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AB203541

Recombinant Human RIPK4 protein

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(1 Publication)

Recombinant Human RIPK4 protein is a Human Fragment protein, in the 15 to 426 aa range, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE.

View Alternative Names

ANKRD3, DIK, RIPK4, Receptor-interacting serine/threonine-protein kinase 4, Ankyrin repeat domain-containing protein 3, PKC-delta-interacting protein kinase

1 Images
SDS-PAGE - Recombinant Human RIPK4 protein (AB203541)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human RIPK4 protein (AB203541)

Densitometric analysis of the purity of ab203541.

Key facts

Purity

>75% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P57078

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.0038% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.0017% PMSF

storage-buffer

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Complementary Products

Primary Antibodies

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Primary Antibodies

AB219620

Anti-MMP13 antibody [EPR21778]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] (AB219620)

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  • 1
Primary Antibodies

AB52918

Anti-NGF antibody [EP1320Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-NGF antibody [EP1320Y] (AB52918)

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Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"RTFDAGEFTGWEKVGSGGFGQVYKVRHVHWKTWLAIKCSPSLHVDDRERMELLEEAKKMEMAKFRYILPVYGICREPVGLVMEYMETGSLEKLLASEPLPWDLRFRIIHETAVGMNFLHCMAPPLLHLDLKPANILLDAHYHVKISDFGLAKCNGLSHSHDLSMDGLFGTIAYLPPERIREKSRLFDTKHDVYSFAIVIWGVLTQKKPFADEKNILHIMVKVVKGHRPELPPVCRARPRACSHLIRLMQRCWQGDPRVRPTFQGNGLNGELIRQVLAALLPVTGRWRSPGEGFRLESEVIIRVTCPLSSPQEITSETEDLCEKPDDEVKETAHDLDVKSPPEPRSEVVPARLKRASAPTFDNDYSLSELLSQLDSGVSQAVEGPEELSRSSSESKLPSSGSGKRLSGVSSVD","proteinLength":"Fragment","predictedMolecularWeight":"57 kDa","actualMolecularWeight":null,"aminoAcidEnd":426,"aminoAcidStart":15,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P57078","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIPK4 also known as receptor-interacting serine/threonine-protein kinase 4 plays an important role in signaling pathways. It has a molecular weight of approximately 87 kDa. RIPK4 serves in mechanistic processes by acting as a kinase that phosphorylates target proteins modulating their activity. Expression of RIPK4 predominantly occurs in epithelial cells indicating its involvement in skin structuring and possibly other epithelial-related functions.
Biological function summary

RIPK4 regulates keratinocyte differentiation and proliferation impacting skin development. This protein forms part of a protein complex that involves other signaling molecules which are important for executing its cellular functions. RIPK4's activity affects cell migration and apoptosis highlighting its importance in maintaining tissue homeostasis and integrity especially in the skin.

Pathways

RIPK4 integrates into the NF-kB and Wnt signaling pathways where it mediates cell survival and differentiation. Within the NF-kB pathway RIPK4 interacts with proteins like TRAF2 and NEMO to promote activation of downstream transcription factors. In the Wnt signaling cascade RIPK4 influences cellular responses and impacts gene expression affecting developmental and cellular processes.

Mutations or dysregulation of RIPK4 contribute to conditions such as ankyloblepharon-ectodermal defects-cleft lip/palate (AEC) syndrome and psoriasis. RIPK4 mutations link it to AEC syndrome affecting epithelial cell function and development with notable involvement of abnormalities in the TP63 protein pathway. In psoriasis altered RIPK4 expression impacts keratinocyte behavior contributing to the amplification of inflammatory pathways where this protein might interact with interleukin-related proteins.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Serine/threonine protein kinase (By similarity). Required for embryonic skin development and correct skin homeostasis in adults, via phosphorylation of PKP1 and subsequent promotion of keratinocyte differentiation and cell adhesion (By similarity). It is a direct transcriptional target of TP63 (PubMed : 22197488). Plays a role in NF-kappa-B activation (PubMed : 12446564).

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Post-translational modifications

May be phosphorylated by MAP3K2 and MAP3K3.. Proteolytically cleaved by during Fas-induced apoptosis. Cleavage at Asp-388 and Asp-426.. Polyubiquitinated with 'Lys-48' and 'Lys-63'-linked chains by BIRC2/c-IAP1 and BIRC3/c-IAP2, leading to activation of NF-kappa-B.

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Product protocols

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Target data

Serine/threonine protein kinase (By similarity). Required for embryonic skin development and correct skin homeostasis in adults, via phosphorylation of PKP1 and subsequent promotion of keratinocyte differentiation and cell adhesion (By similarity). It is a direct transcriptional target of TP63 (PubMed : 22197488). Plays a role in NF-kappa-B activation (PubMed : 12446564).
See full target information RIPK4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 12:440-449 PubMed33487072

2021

MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4.

Applications

Unspecified application

Species

Unspecified reactive species

Li Cai,Lu Ye,Xiaoqing Hu,Wenfeng He,Debao Zhuang,Qi Guo,Kuanyong Shu,Youkun Jie
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Product promise

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