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AB167884

Recombinant Human RFXANK protein

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Recombinant Human RFXANK protein is a Human Full Length protein, in the 1 to 237 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

ANKRA1, RFXB, RFXANK, DNA-binding protein RFXANK, Ankyrin repeat family A protein 1, Regulatory factor X subunit B, Regulatory factor X-associated ankyrin-containing protein, RFX-B

1 Images
SDS-PAGE - Recombinant Human RFXANK protein (AB167884)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human RFXANK protein (AB167884)

SDS-PAGE of ab167884 (3µg) under reducing condition and visualized by coomassie blue stain.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

O14593

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMELTQPAEDLIQTQQTPASELGDPEDPGEEAADGSDTVVLSLFPCTPEPVNPEPDASVSSPQGSSLKHSTTLTNRQRGNEVSALPATLDCDNLVNKPDERGFTPLIWASAFGEIETVRFLLEWGADPHILAKERESALSLASTGGYTDIVGLLLERDVDINIYDWNGGTPLLYAVRGNHVKCVEALLARGADLTTEADSGYTPMDLAVALGYRKVQQVIENHILKLFQSNLVPADPE","proteinLength":"Full Length","predictedMolecularWeight":"28 kDa","actualMolecularWeight":null,"aminoAcidEnd":237,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O14593","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RFXANK protein also known as regulatory factor X-associated ankyrin-containing protein is a component of the RFX complex. RFXANK has a molecular mass of approximately 27 kDa. This protein plays a mechanical role in the regulation of transcription by interacting with DNA sequences and recruiting various co-factors necessary for gene expression. RFXANK is largely expressed in immune cells where it modulates processes important for immune function.
Biological function summary

RFXANK acts as part of the RFX complex which is known for its role in the regulation of class II major histocompatibility complex (MHC) genes. These genes are significant for proper immune response allowing immune cells to present antigens and initiate defense mechanisms. RFXANK through its interaction with other proteins like RFX5 and RFXAP stabilizes the DNA binding ability of the RFX complex enabling the transcription of genes essential for antigen presentation.

Pathways

The regulatory influence of RFXANK integrates into important immune pathways such as the MHC class II antigen presentation pathway. RFXANK's partnership with RFX5 and RFXAP facilitates binding to the X-box motif of MHC class II promoters ensuring the transcription initiation required for effective immune surveillance. These relations evidence RFXANK's vital place in immune system operations particularly in adaptive immunity.

RFXANK mutations or dysregulation relate to bare lymphocyte syndrome type II a severe immunodeficiency disorder. This condition arises when RFXANK fails to properly regulate MHC class II expression compromising antigen presentation and adaptive immune response. Connections exist between RFXANK and other disease-related proteins like RFX5 which highlight disruptions at the molecular level impacting immune functionality.
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Specifications

Form

Liquid

Additional notes

ab167884 is purified using conventional chromatography techniques.

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General info

Function

Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator/CIITA. May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters (PubMed : 10072068, PubMed : 10725724, PubMed : 9806546). May also potentiate the activation of RAF1 (By similarity).. Isoform 2 is not involved in the positive regulation of MHC class II genes.

Post-translational modifications

Phosphorylated by RAF1.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator/CIITA. May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters (PubMed : 10072068, PubMed : 10725724, PubMed : 9806546). May also potentiate the activation of RAF1 (By similarity).. Isoform 2 is not involved in the positive regulation of MHC class II genes.
See full target information RFXANK
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