Recombinant Human REV1 protein (denatured) is a Human Fragment protein, in the 51 to 256 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G V A I Y V N G Y T D P S A E E L R K L M M L H G G Q Y H V Y Y S R S K T T H I I A T N L P N A K I K E L K G E K V I R P E W I V E S I K A G R L L S Y I P Y Q L Y T K Q S S V Q K G L S F N P V C R P E D P L P G P S N I A K Q L N N R V N H I V K K I E T E N E V K V N G M N S W N E E D E N N D F S F V D L E Q T S P G R K Q N G I P H P R G S T A I F N G H T P S S N G A L K T Q D C L V P M V N S V A S R L S P A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.
Alternative names
REV1L, REV1, DNA repair protein REV1, Alpha integrin-binding protein 80, Rev1-like terminal deoxycytidyl transferase, AIBP80
Recommended products
Recombinant Human REV1 protein (denatured) is a Human Fragment protein, in the 51 to 256 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9UBZ9-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G V A I Y V N G Y T D P S A E E L R K L M M L H G G Q Y H V Y Y S R S K T T H I I A T N L P N A K I K E L K G E K V I R P E W I V E S I K A G R L L S Y I P Y Q L Y T K Q S S V Q K G L S F N P V C R P E D P L P G P S N I A K Q L N N R V N H I V K K I E T E N E V K V N G M N S W N E E D E N N D F S F V D L E Q T S P G R K Q N G I P H P R G S T A I F N G H T P S S N G A L K T Q D C L V P M V N S V A S R L S P A
- Accession
- Q9UBZ9
- Protein length
- Fragment
- Predicted molecular weight
- 25.2 kDa
- Amino acids
- 51 to 256
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.
- Sequence similarities
Belongs to the DNA polymerase type-Y family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
REV1 also known as REV1 DNA-directed polymerase functions as a DNA polymerase specialized in translesion synthesis. Weighing approximately 125 kDa REV1 facilitates bypass of DNA lesions during replication ensuring continuous DNA synthesis. This protein acts primarily as a deoxycytidyl transferase inserting dCMP opposite damaged nucleotides. REV1 is expressed in various tissues but shows higher expression levels in testis and thymus. It is pivotal in maintaining genome stability under genotoxic stress.
- Biological function summary
REV1 operates within the context of DNA repair and a larger polymerase zeta complex working closely with other translesion DNA polymerases. REV1 interacts with other translesion synthesis proteins like POLH POLL and POLK to orchestrate the bypass of different types of DNA damage. These interactions enable REV1 to play a role in cellular responses to DNA damage and contribute to maintaining genome integrity.
- Pathways
REV1 integrates into the DNA damage response and translesion synthesis pathways. In these pathways REV1 coordinates with REV3L and REV7 forming part of the polymerase zeta complex to process lesions bypass that regular DNA polymerases cannot handle. Significantly it associates with the Fanconi anemia pathway affecting DNA repair mechanisms under stress. These collaborations help manage replication stress and stabilize cellular division.
- Associated diseases and disorders
REV1 is associated with cancer and immunodeficiency conditions. Abnormalities in its function correlate with faulty translesion synthesis leading to genomic instability implicated in cancer development. Furthermore in conjunction with BRCA1 and BRCA2 issues with REV1 may impact DNA repair fidelity linking it to breast cancer susceptibility. Understanding the role of REV1 in these disorders could lead to therapeutic interventions targeting its pathway interactions and activity.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human REV1 protein (denatured) (ab180340)
15% SDS-PAGE analysis of ab180340 (3 μg).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com