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Recombinant Human RNF4 protein is a Human Full Length protein, in the 1 to 190 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.

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Images

SDS-PAGE - Recombinant Human RNF4 protein (AB134611), expandable thumbnail

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, MS

Biologically active

No

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M G S M S T R K R R G G A I N S R Q A Q K R T R E A T S T P E I S L E A E P I E L V E T A G D E I V D L T C E S L E P V V V D L T H N D S V V I V D E R R R P R R N A R R L P Q D H A D S C V V S S D D E E L S R D R D V Y V T T H T P R N A R D E G A T G L R P S G T V S C P I C M D G Y S E I V Q N G R L I V S T E C G H V F C S Q C L R D S L K N A N T C P T C R K K I N H K R Y H P I Y I

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation (PubMed:18408734, PubMed:19307308, PubMed:35013556). Regulates the degradation of several proteins including PML and the transcriptional activator PEA3 (PubMed:18408734, PubMed:19307308, PubMed:20943951). Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation (PubMed:20212317). Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 (PubMed:19779455, PubMed:20026589). Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation (PubMed:12885770). Catalyzes ubiquitination of sumoylated PARP1 in response to PARP1 trapping to chromatin, leading to PARP1 removal from chromatin by VCP/p97 (PubMed:35013556).

Alternative names

Recommended products

Recombinant Human RNF4 protein is a Human Full Length protein, in the 1 to 190 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, MS

Accession
P78317-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Sequence info

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M G S M S T R K R R G G A I N S R Q A Q K R T R E A T S T P E I S L E A E P I E L V E T A G D E I V D L T C E S L E P V V V D L T H N D S V V I V D E R R R P R R N A R R L P Q D H A D S C V V S S D D E E L S R D R D V Y V T T H T P R N A R D E G A T G L R P S G T V S C P I C M D G Y S E I V Q N G R L I V S T E C G H V F C S Q C L R D S L K N A N T C P T C R K K I N H K R Y H P I Y I

Accession

P78317

Protein length

Full Length

Predicted molecular weight

23.7 kDa

Amino acids

1 to 190

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

Additional notes

ab134611 was purified using conventional chromatography techniques.

General info

Function

E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation (PubMed:18408734, PubMed:19307308, PubMed:35013556). Regulates the degradation of several proteins including PML and the transcriptional activator PEA3 (PubMed:18408734, PubMed:19307308, PubMed:20943951). Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation (PubMed:20212317). Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 (PubMed:19779455, PubMed:20026589). Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation (PubMed:12885770). Catalyzes ubiquitination of sumoylated PARP1 in response to PARP1 trapping to chromatin, leading to PARP1 removal from chromatin by VCP/p97 (PubMed:35013556).

Post-translational modifications

Sumoylated; conjugated by one or two SUMO1 moieties.

Subcellular localisation

Nucleus, PML body, Nucleoplasm

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • SDS-PAGE - Recombinant Human RNF4 protein (ab134611), expandable thumbnail

    SDS-PAGE - Recombinant Human RNF4 protein (ab134611)

    15% SDS-PAGE analysis of ab134611 (3μg).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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