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AB137138

Recombinant Human Ribonuclease 3/ECP protein (denatured) (His-DDDDK tag N-Terminus)

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Recombinant Human Ribonuclease 3/ECP protein (denatured) (His-DDDDK tag N-Terminus) is a Human Full Length protein, in the 28 to 160 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

ECP, RNS3, RNASE3, Eosinophil cationic protein, Ribonuclease 3, RNase 3

1 Images
SDS-PAGE - Recombinant Human Ribonuclease 3/ECP protein (denatured) (His-DDDDK tag N-Terminus) (AB137138)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human Ribonuclease 3/ECP protein (denatured) (His-DDDDK tag N-Terminus) (AB137138)

15% SDS-PAGE analysis of 3 µg ab137138.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His-DDDDK tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P12724

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as Ribonuclease 3
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Sequence info

[{"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSHMRPPQFTRAQWFAIQHISLNPPRCTIAMRAINNYRWRCKNQNTFLRTTFANVVNVCGNQSIRCPHNRTLNNCHRSRFRVPLLHCDLINPGAQNISNCRYADRPGRRFYVVACDNRDPRDSPRYPVVPVHLDTTI","proteinLength":"Full Length","predictedMolecularWeight":"19.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":160,"aminoAcidStart":28,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P12724","tags":[{"tag":"His-DDDDK","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ribonuclease 3 commonly known as Eosinophil Cationic Protein (ECP) is an enzyme with ribonuclease activity. Researchers sometimes refer to it as human RNase 3. The protein has a molecular mass of about 18 to 22 kDa and is highly expressed in eosinophils a type of white blood cell primarily involved in allergic responses and parasitic infections. ECP exhibits cytotoxic bactericidal and antiviral activities contributing to its diverse biological functions.
Biological function summary

Ribonuclease 3 plays a significant role in immune defense mechanisms leveraging its arginine-rich domain to interact with negatively charged microbial surfaces. This interaction allows ECP to participate in the destruction of pathogen cell walls. It is not part of a larger complex but interacts with cellular membranes making it an effective tool against a variety of pathogens. Whether acting through enzymatic activity or by disrupting membrane integrity ECP maintains a pivotal role in host defense.

Pathways

ECP influences processes in both innate and adaptive immune responses. It actively participates in the Th2 cell-mediated immune response which is critical for countering parasitic infections. This protein connects with other components like major basic protein and eosinophil peroxidase through these pathways forming an integrated network that boosts immune responses. Its presence in pathways linked to inflammation and immunity highlights its multifunctionality and importance.

ECP levels correlate with conditions such as asthma and eosinophilic esophagitis. In asthma elevated ECP levels indicate eosinophilic inflammation and airway hyperresponsiveness. Researchers have identified a connection between ECP and proteins like interleukin-5 which modulate eosinophil activity. Similarly in eosinophilic esophagitis increased ECP levels indicate ongoing inflammation and tissue damage providing insights into disease progression and severity.
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Specifications

Form

Liquid

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General info

Function

Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.

Sequence similarities

Belongs to the pancreatic ribonuclease family.

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Product protocols

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Target data

Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
See full target information RNASE3
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