Recombinant Human RGS10 protein is a Human Full Length protein, in the 1 to 181 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M F N R A V S R L S R K R P P S D I H D S D G S S S S S H Q S L K S T A K W A A S L E N L L E D P E G V K R F R E F L K K E F S E E N V L F W L A C E D F K K M Q D K T Q M Q E K A K E I Y M T F L S S K A S S Q V N V E G Q S R L N E K I L E E P H P L M F Q K L Q D Q I F N L M K Y D S Y S R F L K S D L F L K H K R T E E E E E D L P D A Q T A A K R A S R I Y N T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Regulates G protein-coupled receptor signaling cascades, including signaling downstream of the muscarinic acetylcholine receptor CHRM2. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:10608901, PubMed:11443111, PubMed:18434541, PubMed:8774883, PubMed:9353196). Modulates the activity of potassium channels that are activated in response to CHRM2 signaling (PubMed:11443111). Activity on GNAZ is inhibited by palmitoylation of the G-protein (PubMed:9353196).
Alternative names
Regulator of G-protein signaling 10, RGS10
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Recombinant Human RGS10 protein is a Human Full Length protein, in the 1 to 181 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- O43665-3
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.058% Sodium chloride, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M F N R A V S R L S R K R P P S D I H D S D G S S S S S H Q S L K S T A K W A A S L E N L L E D P E G V K R F R E F L K K E F S E E N V L F W L A C E D F K K M Q D K T Q M Q E K A K E I Y M T F L S S K A S S Q V N V E G Q S R L N E K I L E E P H P L M F Q K L Q D Q I F N L M K Y D S Y S R F L K S D L F L K H K R T E E E E E D L P D A Q T A A K R A S R I Y N T
- Accession
- O43665
- Protein length
- Full Length
- Predicted molecular weight
- 23.7 kDa
- Amino acids
- 1 to 181
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab109947 was purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
General info
- Function
Regulates G protein-coupled receptor signaling cascades, including signaling downstream of the muscarinic acetylcholine receptor CHRM2. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:10608901, PubMed:11443111, PubMed:18434541, PubMed:8774883, PubMed:9353196). Modulates the activity of potassium channels that are activated in response to CHRM2 signaling (PubMed:11443111). Activity on GNAZ is inhibited by palmitoylation of the G-protein (PubMed:9353196).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RGS10 also known as Regulator of G-protein Signaling 10 is a 20 kDa protein that regulates G-protein signaling. This protein accelerates the GTPase activity of the G alpha subunits leading to termination of the G-protein signaling. RGS10 expresses mainly in the brain and immune cells where it modulates signal transduction processes. It influences cellular responses significantly due to its ability to deactivate active G-proteins by increasing their GTPase activity.
- Biological function summary
RGS10 plays a role in immune responses and neural function. It is not typically part of larger protein complexes but it interacts independently with other proteins in cellular signaling pathways. In the immune system RGS10 influences various processes such as cytokine production and leukocyte function. In neurons RGS10 contributes to maintaining neural signaling by regulating neurotransmitter-related G-protein coupled receptor pathways.
- Pathways
G-protein signaling pathways and the MAPK signaling pathway include RGS10 as an important regulatory protein. It modulates the G-protein coupled receptor signaling pathway an important aspect of cellular response to external stimuli. RGS10 also influences the PI3K-Akt signaling pathway by regulating upstream signaling events. These pathways involve proteins such as G alpha subunits and PI3 kinase which interact with RGS10 to control diverse physiological processes.
- Associated diseases and disorders
Individuals with altered RGS10 expression may have neurodegenerative diseases and inflammatory disorders. Alzheimer's disease is one condition where RGS10's regulatory control affects neuronal cell survival and tau phosphorylation a process implicated in disease progression. Regarding inflammation its interaction with NLRP3 inflammasome proteins connects RGS10 to inflammatory pathways which can lead to disorders like rheumatoid arthritis. Adjusting RGS10 levels might modulate disease mechanisms highlighting its potential therapeutic interest.
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1 product image
SDS-PAGE - Recombinant Human RGS10 protein (ab109947)
ab109947 at 3 ug on an SDS-PAGE gel (15%)
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