Recombinant Human RNF168 protein is a Human Full Length protein, in the 1 to 571 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M A L P K D A I P S L S E C Q C G I C M E I L V E P V T L P C N H T L C K P C F Q S T V E K A S L C C P F C R R R V S S W T R Y H T R R N S L V N V E L W T I I Q K H Y P R E C K L R A S G Q E S E E V A D D Y Q P V R L L S K P G E L R R E Y E E E I S K V A A E R R A S E E E E N K A S E E Y I Q R L L A E E E E E E K R Q A E K R R R A M E E Q L K S D E E L A R K L S I D I N N F C E G S I S A S P L N S R K S D P V T P K S E K K S K N K Q R N T G D I Q K Y L T P K S Q F G S A S H S E A V Q E V R K D S V S K D I D S S D R K S P T G Q D T E I E D M P T L S P Q I S L G V G E Q G A D S S I E S P M P W L C A C G A E W Y H E G N V K T R P S N H G K E L C V L S H E R P K T R V P Y S K E T A V M P C G R T E S G C A P T S G V T Q T N G N N T G E T E N E E S C L L I S K E I S K R K N Q E S S F E A V K D P C F S A K R R K V S P E S S P D Q E E T E I N F T Q K L I D L E H L L F E R H K Q E E Q D R L L A L Q L Q K E V D K E Q M V P N R Q K G S P D E Y H L R A T S S P P D K V L N G Q R K N P K D G N F K R Q T H T K H P T P E R G S R D K N R Q V S L K M Q L K Q S V N R R K M P N S T R D H C K V S K S A H S L Q P S I S Q K S V F Q M F Q R C T K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8-dependent H2A ubiquitination, promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. Following DNA damage, promotes the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF8, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Not able to initiate 'Lys-63'-linked ubiquitination in vitro; possibly due to partial occlusion of the UBE2N/UBC13-binding region. Catalyzes monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively).
Alternative names
E3 ubiquitin-protein ligase RNF168, hRNF168, RING finger protein 168, RING-type E3 ubiquitin transferase RNF168, RNF168
Recommended products
Recombinant Human RNF168 protein is a Human Full Length protein, in the 1 to 571 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q8IYW5-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A L P K D A I P S L S E C Q C G I C M E I L V E P V T L P C N H T L C K P C F Q S T V E K A S L C C P F C R R R V S S W T R Y H T R R N S L V N V E L W T I I Q K H Y P R E C K L R A S G Q E S E E V A D D Y Q P V R L L S K P G E L R R E Y E E E I S K V A A E R R A S E E E E N K A S E E Y I Q R L L A E E E E E E K R Q A E K R R R A M E E Q L K S D E E L A R K L S I D I N N F C E G S I S A S P L N S R K S D P V T P K S E K K S K N K Q R N T G D I Q K Y L T P K S Q F G S A S H S E A V Q E V R K D S V S K D I D S S D R K S P T G Q D T E I E D M P T L S P Q I S L G V G E Q G A D S S I E S P M P W L C A C G A E W Y H E G N V K T R P S N H G K E L C V L S H E R P K T R V P Y S K E T A V M P C G R T E S G C A P T S G V T Q T N G N N T G E T E N E E S C L L I S K E I S K R K N Q E S S F E A V K D P C F S A K R R K V S P E S S P D Q E E T E I N F T Q K L I D L E H L L F E R H K Q E E Q D R L L A L Q L Q K E V D K E Q M V P N R Q K G S P D E Y H L R A T S S P P D K V L N G Q R K N P K D G N F K R Q T H T K H P T P E R G S R D K N R Q V S L K M Q L K Q S V N R R K M P N S T R D H C K V S K S A H S L Q P S I S Q K S V F Q M F Q R C T K
- Accession
- Q8IYW5
- Protein length
- Full Length
- Amino acids
- 1 to 571
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8-dependent H2A ubiquitination, promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. Following DNA damage, promotes the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF8, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Not able to initiate 'Lys-63'-linked ubiquitination in vitro; possibly due to partial occlusion of the UBE2N/UBC13-binding region. Catalyzes monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively).
- Sequence similarities
Belongs to the RNF168 family.
- Post-translational modifications
Sumoylated with SUMO1 by PIAS4 in response to double-strand breaks (DSBs).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RNF168 also known as Ring Finger Protein 168 is an E3 ubiquitin ligase with a molecular mass of approximately 63 kDa. It functions primarily in the DNA damage response by adding ubiquitin to histones at sites of double-strand breaks. RNF168 is expressed in various tissues but is notably active in cells that experience DNA damage. This protein structure contains a RING finger domain which facilitates its role in ubiquitination.
- Biological function summary
The actions of RNF168 are critical in maintaining genome stability and it serves as a part of the DNA damage signaling complex. It collaborates with other proteins in recognizing DNA damage and signaling repair processes. RNF168 amplifies the ubiquitin signal which then attracts repair proteins to the site of damage. This function places it as an important component in the cellular response to DNA breaks.
- Pathways
RNF168 is an important player in the DNA damage response pathway and is particularly associated with the homologous recombination repair mechanism. It works alongside proteins such as 53BP1 and BRCA1 to initiate repair processes. RNF168’s ubiquitination activity facilitates the recruitment of these repair proteins coordinating the cellular response to DNA lesions and ensuring effective repair.
- Associated diseases and disorders
RNF168 mutations or malfunctions have been linked to conditions like RIDDLE syndrome and certain cancers. In RIDDLE syndrome deficiencies in RNF168 disrupt the normal DNA repair processes leading to immunodeficiency radiosensitivity and learning difficulties. Cancer arises when the impaired DNA damage response fails to prevent mutations. Understanding RNF168 interactions particularly with proteins like 53BP1 and BRCA1 is essential for developing therapeutic strategies for these conditions.
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1 product image
SDS-PAGE - Recombinant Human RNF168 protein (ab153613)
ab153613 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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