Recombinant human Ret + PRKAR1A protein is a Human protein, in the 1 to 236 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >85% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M E S G S T A A S E E A R S L R E C E L Y V Q K H N I Q A L L K D S I V Q L C T A R P E R P M A F L R E Y F E R L E K E E A K Q I Q N L Q K A G T R T D S R E D E I S P P P P N P V V K G R R R R G A I S A E V Y T E E D A A S Y V R K V I P K D Y K T M A A L A K A I E K N V L F S H L D D N E R S D I F D A M F S V S F I A G E T V I Q Q G D E G D N F Y V I D Q G E T D V Y V N N E W A T S V G E G G S F G E L A L I Y G T P R A A T V K A K T N V K L W G I D R D S Y R R I L M < b r > < b r > E D P K W E F P R K N L V L G K T L G E G E F G K V V K A T A F H L K G R A G Y T T V A V K M L K E N A S P S E L R D L L S E F N V L K Q V N H P H V I K L Y G A C S Q D G P L L L I V E Y A K Y G S L R G F L R E S R K V G P G Y L G S G G S R N S S S L D H P D E R A L T M G D L I S F A W Q I S Q G M Q Y L A E M K L V H R D L A A R N I L V A E G R K M K I S D F G L S R D V Y E E D S Y V K R S Q G R I P V K W M A I E S L F D H I Y T T Q S D V W S F G V L L W E I V T L G G N P Y P G I P P E R L F N L L K T G H R M E R P D N C S E E M Y R L M L Q C W K Q E P D K R P V F A D I S K D L E K M M V K R R D Y L D L A A S T P S D S L I Y D D G L S E E E T P L V D C N N A P L P R A L P S T W I E N K L Y G R I S H A F T R F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation in response to glia cell line-derived growth family factors (GDNF, NRTN, ARTN, PSPN and GDF15) (PubMed:20064382, PubMed:20616503, PubMed:20702524, PubMed:21357690, PubMed:21454698, PubMed:24560924, PubMed:28846097, PubMed:28846099, PubMed:28953886, PubMed:31118272). In contrast to most receptor tyrosine kinases, RET requires not only its cognate ligands but also coreceptors, for activation (PubMed:21994944, PubMed:23333276, PubMed:28846097, PubMed:28846099, PubMed:28953886). GDNF ligands (GDNF, NRTN, ARTN, PSPN and GDF15) first bind their corresponding GDNFR coreceptors (GFRA1, GFRA2, GFRA3, GFRA4 and GFRAL, respectively), triggering RET autophosphorylation and activation, leading to activation of downstream signaling pathways, including the MAPK- and AKT-signaling pathways (PubMed:21994944, PubMed:23333276, PubMed:24560924, PubMed:25242331, PubMed:28846097, PubMed:28846099, PubMed:28953886). Acts as a dependence receptor via the GDNF-GFRA1 signaling: in the presence of the ligand GDNF in somatotrophs within pituitary, promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF (PubMed:20616503, PubMed:21994944). Required for the molecular mechanisms orchestration during intestine organogenesis via the ARTN-GFRA3 signaling: involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue (By similarity). Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses (PubMed:28846097, PubMed:28846099, PubMed:28953886). Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner (PubMed:20702524, PubMed:21357690). Also active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage (PubMed:21357690). Triggers the differentiation of rapidly adapting (RA) mechanoreceptors (PubMed:20064382). Involved in the development of the neural crest (By similarity). Regulates nociceptor survival and size (By similarity). Phosphorylates PTK2/FAK1 (PubMed:21454698). Isoform 1. Isoform 1 in complex with GFRAL induces higher activation of MAPK-signaling pathway than isoform 2 in complex with GFRAL.
Additional Targets
PRKAR1A
Alternative names
CDHF12, CDHR16, PTC, RET51, RET, Proto-oncogene tyrosine-protein kinase receptor Ret, Cadherin family member 12, Proto-oncogene c-Ret
Recommended products
Recombinant human Ret + PRKAR1A protein is a Human protein, in the 1 to 236 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >85% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity is 280 nmol/min/mg.
