Recombinant Human RHOD protein is a Human Fragment protein, in the 18 to 207 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M M G S S H H H H H H S S G L V P R G S H M V K V V L V G D G G C G K T S L L M V F A D G A F P E S Y T P T V F E R Y M V N L Q V K G K P V H L H I W D T A G Q D D Y D R L R P L F Y P D A S V L L L C F D V T S P N S F D N I F N R W Y P E V N H F C K K V P I I V V G C K T D L R K D K S L V N K L R R N G L E P V T Y H R G Q E M A R S V G A V A Y L E C S A R L H D N V H A V F Q E A A E V A L S S R G R N F W R R I T Q G F C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Involved in endosome dynamics. May coordinate membrane transport with the function of the cytoskeleton. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Participates in the reorganization of actin cytoskeleton; the function seems to involve WHAMM and includes regulation of filopodia formation and actin filament bundling. Can modulate the effect of DAPK3 in reorganization of actin cytoskeleton and focal adhesion dissolution.
Alternative names
ARHD, RHOD, Rho-related GTP-binding protein RhoD, Rho-related protein HP1, RhoHP1
Recommended products
Recombinant Human RHOD protein is a Human Fragment protein, in the 18 to 207 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- O00212-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 50% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0584% EDTA, 0.00174% PMSF
Sequence info
Amino acid sequence
- M M G S S H H H H H H S S G L V P R G S H M V K V V L V G D G G C G K T S L L M V F A D G A F P E S Y T P T V F E R Y M V N L Q V K G K P V H L H I W D T A G Q D D Y D R L R P L F Y P D A S V L L L C F D V T S P N S F D N I F N R W Y P E V N H F C K K V P I I V V G C K T D L R K D K S L V N K L R R N G L E P V T Y H R G Q E M A R S V G A V A Y L E C S A R L H D N V H A V F Q E A A E V A L S S R G R N F W R R I T Q G F C
- Accession
- O00212
- Protein length
- Fragment
- Predicted molecular weight
- 23.8 kDa
- Amino acids
- 18 to 207
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab105608 is purified using conventional chromatography techniques.
General info
- Function
Involved in endosome dynamics. May coordinate membrane transport with the function of the cytoskeleton. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Participates in the reorganization of actin cytoskeleton; the function seems to involve WHAMM and includes regulation of filopodia formation and actin filament bundling. Can modulate the effect of DAPK3 in reorganization of actin cytoskeleton and focal adhesion dissolution.
- Sequence similarities
Belongs to the small GTPase superfamily. Rho family.
- Subcellular localisation
- Early endosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RHOD also known as Rho-related GTP-binding protein RhoD is a small signaling GTPase and a member of the Rho family of proteins. It has an approximate mass of 21 kDa. This protein plays a mechanical role in the regulation of the actin cytoskeleton and vesicle trafficking within cells. Expression of RHOD is observed in various tissues with higher levels found in lung liver and brain tissues. RHOD interacts directly with other proteins to facilitate intracellular transport and actin dynamics.
- Biological function summary
RHOD is involved in the rearrangement of the actin cytoskeleton and contributes to cellular processes like endosome transport and cell migration. It does not seem to function as part of a larger complex but has interactions with other Rho GTPase family members that influence cellular architecture and signal transduction. Through its role in modulating the cytoskeleton RHOD impacts cellular shape and mobility which are critical in diverse physiological contexts.
- Pathways
The protein RHOD interacts with the pathways regulating the cytoskeleton and cell motility. Specifically RHOD is implicated in the Ras homolog family pathways that control actin polymerization and membrane trafficking. It links to other signaling molecules such as RHOB and RAC1 that are key players in these systems. RHOD’s function is essential for facilitating vesicular transport processes and ensuring proper cellular responses to extracellular signals.
- Associated diseases and disorders
RHOD has connections to cancer progression and neurological disorders. Studies indicate that RHOD dysregulation might contribute to tumorigenesis by altering cell motility and invasion capabilities. Moreover misregulated RHOD expression or function can impact nerve cell signaling and structure linking it to certain neuropathological conditions. It interacts with related proteins such as RHOC which are also involved in tumor cell dynamics further highlighting its significance in pathological contexts.
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1 product image
SDS-PAGE - Recombinant Human RHOD protein (ab105608)
15% SDS-PAGE showing ab105608 (3 μg) at approximately 23.8 kDa.
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