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AB160715

Recombinant Human RNF10 protein

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Recombinant Human RNF10 protein is a Human Full Length protein, in the 1 to 811 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

KIAA0262, RIE2, RNF10, E3 ubiquitin-protein ligase RNF10, RING finger protein 10

1 Images
SDS-PAGE - Recombinant Human RNF10 protein (AB160715)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RNF10 protein (AB160715)

ab160715 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q8N5U6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MPLSSPNAAATASDMDKNSGSNSSSASSGSSKGQQPPRSASAGPAGESKPKSDGKNSSGSKRYNRKRELSYPKNESFNNQSRRSSSQKSKTFNKMPPQRGGGSSKLFSSSFNGGRRDEVAEAQRAEFSPAQFSGPKKINLNHLLNFTFEPRGQTGHFEGSGHGSWGKRNKWGHKPFNKELFLQANCQFVVSEDQDYTAHFADPDTLVNWDFVEQVRICSHEVPSCPICLYPPTAAKITRCGHIFCWACILHYLSLSEKTWSKCPICYSSVHKKDLKSVVATESHQYVVGDTITMQLMKREKGVLVALPKSKWMNVDHPIHLGDEQHSQYSKLLLASKEQVLHRVVLEEKVALEQQLAEEKHTPESCFIEAAIQELKTREEALSGLAGSRREVTGVVAALEQLVLMAPLAKESVFQPRKGVLEYLSAFDEETTEVCSLDTPSRPLALPLVEEEEAVSEPEPEGLPEACDDLELADDNLKEGTICTESSQQEPITKSGFTRLSSSPCYYFYQAEDGQHMFLHPVNVRCLVREYGSLERSPEKISATVVEIAGYSMSEDVRQRHRYLSHLPLTCEFSICELALQPPVVSKETLEMFSDDIEKRKRQRQKKAREERRRERRIEIEENKKQGKYPEVHIPLENLQQFPAFNSYTCSSDSALGPTSTEGHGALSISPLSRSPGSHADFLLTPLSPTASQGSPSFCVGSLEEDSPFPSFAQMLRVGKAKADVWPKTAPKKDENSLVPPAPVDSDGESDNSDRVPVPSFQNSFSQAIEAAFMKLDTPATSDPLSEEKGGKKRKKQKQKLLFSTSVVHTK","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":811,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q8N5U6","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF10 also known as RING finger protein 10 is an E3 ubiquitin-protein ligase that plays a role in attaching ubiquitin molecules to target substrates marking them for proteasomal degradation. RNF10 has a molecular weight of approximately 75 kDa. It is expressed in various tissues including the brain heart and skeletal muscle. RNF10 contains a RING finger domain important for its ligase activity allowing it to interact with E2 ubiquitin-conjugating enzymes.
Biological function summary

RNF10 participates in regulating cellular processes like neurodevelopment and muscle differentiation. It interacts with other proteins to form complexes that influence cell signaling pathways and transcription regulation. This protein's activities affect neuronal growth and synaptic plasticity important for learning and memory. RNF10 also has implications in muscle cells where it modulates the expression of genes related to muscle function.

Pathways

RNF10 takes part in the MAPK signaling pathway which plays a role in cell proliferation and differentiation. In this pathway RNF10 interacts with proteins such as MEK and ERK allowing it to influence downstream signaling processes. Additionally RNF10 has connections to the Wnt signaling pathway impacting gene expression patterns important for development. These interactions highlight its role in orchestrating complex cellular responses.

RNF10 is linked to neurological and muscular conditions. Its involvement in neurodevelopment suggests a connection to neurodegenerative diseases like Alzheimer's where protein degradation processes may go awry. In muscular disorders RNF10 may interact with proteins like MYOD1 influencing muscle differentiation and regeneration. These associations highlight the importance of understanding RNF10's role in healthy and diseased states.
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Specifications

Form

Liquid

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General info

Function

E3 ubiquitin-protein ligase that catalyzes monoubiquitination of 40S ribosomal proteins RPS2/us5 and RPS3/us3 in response to ribosome stalling (PubMed : 34348161, PubMed : 34469731). Part of a ribosome quality control that takes place when ribosomes have stalled during translation initiation (iRQC) : RNF10 acts by mediating monoubiquitination of RPS2/us5 and RPS3/us3, promoting their degradation by the proteasome (PubMed : 34348161, PubMed : 34469731). Also promotes ubiquitination of 40S ribosomal proteins in response to ribosome stalling during translation elongation (PubMed : 34348161). The action of RNF10 in iRQC is counteracted by USP10 (PubMed : 34469731). May also act as a transcriptional factor involved in the regulation of MAG (Myelin-associated glycoprotein) expression (By similarity). Acts as a regulator of Schwann cell differentiation and myelination (By similarity).

Sequence similarities

Belongs to the RNF10 family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

E3 ubiquitin-protein ligase that catalyzes monoubiquitination of 40S ribosomal proteins RPS2/us5 and RPS3/us3 in response to ribosome stalling (PubMed : 34348161, PubMed : 34469731). Part of a ribosome quality control that takes place when ribosomes have stalled during translation initiation (iRQC) : RNF10 acts by mediating monoubiquitination of RPS2/us5 and RPS3/us3, promoting their degradation by the proteasome (PubMed : 34348161, PubMed : 34469731). Also promotes ubiquitination of 40S ribosomal proteins in response to ribosome stalling during translation elongation (PubMed : 34348161). The action of RNF10 in iRQC is counteracted by USP10 (PubMed : 34469731). May also act as a transcriptional factor involved in the regulation of MAG (Myelin-associated glycoprotein) expression (By similarity). Acts as a regulator of Schwann cell differentiation and myelination (By similarity).
See full target information RNF10
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