- Accession
- P07949-2
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M E S G S T A A S E E A R S L R E C E L Y V Q K H N I Q A L L K D S I V Q L C T A R P E R P M A F L R E Y F E R L E K E E A K Q I Q N L Q K A G T R T D S R E D E I S P P P P N P V V K G R R R R G A I S A E V Y T E E D A A S Y V R K V I P K D Y K T M A A L A K A I E K N V L F S H L D D N E R S D I F D A M F S V S F I A G E T V I Q Q G D E G D N F Y V I D Q G E T D V Y V N N E W A T S V G E G G S F G E L A L I Y G T P R A A T V K A K T N V K L W G I D R D S Y R R I L M < b r > < b r > E D P K W E F P R K N L V L G K T L G E G E F G K V V K A T A F H L K G R A G Y T T V A V K M L K E N A S P S E L R D L L S E F N V L K Q V N H P H V I K L Y G A C S Q D G P L L L I V E Y A K Y G S L R G F L R E S R K V G P G Y L G S G G S R N S S S L D H P D E R A L T M G D L I S F A W Q I S Q G M Q Y L A E M K L V H R D L A A R N I L V A E G R K M K I S D F G L S R D V Y E E D S Y V K R S Q G R I P V K W M A I E S L F D H I Y T T Q S D V W S F G V L L W E I V T L G G N P Y P G I P P E R L F N L L K T G H R M E R P D N C S E E M Y R L M L Q C W K Q E P D K R P V F A D I S K D L E K M M V K R R D Y L D L A A S T P S D S L I Y D D G L S E E E T P L V D C N N A P L P R A L P S T W I E N K L Y G R I S H A F T R F
- Accession
- P10644
- Predicted molecular weight
- 105 kDa
- Amino acids
- 1 to 236
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation in response to glia cell line-derived growth family factors (GDNF, NRTN, ARTN, PSPN and GDF15) (PubMed:20064382, PubMed:20616503, PubMed:20702524, PubMed:21357690, PubMed:21454698, PubMed:24560924, PubMed:28846097, PubMed:28846099, PubMed:28953886, PubMed:31118272). In contrast to most receptor tyrosine kinases, RET requires not only its cognate ligands but also coreceptors, for activation (PubMed:21994944, PubMed:23333276, PubMed:28846097, PubMed:28846099, PubMed:28953886). GDNF ligands (GDNF, NRTN, ARTN, PSPN and GDF15) first bind their corresponding GDNFR coreceptors (GFRA1, GFRA2, GFRA3, GFRA4 and GFRAL, respectively), triggering RET autophosphorylation and activation, leading to activation of downstream signaling pathways, including the MAPK- and AKT-signaling pathways (PubMed:21994944, PubMed:23333276, PubMed:24560924, PubMed:25242331, PubMed:28846097, PubMed:28846099, PubMed:28953886). Acts as a dependence receptor via the GDNF-GFRA1 signaling: in the presence of the ligand GDNF in somatotrophs within pituitary, promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF (PubMed:20616503, PubMed:21994944). Required for the molecular mechanisms orchestration during intestine organogenesis via the ARTN-GFRA3 signaling: involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue (By similarity). Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses (PubMed:28846097, PubMed:28846099, PubMed:28953886). Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner (PubMed:20702524, PubMed:21357690). Also active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage (PubMed:21357690). Triggers the differentiation of rapidly adapting (RA) mechanoreceptors (PubMed:20064382). Involved in the development of the neural crest (By similarity). Regulates nociceptor survival and size (By similarity). Phosphorylates PTK2/FAK1 (PubMed:21454698).
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family.
- Post-translational modifications
Autophosphorylated on C-terminal tyrosine residues upon ligand stimulation.
- Subcellular localisation
- Endosome membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ret is a receptor tyrosine kinase with a molecular weight of approximately 150 kDa. It is also known as RET proto-oncogene and is expressed in cells of the nervous system including those in the kidney and gastrointestinal tract. Mechanically Ret functions by binding to its ligands through a complex with co-receptors called GFRα. Once activated it phosphorylates itself and downstream proteins initiating signaling cascades important for cell functions.
- Biological function summary
Ret plays important roles in cell proliferation differentiation and migration. It often forms complexes with co-receptors which dictate its specific signaling capabilities. Ret is important for normal development of tissues like the enteric nervous system and kidneys. PRKAR1A also known as protein kinase A regulatory subunit 1-alpha regulates protein kinase A (PKA) activity. This regulatory subunit helps modulate the cAMP-dependent signal transduction pathway affecting many cellular processes such as glycogen metabolism and transcription regulation.
- Pathways
Ret and PRKAR1A show significant roles in the MAPK/ERK and cAMP signaling pathways. These pathways are important for transmitting extracellular signals into intracellular responses affecting gene expression and cell cycle progression. In the MAPK/ERK pathway Ret interacts with downstream signaling proteins like SHC and GRB2 to promote ERK activation. Meanwhile PRKAR1A regulates the cAMP signaling pathway's influence on physiological processes through its control of PKA activity.
- Associated diseases and disorders
Abnormalities in Ret are associated with multiple endocrine neoplasia type 2 and Hirschsprung's disease. Mutations lead to inappropriate activation of signaling pathways such as interactions with MEN2-related oncogenic proteins driving tumorigenesis. Similarly PRKAR1A mutations are implicated in Carney complex a disorder characterized by spotty skin pigmentation myxomas and endocrine tumors. Both Ret and PRKAR1A have critical impacts on diseases primarily associated with growth and differentiation abnormalities.
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2 product images
Functional Studies - Recombinant human Ret + PRKAR1A protein (ab204148)
Sample Kinase Activity Plot. ab204148 specific activity was determined to be 280 nmol/min/mg.
SDS-PAGE - Recombinant human Ret + PRKAR1A protein (ab204148)
SDS-PAGE analysis of ab204148. MW 105 KDa.
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