JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB153613

Recombinant Human RNF168 protein (GST tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human RNF168 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 571 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

E3 ubiquitin-protein ligase RNF168, hRNF168, RING finger protein 168, RING-type E3 ubiquitin transferase RNF168, RNF168

1 Images
SDS-PAGE - Recombinant Human RNF168 protein (GST tag N-Terminus) (AB153613)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RNF168 protein (GST tag N-Terminus) (AB153613)

ab153613 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q8IYW5

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

style
left-column

Complementary Products

Primary Antibodies

AB303619

Anti-RNF168 antibody [EPR24239-2] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-RNF168 antibody [EPR24239-2] - BSA and Azide free (Detector) (AB303619)

  • 0
  • 0
Proteins & Peptides

AB198464

Recombinant human HAUSP / USP7 protein (His-DDDDK tag N-Terminus)

Functional Studies - Recombinant human HAUSP / USP7 protein (His-DDDDK tag N-Terminus) (AB198464)

  • 0
  • 0
Proteins & Peptides

AB165455

Recombinant Human ZNF358 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ZNF358 protein (GST tag N-Terminus) (AB165455)

  • 0
  • 0
Proteins & Peptides

AB313361

Recombinant Human WFDC2 Protein (His tag)

Mass Spectrometry - Recombinant Human WFDC2 Protein (His tag) (AB313361)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MALPKDAIPSLSECQCGICMEILVEPVTLPCNHTLCKPCFQSTVEKASLCCPFCRRRVSSWTRYHTRRNSLVNVELWTIIQKHYPRECKLRASGQESEEVADDYQPVRLLSKPGELRREYEEEISKVAAERRASEEEENKASEEYIQRLLAEEEEEEKRQAEKRRRAMEEQLKSDEELARKLSIDINNFCEGSISASPLNSRKSDPVTPKSEKKSKNKQRNTGDIQKYLTPKSQFGSASHSEAVQEVRKDSVSKDIDSSDRKSPTGQDTEIEDMPTLSPQISLGVGEQGADSSIESPMPWLCACGAEWYHEGNVKTRPSNHGKELCVLSHERPKTRVPYSKETAVMPCGRTESGCAPTSGVTQTNGNNTGETENEESCLLISKEISKRKNQESSFEAVKDPCFSAKRRKVSPESSPDQEETEINFTQKLIDLEHLLFERHKQEEQDRLLALQLQKEVDKEQMVPNRQKGSPDEYHLRATSSPPDKVLNGQRKNPKDGNFKRQTHTKHPTPERGSRDKNRQVSLKMQLKQSVNRRKMPNSTRDHCKVSKSAHSLQPSISQKSVFQMFQRCTK","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":571,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q8IYW5","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF168 also known as Ring Finger Protein 168 is an E3 ubiquitin ligase with a molecular mass of approximately 63 kDa. It functions primarily in the DNA damage response by adding ubiquitin to histones at sites of double-strand breaks. RNF168 is expressed in various tissues but is notably active in cells that experience DNA damage. This protein structure contains a RING finger domain which facilitates its role in ubiquitination.
Biological function summary

The actions of RNF168 are critical in maintaining genome stability and it serves as a part of the DNA damage signaling complex. It collaborates with other proteins in recognizing DNA damage and signaling repair processes. RNF168 amplifies the ubiquitin signal which then attracts repair proteins to the site of damage. This function places it as an important component in the cellular response to DNA breaks.

Pathways

RNF168 is an important player in the DNA damage response pathway and is particularly associated with the homologous recombination repair mechanism. It works alongside proteins such as 53BP1 and BRCA1 to initiate repair processes. RNF168’s ubiquitination activity facilitates the recruitment of these repair proteins coordinating the cellular response to DNA lesions and ensuring effective repair.

RNF168 mutations or malfunctions have been linked to conditions like RIDDLE syndrome and certain cancers. In RIDDLE syndrome deficiencies in RNF168 disrupt the normal DNA repair processes leading to immunodeficiency radiosensitivity and learning difficulties. Cancer arises when the impaired DNA damage response fails to prevent mutations. Understanding RNF168 interactions particularly with proteins like 53BP1 and BRCA1 is essential for developing therapeutic strategies for these conditions.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8-dependent H2A ubiquitination, promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. Following DNA damage, promotes the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF8, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Not able to initiate 'Lys-63'-linked ubiquitination in vitro; possibly due to partial occlusion of the UBE2N/UBC13-binding region. Catalyzes monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively).

Sequence similarities

Belongs to the RNF168 family.

Post-translational modifications

Sumoylated with SUMO1 by PIAS4 in response to double-strand breaks (DSBs).. Ubiquitinated.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8-dependent H2A ubiquitination, promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. Following DNA damage, promotes the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF8, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Not able to initiate 'Lys-63'-linked ubiquitination in vitro; possibly due to partial occlusion of the UBE2N/UBC13-binding region. Catalyzes monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively).
See full target information RNF168
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com