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AB276552

Recombinant Human Reticulocalbin 3 protein (His tag)

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(1 Publication)

Recombinant Human Reticulocalbin 3 protein (His tag) is a Human Fragment protein, in the 1 to 324 aa range, expressed in HEK 293 cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

UNQ239/PRO272, RCN3, Reticulocalbin-3, EF-hand calcium-binding protein RLP49

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SDS-PAGE - Recombinant Human Reticulocalbin 3 protein (His tag) (AB276552)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Reticulocalbin 3 protein (His tag) (AB276552)

SDS-PAGE analysis of ab276552

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q96D15

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 100% PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MMWRPSVLLLLLLLRHGAQGKPSPDAGPHGQGRVHQAAPLSDAPHDDAHGNFQYDHEAFLGREVAKEFDQLTPEESQARLGRIVDRMDRAGDGDGWVSLAELRAWIAHTQQRHIRDSVSAAWDTYDTDRDGRVGWEELRNATYGHYAPGEEFHDVEDAETYKKMLARDERRFRVADQDGDSMATREELTAFLHPEEFPHMRDIVIAETLEDLDRNKDGYVQVEEYIADLYSAEPGEEEPAWVQTERQQFRDFRDLNKDGHLDGSEVGHWVLPPAQDQPLVEANHLLHESDTDKDGRLSKAEILGNWNMFVGSQATNYGEDLTRH","proteinLength":"Fragment","predictedMolecularWeight":"36.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":324,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q96D15","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Reticulocalbin 3 also known as ERC-55 or RCN3 is a calcium-binding protein localized in the endoplasmic reticulum. It has a molecular mass of around 44 kDa. Reticulocalbin 3 is expressed in various tissues with strong expression in the heart liver and skeletal muscles. It binds calcium through its EF-hand domains which are responsible for its ability to interact with other calcium-dependent proteins and ions. This makes it an important component of maintaining calcium homeostasis within the endoplasmic reticulum.
Biological function summary

Reticulocalbin 3 plays a role in calcium-dependent signaling processes that affect protein folding and secretion. It is part of a complex involved in glycoprotein maturation and quality control within the endoplasmic reticulum. Reticulocalbin 3 helps ensure proper protein folding which is essential for cellular function. By influencing endoplasmic reticulum stress responses Reticulocalbin 3 contributes to overall cellular health and functionality.

Pathways

The role of Reticulocalbin 3 can be seen in the endoplasmic reticulum-associated degradation (ERAD) pathway and the unfolded protein response (UPR). These pathways help manage protein misfolding and cellular stress levels. Within these pathways Reticulocalbin 3 interacts with proteins like calreticulin and calnexin which are also involved in calcium binding and protein folding mechanisms. Its function supports the regulation and maintenance of protein quality control aiding in the survival of stressed cells.

Reticulocalbin 3 shows a connection with diseases involving improper protein folding such as cystic fibrosis and some forms of liver disease. For instance in cystic fibrosis defective protein folding affects the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Reticulocalbin 3 may interact with proteins such as BiP/GRP78 involved in maintaining proper protein conformation. By stabilizing protein folding pathways Reticulocalbin 3 potentially influences the progression and severity of these conditions making it an interesting target for therapeutic intervention.
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Specifications

Form

Lyophilized

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General info

Function

Probable molecular chaperone assisting protein biosynthesis and transport in the endoplasmic reticulum (PubMed : 16433634, PubMed : 28939891). Required for the proper biosynthesis and transport of pulmonary surfactant-associated protein A/SP-A, pulmonary surfactant-associated protein D/SP-D and the lipid transporter ABCA3 (By similarity). By regulating both the proper expression and the degradation through the endoplasmic reticulum-associated protein degradation pathway of these proteins plays a crucial role in pulmonary surfactant homeostasis (By similarity). Has an anti-fibrotic activity by negatively regulating the secretion of type I and type III collagens (PubMed : 28939891). This calcium-binding protein also transiently associates with immature PCSK6 and regulates its secretion (PubMed : 16433634).

Sequence similarities

Belongs to the CREC family.

Post-translational modifications

Degraded by PCSK6 and other endoproteases including FURIN and PCSK5.. N-glycosylated.

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Product protocols

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Target data

Probable molecular chaperone assisting protein biosynthesis and transport in the endoplasmic reticulum (PubMed : 16433634, PubMed : 28939891). Required for the proper biosynthesis and transport of pulmonary surfactant-associated protein A/SP-A, pulmonary surfactant-associated protein D/SP-D and the lipid transporter ABCA3 (By similarity). By regulating both the proper expression and the degradation through the endoplasmic reticulum-associated protein degradation pathway of these proteins plays a crucial role in pulmonary surfactant homeostasis (By similarity). Has an anti-fibrotic activity by negatively regulating the secretion of type I and type III collagens (PubMed : 28939891). This calcium-binding protein also transiently associates with immature PCSK6 and regulates its secretion (PubMed : 16433634).
See full target information RCN3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Respiratory research 24:222 PubMed37710230

2023

TGFβ1-RCN3-TGFBR1 loop facilitates pulmonary fibrosis by orchestrating fibroblast activation.

Applications

Unspecified application

Species

Unspecified reactive species

Mingting Wu,Zhenyan Wang,Xiaoqian Shi,Danni Zan,Hong Chen,Shuqiao Yang,Fangping Ding,Liu Yang,Pingping Tan,Runlin Z Ma,Jing Wang,Lishuang Ma,Yingmin Ma,Jiawei Jin
View all publications
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