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AB153534

Recombinant Human RNF146 protein

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Recombinant Human RNF146 protein is a Human Full Length protein, in the 1 to 358 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

E3 ubiquitin-protein ligase RNF146, Dactylidin, Iduna, RING finger protein 146, RING-type E3 ubiquitin transferase RNF146, RNF146

1 Images
SDS-PAGE - Recombinant Human RNF146 protein (AB153534)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RNF146 protein (AB153534)

ab153534 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q9NTX7

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MAGCGEIDHSINMLPTNRKANESCSNTAPSLTVPECAICLQTCVHPVSLPCKHVFCYLCVKGASWLGKRCALCRQEIPEDFLDKPTLLSPEELKAASRGNGEYAWYYEGRNGWWQYDERTSRELEDAFSKGKKNTEMLIAGFLYVADLENMVQYRRNEHGRRRKIKRDIIDIPKKGVAGLRLDCDANTVNLARESSADGADSVSAQSGASVQPLVSSVRPLTSVDGQLTSPATPSPDASTSLEDSFAHLQLSGDNTAERSHRGEGEEDHESPSSGRVPAPDTSIEETESDASSDSEDVSAVVAQHSLTQQRLLVSNANQTVPDRSDRSGTDRSVAGGGTVSVSVRSRRPDGQCTVTEV","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":358,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9NTX7","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF146 also known as Iduna or dactylidin is an E3 ubiquitin ligase with a molecular mass of approximately 33 kDa. It plays an important role in the poly(ADP-ribose) (PAR)-dependent ubiquitination process adding ubiquitin to target proteins for degradation. RNF146 binds to PAR chains using its WWE domain triggering the ubiquitin-proteasome pathway. This protein is widely expressed in tissues notably in the brain heart and testis indicating its involvement in various cellular activities.
Biological function summary

RNF146 exhibits significant functions that influence cell survival and DNA repair. It interacts with PARP1 an important protein in the DNA damage response. As part of a larger protein complex RNF146 identifies and ubiquitinates proteins modified by PAR facilitating their recognition and subsequent degradation by the proteasome. Through these interactions RNF146 controls levels of damaged proteins and modulates the cellular response to stress contributing to genomic stability.

Pathways

RNF146 plays a part in the Wnt/β-catenin signaling pathway and the DNA damage response pathway. Within the Wnt signaling pathway RNF146 regulates Axin1 and other components influencing cell proliferation and differentiation. Additionally in the DNA damage pathway RNF146 aids in the fine-tuning of protein levels necessary for efficient repair by controlling PAR-modified proteins. Its interactions with proteins such as PARP1 and Tankyrase position RNF146 as a significant player in these pathways.

RNF146 has connections to neurodegenerative diseases and cancer. Abnormal RNF146 expression or activity can disrupt cellular homeostasis contributing to the pathogenesis of Alzheimer's disease through interactions with proteins like Tankyrase. In cancer altered RNF146 activity can influence tumorigenesis by modulating key signaling pathways such as the Wnt/β-catenin pathway. These links suggest that targeting RNF146 could offer therapeutic potential for both neurodegenerative diseases and cancer.
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Specifications

Form

Liquid

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General info

Function

E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation (PubMed : 21478859, PubMed : 21799911, PubMed : 22267412). May regulate many important biological processes, such as cell survival and DNA damage response (PubMed : 21825151, PubMed : 22267412). Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex (PubMed : 21478859, PubMed : 21799911). Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2 (PubMed : 21799911). Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination, leading to their degradation (PubMed : 21799911). Different ubiquitin linkage types have been observed : TNKS2 undergoes ubiquitination at 'Lys-48' and 'Lys-63', while AXIN1 is only ubiquitinated at 'Lys-48' (PubMed : 21799911). May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location (PubMed : 21799911). Neuroprotective protein (PubMed : 21602803). Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos (By similarity). Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner (By similarity). Does not affect PARP1 activation (By similarity). Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest (By similarity). Promotes cell survival after gamma-irradiation (PubMed : 21825151). Facilitates DNA repair (PubMed : 21825151).

Post-translational modifications

Ubiquitinated; autoubiquitinated. Polyubiquitinated in the presence of UBE2D1, UBE2D2 and UBE2D3. Multimonoubiquitinated in the presence of UBE2E1. Not ubiquitinated in the presence of UBE2H, CDC34, UBE2L3, UBE2L6, nor UBE2C. In the absence of PAR, autoubiquitination occurs on Lys-85, Lys-95 and Lys-176 via 'Lys-11' and 'Lys-48' ubiquitin linkages. In the presence of PAR, Lys-131 and Lys-176 are ubiquitinated via 'Lys-6', 'Lys-33' and 'Lys-48' ubiquitin linkages. Autoubiquitination is enhanced upon PAR-binding.

Subcellular localisation

Nucleus

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Product protocols

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Target data

E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation (PubMed : 21478859, PubMed : 21799911, PubMed : 22267412). May regulate many important biological processes, such as cell survival and DNA damage response (PubMed : 21825151, PubMed : 22267412). Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex (PubMed : 21478859, PubMed : 21799911). Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2 (PubMed : 21799911). Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination, leading to their degradation (PubMed : 21799911). Different ubiquitin linkage types have been observed : TNKS2 undergoes ubiquitination at 'Lys-48' and 'Lys-63', while AXIN1 is only ubiquitinated at 'Lys-48' (PubMed : 21799911). May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location (PubMed : 21799911). Neuroprotective protein (PubMed : 21602803). Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos (By similarity). Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner (By similarity). Does not affect PARP1 activation (By similarity). Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest (By similarity). Promotes cell survival after gamma-irradiation (PubMed : 21825151). Facilitates DNA repair (PubMed : 21825151).
See full target information E3 ubiquitin-protein ligase RNF146
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Complementary Products

Primary Antibodies

AB201212

Anti-RNF146 antibody

Western blot - Anti-RNF146 antibody (AB201212)

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