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AB162777

Recombinant Human RNF186 protein (GST tag N-Terminus)

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Recombinant Human RNF186 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 227 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

E3 ubiquitin-protein ligase RNF186, RING finger protein 186, RNF186

1 Images
SDS-PAGE - Recombinant Human RNF186 protein (GST tag N-Terminus) (AB162777)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RNF186 protein (GST tag N-Terminus) (AB162777)

ab162777 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q9NXI6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MACTKTLQQSQPISAGATTTTTAVAPAGGHSGSTECDLECLVCREPYSCPRLPKLLACQHAFCAICLKLLLCVQDNTWSITCPLCRKVTAVPGGLICSLRDHEAVVGQLAQPCTEVSLCPQGLVDPADLAAGHPSLVGEDGQDEVSANHVAARRLAAHLLLLALLIILIGPFIYPGVLRWVLTFIIALALLMSTLFCCLPSTRGSCWPSSRTLFCREQKHSHISSIA","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":227,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9NXI6","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF186 also known as Ring Finger Protein 186 is a small protein with a mass of approximately 22 kDa. It contains a RING finger domain categorizing it as an E3 ubiquitin ligase. This protein facilitates ubiquitination by transferring ubiquitin molecules from E2 ubiquitin-conjugating enzymes to substrate proteins. RNF186 is mainly expressed in tissues such as the pancreas small intestine and colon. By modifying the stability and signaling activity of target proteins it plays a role in protein homeostasis.
Biological function summary

The modulation of protein ubiquitination by RNF186 influences cellular stress responses. It is part of a larger network concerning cellular homeostasis and response to stress although not necessarily functioning in a discrete large multiprotein complex. Through these activities RNF186 helps maintain cellular health particularly under stress conditions that involve protein misfolding or cellular damage in the gastrointestinal tract.

Pathways

The role of RNF186 in maintaining cell homeostasis links it to the unfolded protein response (UPR) and autophagy pathways. The protein assists in mediating stress-related signaling that occurs during UPR which is vital in conditions of endoplasmic reticulum stress. It also plays a part in autophagy regulation directing damaged proteins and organelles for degradation. RNF186 interacts with other proteins such as BECN1 which is important for autophagosome formation and function.

The dysregulation of RNF186 has connections to inflammatory bowel disease (IBD) and chronic pancreatitis. In IBD RNF186 interacts with proteins like NOD2 a known player in immune response regulation and inflammation. Mutations or altered function of RNF186 can impair its role in protein degradation and stress response exacerbating inflammation and tissue damage in the gastrointestinal tract. In chronic pancreatitis the involvement of RNF186 in stress response pathways suggests its potential impact on pancreatic tissue integrity and function under chronic stress conditions.
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Specifications

Form

Liquid

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General info

Function

E3 ubiquitin protein ligase that is part of an apoptotic signaling pathway activated by endoplasmic reticulum stress (PubMed : 23896122). Stimulates the expression of proteins specific of the unfolded protein response (UPR), ubiquitinates BNIP1 and regulates its localization to the mitochondrion and induces calcium release from the endoplasmic reticulum that ultimately leads to cell apoptosis (PubMed : 23896122). Plays a role in the maintenance of intestinal homeostasis and clearance of enteric pathogens. Upon NOD2 stimulation, ubiquitinates the ER stress sensor activating transcription factor 6/ATF6 and promotes the unfolded protein response UPR (PubMed : 34623328). Participates in basal level of autophagy maintenance by regulating the ubiquitination of EPHB2 and EPHB3. Upon stimulation by ligand EFNB1, ubiquitinates EPHB2 and further recruits MAP1LC3B for autophagy induction (PubMed : 33280498). Controls nutrient sensing by ubiquitinating Sestrin-2/SESN2, which is an intracellular sensor of cytosolic leucine and inhibitor of mTORC1 activity (PubMed : 31586034).

Post-translational modifications

Polyubiquitinated. 'Lys-29'-linked autoubiquitination leads to proteasomal degradation.

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Product protocols

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Target data

E3 ubiquitin protein ligase that is part of an apoptotic signaling pathway activated by endoplasmic reticulum stress (PubMed : 23896122). Stimulates the expression of proteins specific of the unfolded protein response (UPR), ubiquitinates BNIP1 and regulates its localization to the mitochondrion and induces calcium release from the endoplasmic reticulum that ultimately leads to cell apoptosis (PubMed : 23896122). Plays a role in the maintenance of intestinal homeostasis and clearance of enteric pathogens. Upon NOD2 stimulation, ubiquitinates the ER stress sensor activating transcription factor 6/ATF6 and promotes the unfolded protein response UPR (PubMed : 34623328). Participates in basal level of autophagy maintenance by regulating the ubiquitination of EPHB2 and EPHB3. Upon stimulation by ligand EFNB1, ubiquitinates EPHB2 and further recruits MAP1LC3B for autophagy induction (PubMed : 33280498). Controls nutrient sensing by ubiquitinating Sestrin-2/SESN2, which is an intracellular sensor of cytosolic leucine and inhibitor of mTORC1 activity (PubMed : 31586034).
See full target information RNF186
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