALPFMLAEFDGVVGMGFIEQAIGRVTPIFDNIISQGVLKEDVFSFYYNRDSENSQSLGGQIVLGGSDPQHYEGNFHYINLIKTGVWQIQMKGVSVGSSTLLCEDGCLALVDTGASYISGSTSSIEKLMEALGAKKRLFDYVVKCNEGPTLPDISFHLGGKEYTLTSADYVFQESYSSKKLCTLAIHAMDIPPPTGPTWALGATFIRKFYTEFDRRNNRIGFALAR","proteinLength":"Full Length","predictedMolecularWeight":"39 kDa","actualMolecularWeight":null,"aminoAcidEnd":406,"aminoAcidStart":67,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P00797","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Renin also known as angiotensinogenase is a proteolytic enzyme with a molecular weight of approximately 40 kDa. It is mainly expressed in the juxtaglomerular cells of the kidneys. Renin plays an important role in the renin-angiotensin system where it initiates the conversion of angiotensinogen to angiotensin I. This process is the first step in a cascade leading to a series of effects in blood pressure regulation and fluid balance.
Biological function summary

The renin enzyme is responsible for blood pressure control and electrolyte homeostasis. It acts as a significant regulator within the renin-angiotensin aldosterone system (RAAS). By catalyzing the formation of angiotensin I from angiotensinogen renin sets off a sequence of reactions that influence vascular tone and sodium reabsorption. The activity of renin significantly interacts with various cardiovascular functions alongside complex regulatory feedback from other hormones such as aldosterone.

Pathways

Renin is a pivotal part of RAAS and interlinks with multiple cardiovascular pathways. The RAAS pathway involves angiotensinogen renin angiotensin I converting enzyme (ACE) and angiotensin II. Angiotensin II then acts on its receptor to mediate vasoconstriction and aldosterone secretion. The direct relationship between renin and ACE places this enzyme in the center of blood pressure management and has implications on kidney function and homeostasis.

High renin activity has connections with hypertension and cardiovascular diseases. Elevated renin levels contribute to increased angiotensin II production causing vasoconstriction and blood pressure elevation. Disorders like renal hypertension see direct involvement of the renin-angiotensin system. Therapeutic approaches often target RAAS with angiotensin-converting enzyme inhibitors (ACE inhibitors) and angiotensin receptor blockers aiming to mitigate the renin-related impact on these diseases.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Sequence similarities

Belongs to the peptidase A1 family.

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Product protocols

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Target data

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
See full target information REN
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Complementary Products

Primary Antibodies

AB212197

Anti-Renin antibody [EPR20693]

Advanced Validation|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (AB212197)

  • 5
  • 8
Assay Kits

AB138875

Renin Assay Kit (Fluorometric)

Functional Studies - Renin Assay Kit (Fluorometric) (AB138875)

  • 0
  • 0
Reagents, Controls & Accessories

AB171529

450 nm Stop Solution for TMB Substrate

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15

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