Recombinant Human RIOK1/RIO1 protein (Tagged) is a Human Full Length protein, in the 1 to 568 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M D Y R R L L M S R V V P G Q F D D A D S S D S E N R D L K T V K E K D D I L F E D L Q D N V N E N G E G E I E D E E E E G Y D D D D D D W D W D E G V G K L A K G Y V W N G G S N P Q A N R Q T S D S S S A K M S T P A D K V L R K F E N K I N L D K L N V T D S V I N K V T E K S R Q K E A D M Y R I K D K A D R A T V E Q V L D P R T R M I L F K M L T R G I I T E I N G C I S T G K E A N V Y H A S T A N G E S R A I K I Y K T S I L V F K D R D K Y V S G E F R F R H G Y C K G N P R K M V K T W A E K E M R N L I R L N T A E I P C P E P I M L R S H V L V M S F I G K D D M P A P L L K N V Q L S E S K A R E L Y L Q V I Q Y M R R M Y Q D A R L V H A D L S E F N M L Y H G G G V Y I I D V S Q S V E H D H P H A L E F L R K D C A N V N D F F M R H S V A V M T V R E L F E F V T D P S I T H E N M D A Y L S K A M E I A S Q R T K E E R S S Q D H V D E E V F K R A Y I P R T L N E V K N Y E R D M D I I M K L K E E D M A M N A Q Q D N I L Y Q T V T G L K K D L S G V Q K V P A L L E N Q V E E R T C S D S E D I G S S E C S D T D S E E Q G D H A R P K K H T T D P D I D K K E R K K M V K E A Q R E K R K N K I P K H V K K R K E K T A K T K K G K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor (PubMed:22072790). The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit (By similarity). Despite the protein kinase domain is proposed to act predominantly as an ATPase (By similarity). The catalytic activity regulates its dynamic association with the 40S subunit (By similarity). In addition to its role in ribosomal biogenesis acts as an adapter protein by recruiting NCL/nucleolin the to PRMT5 complex for its symmetrical methylation (PubMed:21081503).
Alternative names
RIO1, RIOK1, Serine/threonine-protein kinase RIO1, RIO kinase 1
Recommended products
Recombinant Human RIOK1/RIO1 protein (Tagged) is a Human Full Length protein, in the 1 to 568 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9BRS2-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M D Y R R L L M S R V V P G Q F D D A D S S D S E N R D L K T V K E K D D I L F E D L Q D N V N E N G E G E I E D E E E E G Y D D D D D D W D W D E G V G K L A K G Y V W N G G S N P Q A N R Q T S D S S S A K M S T P A D K V L R K F E N K I N L D K L N V T D S V I N K V T E K S R Q K E A D M Y R I K D K A D R A T V E Q V L D P R T R M I L F K M L T R G I I T E I N G C I S T G K E A N V Y H A S T A N G E S R A I K I Y K T S I L V F K D R D K Y V S G E F R F R H G Y C K G N P R K M V K T W A E K E M R N L I R L N T A E I P C P E P I M L R S H V L V M S F I G K D D M P A P L L K N V Q L S E S K A R E L Y L Q V I Q Y M R R M Y Q D A R L V H A D L S E F N M L Y H G G G V Y I I D V S Q S V E H D H P H A L E F L R K D C A N V N D F F M R H S V A V M T V R E L F E F V T D P S I T H E N M D A Y L S K A M E I A S Q R T K E E R S S Q D H V D E E V F K R A Y I P R T L N E V K N Y E R D M D I I M K L K E E D M A M N A Q Q D N I L Y Q T V T G L K K D L S G V Q K V P A L L E N Q V E E R T C S D S E D I G S S E C S D T D S E E Q G D H A R P K K H T T D P D I D K K E R K K M V K E A Q R E K R K N K I P K H V K K R K E K T A K T K K G K
- Accession
- Q9BRS2
- Protein length
- Full Length
- Amino acids
- 1 to 568
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor (PubMed:22072790). The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit (By similarity). Despite the protein kinase domain is proposed to act predominantly as an ATPase (By similarity). The catalytic activity regulates its dynamic association with the 40S subunit (By similarity). In addition to its role in ribosomal biogenesis acts as an adapter protein by recruiting NCL/nucleolin the to PRMT5 complex for its symmetrical methylation (PubMed:21081503).
- Sequence similarities
Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RIOK1 also known as RIO1 is an atypical serine/threonine kinase with a molecular mass of approximately 67 kDa. It plays a role in processing small ribosomal subunit biogenesis. The protein is expressed in various tissues including liver and heart. Unlike typical kinases RIOK1 does not rely on the classical ATP-dependent mechanism for its kinase activity. It participates in the phosphorylation of some proteins which is necessary for ribosome maturation.
- Biological function summary
The kinase RIOK1/RIO1 is part of the ribosome biogenesis pathway which is essential for protein synthesis. It forms part of a larger dynamic complex that assists in the maturation and transport of ribosomal components from the nucleus to the cytoplasm. RIOK1 plays a role in the final steps of 18S rRNA processing helping in the maturation of the 40S ribosomal subunit. This function makes it important in cellular growth and proliferation processes.
- Pathways
The kinase RIOK1/RIO1 is involved in the ribosome biogenesis pathway and the associated cell cycle progression. It interacts with several proteins like NOB1 and ENP1 which are important for small subunit maturation. The efficient function of these pathways is necessary for normal cellular function and division. These partnerships ensure the necessary quality control of new ribosome parts as they are built highlighting its critical role in maintaining cellular homeostasis.
- Associated diseases and disorders
Alterations in RIOK1/RIO1 expression have been connected to cancers and neurological disorders. Aberrant expression levels contribute to abnormal cell growth in various cancer types implicating its role in tumorigenesis. Furthermore the dysregulation of RIOK1 can influence the activity of proteins such as p53 which is an important player in managing cellular stress responses and apoptosis. This association suggests potential as a biomarker for certain oncological conditions.
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SDS-PAGE - Recombinant Human RIOK1/RIO1 protein (Tagged) (ab268939)
SDS-PAGE analysis of ab268939.
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