Recombinant Human RhoV protein (denatured) is a Human Full Length protein, in the 1 to 236 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M P P R E L S E A E P P P L R A P T P P P R R R S A P P E L G I K C V L V G D G A V G K S S L I V S Y T C N G Y P A R Y R P T A L D T F S V Q V L V D G A P V R I E L W D T A G Q E D F D R L R S L C Y P D T D V F L A C F S V V Q P S S F Q N I T E K W L P E I R T H N P Q A P V L L V G T Q A D L R D D V N V L I Q L D Q G G R E G P V P Q P Q A Q G L A E K I R A C C Y L E C S A L T Q K N L K E V F D S A I L S A I E H K A R L E K K L N A K G V R T L S R C R W K K F F C F V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays a role in the control of the actin cytoskeleton via activation of the JNK pathway.
Alternative names
ARHV, WRCH2, RHOV, Rho-related GTP-binding protein RhoV, CDC42-like GTPase 2, GTP-binding protein-like 2, Rho GTPase-like protein ARHV, Wnt-1 responsive Cdc42 homolog 2, WRCH-2
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Recombinant Human RhoV protein (denatured) is a Human Full Length protein, in the 1 to 236 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q96L33-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 12.01% Urea, 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M P P R E L S E A E P P P L R A P T P P P R R R S A P P E L G I K C V L V G D G A V G K S S L I V S Y T C N G Y P A R Y R P T A L D T F S V Q V L V D G A P V R I E L W D T A G Q E D F D R L R S L C Y P D T D V F L A C F S V V Q P S S F Q N I T E K W L P E I R T H N P Q A P V L L V G T Q A D L R D D V N V L I Q L D Q G G R E G P V P Q P Q A Q G L A E K I R A C C Y L E C S A L T Q K N L K E V F D S A I L S A I E H K A R L E K K L N A K G V R T L S R C R W K K F F C F V
- Accession
- Q96L33
- Protein length
- Full Length
- Predicted molecular weight
- 28.6 kDa
- Amino acids
- 1 to 236
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Plays a role in the control of the actin cytoskeleton via activation of the JNK pathway.
- Sequence similarities
Belongs to the small GTPase superfamily. Rho family.
- Subcellular localisation
- Endosome membrane
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RhoV also known as Chp (Cdc42 homologous protein) is a member of the Rho GTPase family with a molecular mass of approximately 21 kDa. It functions as a molecular switch cycling between an active GTP-bound form and an inactive GDP-bound form. RhoV is mainly expressed in the skin hair follicles and certain cancer cell lines. The protein is involved in actin cytoskeleton organization affecting cell shape and motility.
- Biological function summary
This protein plays a role in cellular processes like morphogenesis and differentiation. RhoV interacts with other proteins in cellular signaling pathways including effector proteins like PAKs (p21-activated kinases). It is not typically associated with large protein complexes but modulates actin and microtubule arrays important for proper cell division and migration.
- Pathways
Many researchers link RhoV to the JNK (c-Jun N-terminal kinase) and ERK (extracellular signal-regulated kinase) signaling pathways. In the JNK pathway RhoV activates JNK kinases which are involved in processes like stress response and apoptosis. Additionally its interactions within the ERK pathway help regulate cell proliferation and differentiation. RhoV coordinates with proteins such as Rac1 and Cdc42 other Rho GTPases with similar functions.
- Associated diseases and disorders
RhoV shows a connection with cancer and skin-related conditions. Increased expression of RhoV may support tumor growth and metastasis in malignant melanoma. In these contexts RhoV often influences pathways associated with other proteins like Ras a well-known regulator of cell proliferation and survival in cancer cells. Researchers also explore its involvement in skin disorders related with abnormal keratinocyte differentiation. Understanding RhoV's interactions with these proteins could provide insights into its role in these conditions.
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1 product image
SDS-PAGE - Recombinant Human RhoV protein (denatured) (ab131677)
15% SDS-PAGE analysis of 3 µg ab131677.
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