Recombinant Human RFK protein (His tag N-Terminus)
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Recombinant Human RFK protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.
View Alternative Names
Riboflavin kinase, ATP:riboflavin 5'-phosphotransferase, Flavokinase, RFK
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human RFK protein (His tag N-Terminus) (AB89009)
15% SDS-PAGE analysis of 3μg ab89009.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Riboflavin kinase impacts amino acid lipid and carbohydrate metabolism by regulating flavoprotein activity. These flavoproteins many of which require FMN as a cofactor participate in fundamental cellular processes. RFK itself does not form part of a larger multi-protein complex; however its activity and presence are strategically important in producing cofactors for such complexes enabling varied biochemical pathways.
Pathways
Riboflavin kinase contributes significantly to the maintenance of cellular redox balance and energy production. RFK functions in the riboflavin metabolism pathway where it produces FMN which acts as a precursor for flavin adenine dinucleotide (FAD) synthesis. The presence of FMN and FAD supports key enzymatic reactions implicating RFK in the broader context of the electron transport chain. It interacts functionally with proteins such as NADH dehydrogenase that also require FMN or FAD as cofactors.
Specifications
Form
Liquid
Additional notes
ab89009 is purified using conventional chromatography techniques.
General info
Function
Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.
Target data
Complementary Products
Product promise
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