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AB156743

Recombinant Human Ribonuclease H2, subunit A/RNASEH2A protein (denatured) (His tag N-Terminus)

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Recombinant Human Ribonuclease H2, subunit A/RNASEH2A protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 299 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

RNASEHI, RNHIA, RNASEH2A, Ribonuclease H2 subunit A, RNase H2 subunit A, Aicardi-Goutieres syndrome 4 protein, RNase H(35), Ribonuclease HI large subunit, Ribonuclease HI subunit A, AGS4, RNase HI large subunit

1 Images
SDS-PAGE - Recombinant Human Ribonuclease H2, subunit A/RNASEH2A protein (denatured) (His tag N-Terminus) (AB156743)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Ribonuclease H2, subunit A/RNASEH2A protein (denatured) (His tag N-Terminus) (AB156743)

15% SDS-PAGE analysis of ab156743 (3μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O75792

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as Ribonuclease H2
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMDLSELERDNTGRCRLSSPVPAVCRKEPCVLGVDEAGRGPVLGPMVYAICYCPLPRLADLEALKVADSKTLLESERERLFAKMEDTDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTATGLIQYALDQGVNVTQVFVDTVGMPETYQARLQQSFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKKWQFVEKLQDLDTDYGSGYPNDPKTKAWLKEHVEPVFGFPQFVRFSWRTAQTILEKEAEDVIWEDSASENQEGLRKITSYFLNEGSQARPRSSHRYFLERGLESATSL","proteinLength":"Full Length","predictedMolecularWeight":"35.84 kDa","actualMolecularWeight":null,"aminoAcidEnd":299,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O75792","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ribonuclease H2 subunit A also known as RNASEH2A is one part of the RNase H2 enzyme complex. This protein weighs approximately 33 kilodaltons and plays an important role in RNA-DNA hybrid processing. RNASEH2A operates by cleaving the RNA strand of RNA-DNA hybrids which is necessary for maintaining genome stability. The protein expresses in a variety of tissues. Importantly it is found in high levels in cells with rapid division like embryonic and stem cells.
Biological function summary

RNASEH2A facilitates the removal of ribonucleotides from DNA which helps in DNA replication and repair. As a part of the RNase H2 complex alongside subunits B and C it ensures the correct removal of embedded ribonucleotides from DNA strands. This process is important for maintaining the integrity of the genome and preventing mutations. The activity of RNASEH2A directly impacts DNA replication fidelity and overall genome stability.

Pathways

RNASEH2A plays an important role in the maintenance of genome integrity and the DNA repair pathway. It directly interacts with the process of ribonucleotide excision repair which prevents genomic instability. RNASEH2A works alongside enzymes involved in DNA replication and repair mechanisms like DNA polymerases to correct DNA strands. The collaboration of these proteins ensures the accuracy of genomic information passed during cell division.

RNASEH2A is associated with Aicardi-Goutières syndrome a severe neurodevelopmental disorder. This syndrome results from mutations in any of the RNase H2 subunits including RNASEH2A leading to accumulated DNA damage and a chronic interferon response. Another condition linked to malfunctions in this protein is systemic lupus erythematosus where an abnormal immune response attacks the body’s own cells. Both conditions connect RNASEH2A with the regulation of immune responses and highlight its role in preventing inappropriate autoimmunity.
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Specifications

Form

Liquid

Additional notes

ab156743 is purified by anion-exchange and gel-filtration chromatography techniques.

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General info

Function

Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA : DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA : RNA duplexes.

Sequence similarities

Belongs to the RNase HII family. Eukaryotic subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA : DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA : RNA duplexes.
See full target information RNASEH2A
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