Recombinant Human RGS5 protein is a Human Full Length protein, in the 1 to 181 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M C K G L A A L P H S C L E R A K E I K I K L G I L L Q K P D S V G D L V I P Y N E K P E K P A K T Q K T S L D E A L Q W R D S L D K L L Q N N Y G L A S F K S F L K S E F S E E N L E F W I A C E D Y K K I K S P A K M A E K A K Q I Y E E F I Q T E A P K E V N I D H F T K D I T M K N L V E P S L S S F D M A Q K R I H A L M E K D S L P R F V R S E F Y Q E L I K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha (By similarity).
Alternative names
Regulator of G-protein signaling 5, RGS5
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Recombinant Human RGS5 protein is a Human Full Length protein, in the 1 to 181 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- O15539-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M C K G L A A L P H S C L E R A K E I K I K L G I L L Q K P D S V G D L V I P Y N E K P E K P A K T Q K T S L D E A L Q W R D S L D K L L Q N N Y G L A S F K S F L K S E F S E E N L E F W I A C E D Y K K I K S P A K M A E K A K Q I Y E E F I Q T E A P K E V N I D H F T K D I T M K N L V E P S L S S F D M A Q K R I H A L M E K D S L P R F V R S E F Y Q E L I K
- Accession
- O15539
- Protein length
- Full Length
- Predicted molecular weight
- 23.5 kDa
- Amino acids
- 1 to 181
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab113146 was purified using conventional chromatography.
General info
- Function
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha (By similarity).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Regulator of G-protein Signaling 5 (RGS5) also known as MSTP062 is a 20 kDa protein that modulates G-protein signaling. The protein acts by accelerating the GTPase activity of the alpha subunits of heterotrimeric G-proteins leading to the inactivation of G-protein-coupled receptor (GPCR) signaling. RGS5 shows high expression in vascular smooth muscle cells adipose tissue and the heart. The protein's localization indicates a role in the cardiovascular system and metabolism.
- Biological function summary
RGS5 regulates signal transduction pathways by acting as a negative regulator of GPCR signaling and influencing vascular tone and blood pressure. It forms part of the R4 subfamily of RGS proteins recognized for modulating G-protein activity. RGS5 impacts processes like vascular remodeling and angiogenesis by inhibiting G-protein-mediated signal transduction affecting the vascular cell response to proliferation and migration cues.
- Pathways
RGS5 plays a role in the GPCR signaling and the PI3K/AKT pathway. The protein interacts closely with G-protein alpha subunits such as Gαq and Gαi during the modulation of these pathways. This interaction helps regulate cardiovascular responses and cellular differentiation events coordinating with other RGS family members to maintain signaling balance.
- Associated diseases and disorders
RGS5 links with hypertension and cancer. In the context of hypertension altered expression of RGS5 correlates with vascular dysfunction affecting blood pressure regulation. In cancer RGS5 influences tumor angiogenesis and growth with potential interactions with proteins such as hypoxia-inducible factor 1-alpha (HIF-1α) affecting the oxygen supply to tumorous tissues. Understanding RGS5's involvement in these conditions can offer targets for therapeutic interventions.
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1 product image
SDS-PAGE - Recombinant Human RGS5 protein (ab113146)
15% SDS-PAGE analysis of ab113146 (3 μg)
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