Recombinant Human RNA polymerase II RPB4 protein (denatured) (His tag N-Terminus)
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Recombinant Human RNA polymerase II RPB4 protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 142 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.
View Alternative Names
DNA-directed RNA polymerase II subunit RPB4, RNA polymerase II subunit B4, DNA-directed RNA polymerase II subunit D, RNA polymerase II 16 kDa subunit, RPB16, POLR2D
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human RNA polymerase II RPB4 protein (denatured) (His tag N-Terminus) (AB168087)
15% SDS-PAGE analysis of ab168087 (3μg).
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RNA polymerase II RPB4 functions as a component of the larger RNA polymerase II complex. It contributes to the regulation and initiation of mRNA synthesis. This subunit has essential roles in transcriptional regulation under stress conditions by stabilizing the RNA polymerase II complex. The interaction with co-factors and other transcription machinery highlights RPB4's involvement in adaptive responses of the cell.
Pathways
RNA polymerase II RPB4 integrates into essential transcription pathways such as the mRNA processing and the transcription initiation pathway. It cooperates with other subunits and factors like TFIIB and TFIIH to effectively facilitate the transcription process. These pathways determine the accurate transcription and downstream processes necessary for gene expression playing an interconnected role with other proteins like TAFs within the transcriptional machinery.
Specifications
Form
Liquid
General info
Function
Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. POLR2D/RPB4 is part of a subcomplex with POLR2G/RPB7 that binds to a pocket formed by POLR2A/RPB1, POLR2B/RPB2 and POLR2F/RPABC2 at the base of the clamp element. The POLR2D/RPB4-POLR2G/RPB7 subcomplex seems to lock the clamp via POLR2G/RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The POLR2D/RPB4-POLR2G/RPB7 subcomplex binds single-stranded DNA and RNA.
Sequence similarities
Belongs to the eukaryotic RPB4 RNA polymerase subunit family.
Subcellular localisation
Nucleus
Target data
Complementary Products
Product promise
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