Recombinant Human RNF121 protein is a Human Fragment protein, in the 193 to 301 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Publications
Filter by
- PLoS pathogens 15:e10079882019Ring finger protein 121 is a potent regulator of adeno-associated viral genome transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVictoria J Madigan et. al.PubMed 31386698
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
E R D F A E M C A D Y M A S T I G F Y S E S G M P T K H L S D S V C A V C G Q Q I F V D V S E E G I I E N T Y R L S C N H V F H E F C I R G W C I V G K K Q T C P Y C K E K V D L K R M F S N P W E R P H V M Y G Q L L D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
E3 ubiquitin ligase which accepts ubiquitin and transfers it to substrates thereby promoting their degradation by the endoplasmic reticulum-associated degradation (ERAD) pathway which is a pathway involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (By similarity). May regulate the unfolded protein response to reduce endoplasmic reticulum stress (By similarity).
Alternative names
E3 ubiquitin ligase RNF121, RING finger protein 121, RNF121
Recommended products
Recombinant Human RNF121 protein is a Human Fragment protein, in the 193 to 301 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9H920-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- E R D F A E M C A D Y M A S T I G F Y S E S G M P T K H L S D S V C A V C G Q Q I F V D V S E E G I I E N T Y R L S C N H V F H E F C I R G W C I V G K K Q T C P Y C K E K V D L K R M F S N P W E R P H V M Y G Q L L D
- Accession
- Q9H920
- Protein length
- Fragment
- Amino acids
- 193 to 301
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
E3 ubiquitin ligase which accepts ubiquitin and transfers it to substrates thereby promoting their degradation by the endoplasmic reticulum-associated degradation (ERAD) pathway which is a pathway involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (By similarity). May regulate the unfolded protein response to reduce endoplasmic reticulum stress (By similarity).
- Sequence similarities
Belongs to the RNF121 family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RNF121 also known as Ring Finger Protein 121 is a protein that functions mechanistically as an E3 ubiquitin ligase. This enzyme catalyzes the transfer of ubiquitin from an E2 ubiquitin-conjugating enzyme to a substrate protein tagging it for degradation by the proteasome. RNF121 has a molecular mass of approximately 44 kDa. This protein is widely expressed in the cytoplasm and is abundant in tissues such as the heart brain liver and kidneys amongst others indicating its diverse role in cellular function.
- Biological function summary
RNF121 contributes to processes such as protein homeostasis and regulation of signaling pathways through its involvement in the ubiquitin-proteasome system. It does not function alone; RNF121 often associates with other cellular proteins to form complexes that target specific proteins for ubiquitination. This process is integral for controlling the degradation of damaged or misfolded proteins and for regulating diverse signaling pathways within the cell.
- Pathways
The ubiquitin ligase activity of RNF121 plays roles in the Wnt signaling pathway and endoplasmic reticulum-associated degradation (ERAD) pathway. In the Wnt pathway RNF121 interacts with proteins like beta-catenin modulating its stability and consequently influencing gene expression related to cell growth and differentiation. In the ERAD pathway RNF121 helps manage protein quality control by targeting misfolded proteins for degradation ensuring cellular health and function.
- Associated diseases and disorders
RNF121 has been linked to cancer and neurodegenerative diseases. Mutations or dysregulation of RNF121 can influence the stability of key regulatory proteins leading to unregulated cellular proliferation associated with tumor progression. Similarly alterations in RNF121 might disrupt protein degradation processes contributing to the accumulation of misfolded proteins observed in neurodegenerative conditions. Connections between RNF121 and proteins involved in these diseases such as p53 in cancer or tau in Alzheimer’s disease highlight its role in disease mechanisms.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human RNF121 protein (ab163056)
ab163056 on a 12.5% SDS-PAGE stained with Coomassie Blue.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com