JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB196086

Recombinant Human Repulsive Guidance Molecule A protein (His tag)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human Repulsive Guidance Molecule A protein (His tag) is a Human Fragment protein, in the 169 to 422 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

View Alternative Names

RGM, RGMA, Repulsive guidance molecule A, RGM domain family member A

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, HPLC

applications

Biologically active

No

Accession

Q96B86

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: PBS, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MNHKVHHHHHHMPHLRTFTDRFQTCKVQGAWPLIDNNYLNVQVTNTPVLPGSAATATSKLTIIFKNFQECVDQKVYQAEMDELPAAFVDGSKNGGDKHGANSLKITEKVSGQHVEIQAKYIGTTIVVRQVGRYLTFAVRMPEEVVNAVEDWDSQGLYLCLRGCPLNQQIDFQAFHTNAEGTGARRLAAASPAPTAPETFPYETAVAKCKEKLPVEDLYYQACVFDLLTTGDVNFTLAAYYALEDVKMLHSNKDKLHLYERTRDLPG","proteinLength":"Fragment","predictedMolecularWeight":"29.82 kDa","actualMolecularWeight":null,"aminoAcidEnd":422,"aminoAcidStart":169,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96B86","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Repulsive Guidance Molecule A (RGMa) also known as RGM domain family member A is a glycoprotein involved in axon guidance and neuronal differentiation. It has a molecular weight of approximately 37 kDa. RGMa is expressed broadly in the central nervous system particularly in developing nervous tissues. Beyond the CNS RGMa is also found in various non-neuronal tissues. Its role in axonal repulsion is facilitated by its interaction with the Neogenin receptor influencing the directionality and pathfinding of growing nerve fibers.
Biological function summary

RGMa plays a role in maintaining the structural organization of the nervous system by preventing aberrant nerve growth. RGMa forms part of a larger signaling complex that influences cell differentiation and migration. It acts by binding to Neogenin and recruiting co-receptors such as bone morphogenetic proteins (BMP) which regulate the differentiation and proliferation of neural progenitor cells. This interaction highlights its involvement in maintaining proper neural architecture and functionality.

Pathways

RGMa engages in the BMP signaling pathway and the axon guidance pathway. Its interaction with BMP proteins affects the Smad signaling cascade ultimately influencing gene expression essential for neuronal differentiation. Furthermore RGMa's influence on axon guidance involves coordination with other guidance cues that help establish neural circuitry during development. Its functional relationship with Neogenin and BMP signaling pathways highlights its regulatory role in neural patterning.

RGMa is linked to conditions such as spinal cord injury and multiple sclerosis. In the context of spinal cord injury increased expression of RGMa contributes to the inhibition of neural regeneration due to its repulsive signaling effects. It interacts with other proteins including Neogenin to exacerbate the inhibitory environment post-injury. In multiple sclerosis RGMa regulates immune cell infiltration into the CNS linking it to disease progression and pathology. Understanding RGMa's role opens potential therapeutic avenues to modulate its activity in these disorders.
style
left-column

Specifications

Form

Liquid

Additional notes

The purity of ab196086 is greater than 95%, as determined by SEC-HPLC and reducing SDS-PAGE.

style
left-column

General info

Function

Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.

Sequence similarities

Belongs to the repulsive guidance molecule (RGM) family.

Post-translational modifications

Autocatalytically cleaved at low pH; the two chains remain linked via two disulfide bonds.

style
left-column

Product protocols

style
left-column

Target data

Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.
See full target information RGMA
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB307652

Anti-Repulsive guidance molecule A antibody [EPR26279-90]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Repulsive guidance molecule A antibody [EPR26279-90] (AB307652)

  • 0
  • 0
Assay Kits

AB243687

Mouse RGMA ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse RGMA ELISA Kit (AB243687)

  • 0
  • 0
Proteins & Peptides

AB177643

Recombinant Human CD1E protein (denatured)

SDS-PAGE - Recombinant Human CD1E protein (denatured) (AB177643)

  • 0
  • 0
Proteins & Peptides

AB123188

Recombinant Human RPL8 protein

SDS-PAGE - Recombinant Human RPL8 protein (AB123188)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com