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AB101632

Recombinant Human RIDA protein

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Recombinant Human RIDA protein is a Human Full Length protein, in the 1 to 137 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

HRSP12, RIDA, 2-iminobutanoate/2-iminopropanoate deaminase, 14.5 kDa translational inhibitor protein, Heat-responsive protein 12, Reactive intermediate imine deaminase A homolog, Translation inhibitor L-PSP ribonuclease, UK114 antigen homolog, hp14.5, p14.5

1 Images
SDS-PAGE - Recombinant Human RIDA protein (AB101632)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RIDA protein (AB101632)

15% SDS PAGE analysis of 3 μg ab101632.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P52758

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as HRSP12
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMSSLIRRVISTAKAPGAIGPYSQAVLVDRTIYISGQIGMDPSSGQLVSGGVAEEAKQALKNMGEILKAAGCDFTNVVKTTVLLADINDFNTVNEIYKQYFKSNFPARAAYQVAALPKGSRIEIEAVAIQGPLTTASL","proteinLength":"Full Length","predictedMolecularWeight":"16.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":137,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P52758","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIDA also known as Reactive Intermediate Deaminase A functions as an important enzyme in cellular processes. This protein has a molecular weight of approximately 19 kDa and is prominently expressed in various tissues including the liver and kidneys. RIDA acts by deaminating reactive intermediates specifically 2-aminoacrylate which in turn prevents toxic accumulation within cells. Its expression sees influence under various metabolic stress conditions indicating its important role in maintaining cellular homeostasis.
Biological function summary

Reactive intermediate deaminase A supports metabolic resilience by engaging in the nitrogen metabolism pathway. It functions as part of a deaminase complex important for processing amino acid intermediates. During this metabolic function RIDA catalyzes the conversion of potentially toxic compounds into less harmful ones facilitating their subsequent expulsion or further processing. Such activity helps maintain the balance of available nitrogenous compounds in the cell which is necessary for proper cellular function.

Pathways

Reactive intermediate deaminase A integrates into the amino acid metabolism and the central nitrogen metabolism pathways. These pathways involve several interactive proteins like serine hydroxymethyltransferase supporting the correct regulation and maintenance of cellular nitrogen balance. RIDA collaborates with these proteins to ensure that amino acid degradation is smooth and without the buildup of intermediate byproducts.

Some studies associate reactive intermediate deaminase A with metabolic disorders involving amino acid imbalances. Diseases such as hyperoxaluria and nonketotic hyperglycinemia show altered activities of proteins connected to RIDA indicating its potential role in these conditions. The connection between RIDA and other proteins like glycine decarboxylase suggests a broader impact on the intricate balance of amino acids in the human body.
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Specifications

Form

Liquid

Additional notes

ab101632 was purified using conventional chromatography.

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General info

Function

Catalyzes the hydrolytic deamination of enamine/imine intermediates that form during the course of normal metabolism. May facilitate the release of ammonia from these potentially toxic reactive metabolites, reducing their impact on cellular components. It may act on enamine/imine intermediates formed by several types of pyridoxal-5'-phosphate-dependent dehydratases including L-threonine dehydratase.. Also promotes endoribonucleolytic cleavage of some transcripts by promoting recruitment of the ribonuclease P/MRP complex (PubMed : 30930054, PubMed : 8973653). Acts by bridging YTHDF2 and the ribonuclease P/MRP complex (PubMed : 30930054). RIDA/HRSP12 binds to N6-methyladenosine (m6A)-containing mRNAs containing a 5'-GGUUC-3' motif : cooperative binding of RIDA/HRSP12 and YTHDF2 to such transcripts lead to recruitment of the ribonuclease P/MRP complex and subsequent endoribonucleolytic cleavage (PubMed : 30930054).

Sequence similarities

Belongs to the RutC family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Catalyzes the hydrolytic deamination of enamine/imine intermediates that form during the course of normal metabolism. May facilitate the release of ammonia from these potentially toxic reactive metabolites, reducing their impact on cellular components. It may act on enamine/imine intermediates formed by several types of pyridoxal-5'-phosphate-dependent dehydratases including L-threonine dehydratase.. Also promotes endoribonucleolytic cleavage of some transcripts by promoting recruitment of the ribonuclease P/MRP complex (PubMed : 30930054, PubMed : 8973653). Acts by bridging YTHDF2 and the ribonuclease P/MRP complex (PubMed : 30930054). RIDA/HRSP12 binds to N6-methyladenosine (m6A)-containing mRNAs containing a 5'-GGUUC-3' motif : cooperative binding of RIDA/HRSP12 and YTHDF2 to such transcripts lead to recruitment of the ribonuclease P/MRP complex and subsequent endoribonucleolytic cleavage (PubMed : 30930054).
See full target information RIDA
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