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AB156973

Recombinant Human REXO2 protein (His tag N-Terminus)

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Recombinant Human REXO2 protein (His tag N-Terminus) is a Human Full Length protein, in the 26 to 237 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

SFN, SMFN, CGI-114, REXO2, RNA exonuclease 2 homolog, Small fragment nuclease

1 Images
SDS-PAGE - Recombinant Human REXO2 protein (His tag N-Terminus) (AB156973)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human REXO2 protein (His tag N-Terminus) (AB156973)

15% SDS-PAGE analysis of ab156973 (3µg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9Y3B8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSVREGGAAMAAGESMAQRMVWVDLEMTGLDIEKDQIIEMACLITDSDLNILAEGPNLIIKQPDELLDSMSDWCKEHHGKSGLTKAVKESTITLQQAEYEFLSFVRQQTPPGLCPLAGNSVHEDKKFLDKYMPQFMKHLHYRIIDVSTVKELCRRWYPEEYEFAPKKAASHRALDDISESIKELQFYRNNIFKKKIDEKKRKIIENGENEKTVS","proteinLength":"Full Length","predictedMolecularWeight":"26.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":237,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y3B8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

REXO2 also referred to as RNase R or Oligoribonuclease is an enzyme with a molecular mass of around 26 kDa. It plays a significant role in the final steps of RNA decay. Mechanically REXO2 functions as an oligoribonuclease catalyzing the degradation of small ribonucleic acids into mononucleotides. This protein is primarily expressed in the mitochondria of the cell where it ensures the proper completion of RNA turnover.
Biological function summary

The enzyme facilitates the maintenance of cellular RNA quality. REXO2 is not a part of any larger complex but its exonuclease activity allows cells to eliminate short RNA fragments which could otherwise accumulate and lead to cellular dysfunction. Efficient removal of these fragments is essential for normal mitochondrial function and energy production within the cell.

Pathways

This enzyme is key to ensuring RNA metabolic balance. It plays a role in the RNA degradation pathway helping to process and clear unnecessary oligoribonucleotides. While REXO2 does not directly interact with other proteins in a complex it works alongside proteins such as PNPase and RNAse II which facilitate different stages of RNA degradation and processing within the cell.

REXO2 has implications in mitochondrial pathologies. Deficiencies or mutations in this enzyme can contribute to mitochondrial disorders which involve proteins such as POLG and Twinkle. Additionally its efficient RNA clearing function ties it to studies on neurodegenerative diseases where aberrant RNA processing can occur. Understanding these connections allows for deeper insight into how deviations in RNA management can translate into disease states.
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Specifications

Form

Liquid

Additional notes

ab156973 is purified using conventional chromatography techniques.

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General info

Function

3'-to-5'exoribonuclease that preferentially degrades DNA and RNA oligonucleotides composed of only two nucleotides (PubMed : 23741365, PubMed : 30926754, PubMed : 31588022, PubMed : 32365187). Binds and degrades longer oligonucleotides with a lower affinity (PubMed : 30926754, PubMed : 31588022, PubMed : 32365187). Plays dual roles in mitochondria, scavenging nanoRNAs (small RNA oligonucleotides of <5 nucleotides) that are produced by the degradosome and clearing short RNAs that are generated by RNA processing (PubMed : 30926754, PubMed : 31588022, PubMed : 32365187). Essential for correct initiation of mitochondrial transcription, degrading mitochondrial RNA dinucleotides to prevent RNA-primed transcription at non-canonical sites in the mitochondrial genome (PubMed : 31588022). Essential for embryonic development (By similarity).. Isoform 3. 3'-to-5'exoribonuclease that preferentially degrades DNA and RNA oligonucleotides composed of only two nucleotides.

Sequence similarities

Belongs to the oligoribonuclease family.

Subcellular localisation

Mitochondrion intermembrane space

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Product protocols

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Target data

3'-to-5'exoribonuclease that preferentially degrades DNA and RNA oligonucleotides composed of only two nucleotides (PubMed : 23741365, PubMed : 30926754, PubMed : 31588022, PubMed : 32365187). Binds and degrades longer oligonucleotides with a lower affinity (PubMed : 30926754, PubMed : 31588022, PubMed : 32365187). Plays dual roles in mitochondria, scavenging nanoRNAs (small RNA oligonucleotides of <5 nucleotides) that are produced by the degradosome and clearing short RNAs that are generated by RNA processing (PubMed : 30926754, PubMed : 31588022, PubMed : 32365187). Essential for correct initiation of mitochondrial transcription, degrading mitochondrial RNA dinucleotides to prevent RNA-primed transcription at non-canonical sites in the mitochondrial genome (PubMed : 31588022). Essential for embryonic development (By similarity).. Isoform 3. 3'-to-5'exoribonuclease that preferentially degrades DNA and RNA oligonucleotides composed of only two nucleotides.
See full target information REXO2
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