Recombinant Human Ribonuclease Inhibitor/RAI protein is a Human Full Length protein, in the 1 to 461 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M S L D I Q S L D I Q C E E L S D A R W A E L L P L L Q Q C Q V V R L D D C G L T E A R C K D I S S A L R V N P A L A E L N L R S N E L G D V G V H C V L Q G L Q T P S C K I Q K L S L Q N C C L T G A G C G V L S S T L R T L P T L Q E L H L S D N L L G D A G L Q L L C E G L L D P Q C R L E K L Q L E Y C S L S A A S C E P L A S V L R A K P D F K E L T V S N N D I N E A G V R V L C Q G L K D S P C Q L E A L K L E S C G V T S D N C R D L C G I V A S K A S L R E L A L G S N K L G D V G M A E L C P G L L H P S S R L R T L W I W E C G I T A K G C G D L C R V L R A K E S L K E L S L A G N E L G D E G A R L L C E T L L E P G C Q L E S L W V K S C S F T A A C C S H F S S V L A Q N R F L L E L Q I S N N R L E D A G V R E L C Q G L G Q P G S V L R V L W L A D C D V S D S S C S S L A A T L L A N H S L R E L D L S N N C L G D A G I L Q L V E S V R Q P G C L L E Q L V L Y D I Y W S E E M E D R L Q A L E K D K P S L R V I S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and angiogenin (ANG) (PubMed:12578357, PubMed:14515218, PubMed:3219362, PubMed:3243277, PubMed:3470787, PubMed:9050852). May play a role in redox homeostasis (PubMed:17292889). Required to inhibit the cytotoxic tRNA ribonuclease activity of ANG in the cytoplasm in absence of stress (PubMed:23843625, PubMed:32510170). Relocates to the nucleus in response to stress, relieving inhibition of ANG in the cytoplasm, and inhibiting the angiogenic activity of ANG in the nucleus (PubMed:23843625).
Alternative names
PRI, RNH, RNH1, Ribonuclease inhibitor, Placental ribonuclease inhibitor, Ribonuclease/angiogenin inhibitor 1, Placental RNase inhibitor, RAI
Recommended products
Recombinant Human Ribonuclease Inhibitor/RAI protein is a Human Full Length protein, in the 1 to 461 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P13489-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris-HCl buffer, 0.3% Glutathione
Sequence info
Amino acid sequence
- M S L D I Q S L D I Q C E E L S D A R W A E L L P L L Q Q C Q V V R L D D C G L T E A R C K D I S S A L R V N P A L A E L N L R S N E L G D V G V H C V L Q G L Q T P S C K I Q K L S L Q N C C L T G A G C G V L S S T L R T L P T L Q E L H L S D N L L G D A G L Q L L C E G L L D P Q C R L E K L Q L E Y C S L S A A S C E P L A S V L R A K P D F K E L T V S N N D I N E A G V R V L C Q G L K D S P C Q L E A L K L E S C G V T S D N C R D L C G I V A S K A S L R E L A L G S N K L G D V G M A E L C P G L L H P S S R L R T L W I W E C G I T A K G C G D L C R V L R A K E S L K E L S L A G N E L G D E G A R L L C E T L L E P G C Q L E S L W V K S C S F T A A C C S H F S S V L A Q N R F L L E L Q I S N N R L E D A G V R E L C Q G L G Q P G S V L R V L W L A D C D V S D S S C S S L A A T L L A N H S L R E L D L S N N C L G D A G I L Q L V E S V R Q P G C L L E Q L V L Y D I Y W S E E M E D R L Q A L E K D K P S L R V I S
- Accession
- P13489
- Protein length
- Full Length
- Predicted molecular weight
- 76.45 kDa
- Amino acids
- 1 to 461
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and angiogenin (ANG) (PubMed:12578357, PubMed:14515218, PubMed:3219362, PubMed:3243277, PubMed:3470787, PubMed:9050852). May play a role in redox homeostasis (PubMed:17292889). Required to inhibit the cytotoxic tRNA ribonuclease activity of ANG in the cytoplasm in absence of stress (PubMed:23843625, PubMed:32510170). Relocates to the nucleus in response to stress, relieving inhibition of ANG in the cytoplasm, and inhibiting the angiogenic activity of ANG in the nucleus (PubMed:23843625).
- Post-translational modifications
The N-terminus is blocked.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Ribonuclease Inhibitor.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ribonuclease Inhibitor (RI) also known as RAI is a protein weighing approximately 50 kDa and is responsible for binding to ribonucleases (RNases) to inhibit their activity. This protein is expressed in a variety of tissues but appears most abundantly in the cytoplasm of mammalian cells. RI is characterized by its leucine-rich repeat (LRR) structure which facilitates its high-affinity binding to target RNases effectively serving to regulate ribonuclease activity within cells.
- Biological function summary
Ribonuclease Inhibitor participates in protecting cellular RNA from degradation by forming stable complexes with RNases. This interaction is important for maintaining RNA stability particularly within cellular environments where mRNA turnover must be tightly controlled. RI does not only inhibit free RNases but also regulates those RNases that are secreted or released subsequently. It does not have enzymatic activity itself serving more as a guard that ensures RNA integrity for processes such as transcription translation and overall gene expression.
- Pathways
The function of Ribonuclease Inhibitor fits strategically into RNA metabolism and the regulation of its turnover under various physiological conditions. RI acts within the mRNA biogenesis and degradation pathways coordinating with other proteins involved in RNA processing such as Dicer. RI's role is essential in fine-tuning RNA levels and preventing unwanted cellulalr RNA cleavage which can be directed by pathways involving diverse sets of RNases across different cellular contexts.
- Associated diseases and disorders
Researchers have observed connections between Ribonuclease Inhibitor and conditions such as cancer and neurodegenerative diseases. For instance disrupting RI balance can result in excessive RNA degradation contributing to pathologies associated with abnormal mRNA destruction in cells. It often aligns in regulatory functions with proteins like angiogenin where disrupted RI-angiogenin interactions have been implicated in tumor growth and progression. Additionally RI's dysfunction is associated with neuroinflammatory conditions where RNA stability plays critical roles.
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1 product image
SDS-PAGE - Recombinant Human Ribonuclease Inhibitor/RAI protein (ab114503)
ab114503 analysed by 12.5% SDS-PAGE and stained with Coomassie Blue.
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