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AB93733

Recombinant Human RGS16 protein (His tag N-Terminus)

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Recombinant Human RGS16 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 202 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

RGSR, RGS16, Regulator of G-protein signaling 16, A28-RGS14P, Retinal-specific RGS, Retinally abundant regulator of G-protein signaling, RGS-r, hRGS-r

1 Images
SDS-PAGE - Recombinant Human RGS16 protein (His tag N-Terminus) (AB93733)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human RGS16 protein (His tag N-Terminus) (AB93733)

15% SDS Page analysis of 3ug ab93733

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O15492

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMCRTLAAFPTTCLERAKEFKTRLGIFLHKSELGCDTGSTGKFEWGSKHSKENRNFSEDVLGWRESFDLLLSSKNGVAAFHAFLKTEFSEENLEFWLACEEFKKIRSATKLASRAHQIFEEFICSEAPKEVNIDHETRELTRMNLQTATATCFDAAQGKTRTLMEKDSYPRFLKSPAYRDLAAQASAASATLSSCSLDEPSHT","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":202,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O15492","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RGS16 also known as Regulator of G-protein Signaling 16 acts as a GTPase-activating protein (GAP) specifically for Gα subunits of heterotrimeric G-proteins. By accelerating the hydrolysis of GTP to GDP RGS16 effectively turns off G-protein signaling. It has a molecular mass of approximately 23 kDa. RGS16 expresses in various tissues including the liver pancreas and brain suggesting a regulatory role in diverse physiological contexts.
Biological function summary

The G-protein signaling regulation by RGS16 affects cellular responses to extracellular signals. RGS16 is not part of a larger protein complex but interacts directly with Gα subunits to modulate their activity. Its regulatory actions facilitate precise control over signal duration contributing to processes such as cell proliferation differentiation and metabolism. These functions emphasize its importance in cellular communication and response.

Pathways

The signaling regulation by RGS16 influences the MAPK/ERK and PI3K/AKT pathways both of which are critical for regulating cell growth and survival. RGS16 interacts with G-proteins linked to receptors like GPCRs which are upstream modulators of these pathways. By influencing these pathways RGS16 therefore plays a role in controlling cell proliferation and differentiation. Another protein related to these pathways through RGS16 is Gαi which RGS16 specifically acts upon to terminate signaling.

Alterations in RGS16 expression or function can contribute to cancer and metabolic disorders such as diabetes. RGS16 influences these conditions by modulating signaling pathways that regulate cell growth and insulin signaling. In cancer RGS16 can affect tumor progression and metastasis through its interaction with the MAPK/ERK pathway. The protein also connects with insulin receptor signaling in metabolic disorders linking RGS16 to the regulation of glucose homeostasis and insulin sensitivity.
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Specifications

Form

Liquid

Additional notes

Purified using conventional chromatography techniques.

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General info

Function

Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed : 11602604, PubMed : 18434541). Plays an important role in the phototransduction cascade by regulating the lifetime and effective concentration of activated transducin alpha. May regulate extra and intracellular mitogenic signals (By similarity).

Post-translational modifications

Palmitoylated on Cys-2 and/or Cys-12.. Phosphorylated. Phosphorylation at Tyr-168 by EGFR enhances GTPase accelerating (GAP) activity toward GNAI1.

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Product protocols

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Target data

Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed : 11602604, PubMed : 18434541). Plays an important role in the phototransduction cascade by regulating the lifetime and effective concentration of activated transducin alpha. May regulate extra and intracellular mitogenic signals (By similarity).
See full target information RGS16
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