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AB268941

Recombinant Human RIOK3 protein (Tagged)

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Recombinant Human RIOK3 protein (Tagged) is a Human Full Length protein, in the 1 to 519 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE.

View Alternative Names

SUDD, RIOK3, Serine/threonine-protein kinase RIO3, RIO kinase 3, sudD homolog

1 Images
SDS-PAGE - Recombinant Human RIOK3 protein (Tagged) (AB268941)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human RIOK3 protein (Tagged) (AB268941)

SDS-PAGE analysis of ab268941.

Key facts

Purity

>95% SDS-PAGE

Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O14730

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MDLVGVASPEPGTAAAWGPSKCPWAIPQNTISCSLADVMSEQLAKELQLEEEAAVFPEVAVAEGPFITGENIDTSSDLMLAQMLQMEYDREYDAQLRREEKKFNGDSKVSISFENYRKVHPYEDSDSSEDEVDWQDTRDDPYRPAKPVPTPKKGFIGKGKDITTKHDEVVCGRKNTARMENFAPEFQVGDGIGMDLKLSNHVFNALKQHAYSEERRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQFFQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":519,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O14730","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIOK3 also known as RIO kinase 3 or SUDD is a serine/threonine kinase with a molecular mass of approximately 65 kDa. This protein acts as an atypical kinase within the RIO kinase family and localizes to the cytoplasm. RIOK3 shows expression in various tissues with higher levels notably in the immune system including the spleen and thymus. Unlike typical kinases RIOK3 contains a unique winged-helix domain that contributes to its diverse functions in cellular processes.
Biological function summary

RIOK3 participates in immune response regulation and cellular signal transduction. It does not form part of any known large protein complex but interacts with several other proteins through transient associations. By modulating pathways involved in immune processes RIOK3 affects the activation state of various immune cells. It is involved in the regulation of interferon production where it works closely with other signaling proteins to modulate responses to viral infections.

Pathways

RIOK3 serves a role in the innate immune response and RNA processing pathways. It influences the retinoic acid-inducible gene I (RIG-I) pathway where it interacts with proteins such as RIG-I and MAVS to mediate antiviral responses. Additionally RIOK3 plays a modulatory role in mRNA processing connecting with proteins involved in RNA maturation and internationalization which suggests its involvement in maintaining proper RNA homeostasis under stress conditions.

RIOK3 has associations with autoimmune conditions and viral infections highlighting its role in immune regulation. Aberrant functioning of RIOK3 can relate to systemic lupus erythematosus (SLE) where it influences the overproduction of interferons exacerbating the disease phenotype. Furthermore RIOK3 modulates responses to hepatitis C virus infections interacting with proteins such as RIG-I to control viral replication and immune evasion. Understanding RIOK3's functions can lead to insights into therapeutic interventions for these conditions.
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General info

Function

Serine/threonine-protein kinase involved in a ribosome quality control that takes place when ribosomes have stalled, leading to 18S non-functional rRNA decay and degradation of the 40S ribosomal subunit (PubMed : 39947182, PubMed : 39947183, PubMed : 40022732). Acts downstream of RNF10 : specifically recognizes and binds RPS2/us5 and RPS3/us3 monoubiquitinated by RNF10, promoting degradation of the 40S ribosomal subunit in a kinase-dependent manner (PubMed : 39947182, PubMed : 39947183, PubMed : 40022732). The RNF10-RIOK3 ribosome quality control takes place in response to ribosome subunit imbalance or downstream the EIF2AK4/GCN2-mediated integrated stress response (ISR) (PubMed : 39947182, PubMed : 39947183, PubMed : 40022732). Also involved in regulation of type I interferon (IFN)-dependent immune response, possibly by acting as an adapter protein essential for the recruitment of TBK1 to IRF3 (PubMed : 24807708). Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling (PubMed : 25865883). Can inhibit CASP10 isoform 7-mediated activation of the NF-kappa-B signaling pathway (PubMed : 19557502).

Sequence similarities

Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.

Post-translational modifications

Autophosphorylated (in vitro).

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Product protocols

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Target data

Serine/threonine-protein kinase involved in a ribosome quality control that takes place when ribosomes have stalled, leading to 18S non-functional rRNA decay and degradation of the 40S ribosomal subunit (PubMed : 39947182, PubMed : 39947183, PubMed : 40022732). Acts downstream of RNF10 : specifically recognizes and binds RPS2/us5 and RPS3/us3 monoubiquitinated by RNF10, promoting degradation of the 40S ribosomal subunit in a kinase-dependent manner (PubMed : 39947182, PubMed : 39947183, PubMed : 40022732). The RNF10-RIOK3 ribosome quality control takes place in response to ribosome subunit imbalance or downstream the EIF2AK4/GCN2-mediated integrated stress response (ISR) (PubMed : 39947182, PubMed : 39947183, PubMed : 40022732). Also involved in regulation of type I interferon (IFN)-dependent immune response, possibly by acting as an adapter protein essential for the recruitment of TBK1 to IRF3 (PubMed : 24807708). Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling (PubMed : 25865883). Can inhibit CASP10 isoform 7-mediated activation of the NF-kappa-B signaling pathway (PubMed : 19557502).
See full target information RIOK3
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