Recombinant Human RHOB protein
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(2 Publications)
Recombinant Human RHOB protein is a Human Full Length protein, in the 1 to 193 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
ARH6, ARHB, RHOB, Rho-related GTP-binding protein RhoB, Rho cDNA clone 6, h6
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human RHOB protein (AB107139)
SDS-PAGE analysis of ab107139 (3 μg) under reducing conditions and visualized by coomassie blue stain.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RHOB influences cell shape and motility by modulating actin architecture. It is part of the Rho GTPase family which impacts diverse cellular processes such as cell proliferation survival and vesicle trafficking. RHOB operates within protein complexes that contribute to its regulatory functions. It has an important role in controlling endosome dynamics and influences the signaling pathways that respond to environmental cues.
Pathways
By affecting cytoskeletal remodeling RHOB plays a role in the Rho signaling pathway. It interacts with related proteins like RHOA and CDC42 facilitating the coordination of these pathways. Additionally RHOB is involved in the integrin signaling pathway which impacts cell adhesion and migration processes. These pathways are significant for understanding how cells move and communicate in response to external stimuli.
Specifications
Form
Liquid
Additional notes
ab107139 is purified using conventional chromatography techniques.
General info
Function
Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells.
Sequence similarities
Belongs to the small GTPase superfamily. Rho family.
Post-translational modifications
Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome.. (Microbial infection) Glycosylated at Tyr-34 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rho and leads to actin disassembly.. (Microbial infection) Glucosylated at Thr-37 by C.difficile toxins TcdA and TcdB in the colonic epithelium (PubMed:24905543). Monoglucosylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption (PubMed:24905543).
Subcellular localisation
Late endosome membrane
Target data
Publications (2)
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FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21627 PubMed33948992
2021
Applications
Unspecified application
Species
Unspecified reactive species
Journal of biomolecular screening 20:1294-9 PubMed26195453
2015
Applications
Unspecified application
Species
Unspecified reactive species
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