Recombinant human Ret (mutated V804M) + KIF5B protein (Active) is a Human Fragment protein, in the 1 to 575 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M A D L A E C N I K V M C R F R P L N E S E V N R G D K Y I A K F Q G E D T V V I A S K P Y A F D R V F Q S S T S Q E Q V Y N D C A K K I V K D V L E G Y N G T I F A Y G Q T S S G K T H T M E G K L H D P E G M G I I P R I V Q D I F N Y I Y S M D E N L E F H I K V S Y F E I Y L D K I R D L L D V S K T N L S V H E D K N R V P Y V K G C T E R F V C S P D E V M D T I D E G K S N R H V A V T N M N E H S S R S H S I F L I N V K Q E N T Q T E Q K L S G K L Y L V D L A G S E K V S K T G A E G A V L D E A K N I N K S L S A L G N V I S A L A E G S T Y V P Y R D S K M T R I L Q D S L G G N C R T T I V I C C S P S S Y N E S E T K S T L L F G Q R A K T I K N T V C V N V E L T A E Q W K K K Y E K E K E K N K I L R N T I Q W L E N E L N R W R N G E T V P I D E Q F D K E K A N L E A F T V D K D I T L T N D K P A T A I G V I G N F T D A E R R K C E E E I A K L Y K Q L D D K D E E I N Q Q S Q L V E K L K T Q M L D Q E E L L A S T R R D Q D N M Q A E L N R L Q A E N D A S K E E V K E V L Q A L E E L A V N Y D Q K S Q E V E D K T K E Y E L L S D E L N Q K S A T L A S I D A E L Q K L K E M T N H Q K K R A A E M M A S L L K D L A E I G I A V G N N D V K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (By similarity). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes (PubMed:20386726). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity). Through binding with PLEKHM2 and ARL8B, directs lysosome movement toward microtubule plus ends (Probable). Involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (PubMed:24088571).
Additional Targets
KIF5B
Alternative names
KNS, KNS1, KIF5B, Kinesin-1 heavy chain, Conventional kinesin heavy chain, Ubiquitous kinesin heavy chain, UKHC
Recommended products
Recombinant human Ret (mutated V804M) + KIF5B protein (Active) is a Human Fragment protein, in the 1 to 575 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab268709 was determined to be 40 nmol/min/mg in a kinase assay using IGF1Rtide (KKKSPGEYVNIEFG) synthetic peptide substrate.
- Accession
- P33176
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M A D L A E C N I K V M C R F R P L N E S E V N R G D K Y I A K F Q G E D T V V I A S K P Y A F D R V F Q S S T S Q E Q V Y N D C A K K I V K D V L E G Y N G T I F A Y G Q T S S G K T H T M E G K L H D P E G M G I I P R I V Q D I F N Y I Y S M D E N L E F H I K V S Y F E I Y L D K I R D L L D V S K T N L S V H E D K N R V P Y V K G C T E R F V C S P D E V M D T I D E G K S N R H V A V T N M N E H S S R S H S I F L I N V K Q E N T Q T E Q K L S G K L Y L V D L A G S E K V S K T G A E G A V L D E A K N I N K S L S A L G N V I S A L A E G S T Y V P Y R D S K M T R I L Q D S L G G N C R T T I V I C C S P S S Y N E S E T K S T L L F G Q R A K T I K N T V C V N V E L T A E Q W K K K Y E K E K E K N K I L R N T I Q W L E N E L N R W R N G E T V P I D E Q F D K E K A N L E A F T V D K D I T L T N D K P A T A I G V I G N F T D A E R R K C E E E I A K L Y K Q L D D K D E E I N Q Q S Q L V E K L K T Q M L D Q E E L L A S T R R D Q D N M Q A E L N R L Q A E N D A S K E E V K E V L Q A L E E L A V N Y D Q K S Q E V E D K T K E Y E L L S D E L N Q K S A T L A S I D A E L Q K L K E M T N H Q K K R A A E M M A S L L K D L A E I G I A V G N N D V K
- Accession
- P33176
- Protein length
- Fragment
- Amino acids
- 1 to 575
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (By similarity). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes (PubMed:20386726). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity). Through binding with PLEKHM2 and ARL8B, directs lysosome movement toward microtubule plus ends (Probable). Involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (PubMed:24088571).
- Sequence similarities
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
- Subcellular localisation
- Cytoskeleton, Cytolytic granule membrane, Lysosome membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target known as KIF5B-RET is a fusion protein that results from the rearrangement between the KIF5B (kinesin family member 5B) gene and the RET (rearranged during transfection) proto-oncogene. This fusion protein retains the motor domain of KIF5B and the tyrosine kinase domain of RET. KIF5B has a molecular mass of approximately 116 kDa and RET is about 170 kDa when forming its normal protein product. KIF5B is largely expressed in neuronal tissues while RET is commonly found in tissues of the embryonic kidney neural crest derivatives and other neuroepithelial-derived tissues.
- Biological function summary
The formation of the KIF5B-RET fusion results in a constitutively active tyrosine kinase leading to unregulated cell signaling. KIF5B-RET is not known to be part of a larger stable protein complex but it initiates signaling pathways essential for cell proliferation and differentiation. The fusion protein hijacks the motor function of KIF5B to position RET inappropriately within the cell which leads to constitutive activation of downstream signaling cascades.
- Pathways
The constitutive activity of the KIF5B-RET fusion protein impacts the MAPK (mitogen-activated protein kinase) and PI3K-AKT (phosphoinositide 3-kinase/protein kinase B) signaling pathways. These pathways are critical for regulating cell growth and survival and the KIF5B-RET fusion protein abnormally stimulates them. The natural RET protein associates with ligands such as GDNF (glial cell-derived neurotrophic factor) but the fusion bypasses the need for these ligands resulting in active signaling independent of external cues.
- Associated diseases and disorders
KIF5B-RET is prominently associated with certain types of lung cancer specifically non-small cell lung cancer (NSCLC) and thyroid cancer particularly papillary thyroid carcinoma. The presence of this fusion leads to uncontrolled proliferation of cancerous cells. RET is also connected to other proteins such as ALK (anaplastic lymphoma kinase) in these cancers as they both drive oncogenic pathways leading to tumor development and progression. Identifying KIF5B-RET fusions in tumors has led to targeted therapies aimed at inhibiting its kinase activity.
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2 product images
Functional Studies - Recombinant human Ret (mutated V804M) + KIF5B protein (Active) (ab268709)
The specific activity of ab268709 was determined to be 40 nmol/min/mg in a kinase assay using IGF1Rtide (KKKSPGEYVNIEFG) synthetic peptide substrate.
SDS-PAGE - Recombinant human Ret (mutated V804M) + KIF5B protein (Active) (ab268709)
SDS-PAGE analysis of ab268709.
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