Recombinant Human RKIP protein (Tag Free)
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Recombinant Human RKIP protein (Tag Free) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.
View Alternative Names
PBP, PEBP, PEBP1, Phosphatidylethanolamine-binding protein 1, PEBP-1, HCNPpp, Neuropolypeptide h3, Prostatic-binding protein, Raf kinase inhibitor protein, RKIP
- WB
Unknown
Western blot - Recombinant Human RKIP protein (Tag Free) (AB87892)
All lanes:
Western blot - Anti-RKIP antibody [EPR2875Y] (<a href='/en-us/products/primary-antibodies/rkip-antibody-epr2875y-ab76582'>ab76582</a>) at 1/5000 dilution
All lanes:
Western blot - Recombinant Human RKIP protein (Tag Free) (ab87892) at 0.1 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution
Predicted band size: 21 kDa
true
Exposure time: 30s
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human RKIP protein (Tag Free) (AB87892)
15% SDS-PAGE showing ab87892 at approximately 21kDa (3μg).
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RKIP interacts with multiple signaling pathways by acting as a suppressor of the mitogen-activated protein kinase (MAPK) pathway. It also plays roles in modulating the signaling of nuclear factor kappa B (NF-κB) and G-protein coupled receptor kinase (GRK) pathways. As part of these pathways RKIP contributes to the regulation of cell proliferation differentiation and apoptosis making it an important player in maintaining cellular homeostasis.
Pathways
RKIP's role centers on the RAF/MEK/ERK and NF-κB signaling pathways. Within the RAF/MEK/ERK pathway RKIP modulates cellular responses by controlling the activation of Raf-1 influencing cell growth and division. In the NF-κB pathway RKIP inhibits phosphorylation of IKK thereby impacting inflammatory responses which links RKIP to proteins like MEK and ERK as well as IKK.
Specifications
Form
Liquid
Additional notes
ab87892 is purified using conventional chromatography techniques.Endotoxin level: < 1.0 EU per 1 microgram of protein (determined by LAL method).
General info
Function
Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase (By similarity). Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation.. HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor (By similarity).
Sequence similarities
Belongs to the phosphatidylethanolamine-binding protein family.
Target data
Complementary Products
Product promise
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