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AB163216

Recombinant Human RIO2 protein (GST tag N-Terminus)

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Recombinant Human RIO2 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 552 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

RIO2, RIOK2, Serine/threonine-protein kinase RIO2, RIO kinase 2

1 Images
SDS-PAGE - Recombinant Human RIO2 protein (Tagged) (AB163216)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human RIO2 protein (Tagged) (AB163216)

ab163216 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q9BVS4

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as RIOK2.
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Sequence info

[{"sequence":"MGKVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPGSLIASIASLKHGGCNKVLRELVKHKLIAWERTKTVQGYRLTNAGYDYLALKTLSSRQVVESVGNQMGVGKESDIYIVANEEGQQFALKLHRLGRTSFRNLKNKRDYHKYRHNVSWLYLSRLSAMKEFAYMKALYERKFPVPKPIDYNRHAVVMELINGYPLCQIHHVEDPASVYDEAMELIVKLANHGLIHGDFNEFNLILDESDHITMIDFPQMVSTSHPNAEWYFDRDVKCIKDFFMKRFSYESELFPTFKDIRREDTLDVEVSASGYTKEMQADDELLHPLGPDDKNIETKEGSEFSFSDGEVAEKAEVYGSENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFEMTEFNQALEEIKGQVVENNSVTEFSEEKNRTENYNRQDGQRVQGGVPAGSDEYEDECPHLIALSSLNREFRPFRDEENVGAMNQYRTRTLSITSSGSAVSCSTIPPELAKQKVKRQLTKQQKSAVRRRLQKGEANIFTKQRRENMQNIKSSLEAASFWGE","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":552,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9BVS4","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIO2 also known as RIO kinase 2 belongs to the serine/threonine-protein kinase family. This protein has a molecular weight of approximately 67 kDa. RIO2 is widely expressed across various tissues being particularly prevalent in rapidly dividing cells. It functions as an ATP-dependent kinase essential for proper biological processes that involve cellular growth and proliferation. RIO2 works in the pre-rRNA processing and ribosome biogenesis which are important for maintaining cellular function.
Biological function summary

The protein facilitates ribosome assembly showing importance in the maturation of the 40S ribosomal subunit. RIO2 operates as part of a ribosomal RNA processing complex interacting with several other proteins involved in ribosome biogenesis. By ensuring the correct processing and assembly of rRNA RIO2 supports efficient protein synthesis which is fundamental for cell survival and growth. Its role in ribosome assembly highlights its importance in sustaining cellular functions.

Pathways

RIO2 participates actively in ribosome biogenesis and interacts with various ribosomal proteins and processing factors. It associates with well-known pathways such as the biogenesis of the small ribosomal subunit and the RNA transport pathway. In these processes RIO2 functions alongside proteins like NOP14 and ENP1 assisting in the sequential steps of the ribosome assembly line. These interactions ensure that cells maintain their protein synthesis capability under different physiological states.

RIO2 has connections to cancer and ribosomopathy. Aberrant activity or expression of RIO2 is observed in several cancers where its role in ribosome assembly is disrupted leading to uncontrolled cell growth. RIO2 also interacts with proteins involved in cancer pathways such as MYC which can further drive tumorigenesis. In ribosomopathies mutations or dysregulation of RIO2 affect ribosomal function and lead to diseases with growth and developmental defects. Addressing RIO2 abnormalities might offer therapeutic strategies for these conditions.
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Specifications

Form

Liquid

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General info

Function

Serine/threonine-protein kinase involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in export of the 40S pre-ribosome particles (pre-40S) from the nucleus to the cytoplasm. Its kinase activity is required for the release of NOB1, PNO1 and LTV1 from the late pre-40S and the processing of 18S-E pre-rRNA to the mature 18S rRNA (PubMed : 19564402). Regulates the timing of the metaphase-anaphase transition during mitotic progression, and its phosphorylation, most likely by PLK1, regulates this function (PubMed : 21880710).

Sequence similarities

Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.

Post-translational modifications

Autophosphorylated (in vitro) (PubMed:19564402, PubMed:21880710). Phosphorylation at Ser-335, Ser-380, Ser-548 by PLK1 affects the timing of the metaphase-anaphase transition (PubMed:21880710).

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Product protocols

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Target data

Serine/threonine-protein kinase involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in export of the 40S pre-ribosome particles (pre-40S) from the nucleus to the cytoplasm. Its kinase activity is required for the release of NOB1, PNO1 and LTV1 from the late pre-40S and the processing of 18S-E pre-rRNA to the mature 18S rRNA (PubMed : 19564402). Regulates the timing of the metaphase-anaphase transition during mitotic progression, and its phosphorylation, most likely by PLK1, regulates this function (PubMed : 21880710).
See full target information RIOK2
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