Recombinant Human RNF4 protein is a Human Full Length protein, in the 1 to 190 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
>90% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE, MS
No
M G S S H H H H H H S S G L V P R G S H M G S M S T R K R R G G A I N S R Q A Q K R T R E A T S T P E I S L E A E P I E L V E T A G D E I V D L T C E S L E P V V V D L T H N D S V V I V D E R R R P R R N A R R L P Q D H A D S C V V S S D D E E L S R D R D V Y V T T H T P R N A R D E G A T G L R P S G T V S C P I C M D G Y S E I V Q N G R L I V S T E C G H V F C S Q C L R D S L K N A N T C P T C R K K I N H K R Y H P I Y I
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation (PubMed:18408734, PubMed:19307308, PubMed:35013556). Regulates the degradation of several proteins including PML and the transcriptional activator PEA3 (PubMed:18408734, PubMed:19307308, PubMed:20943951). Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation (PubMed:20212317). Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 (PubMed:19779455, PubMed:20026589). Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation (PubMed:12885770). Catalyzes ubiquitination of sumoylated PARP1 in response to PARP1 trapping to chromatin, leading to PARP1 removal from chromatin by VCP/p97 (PubMed:35013556).
SNURF, RES4-26, RNF4, SNURF, RES4-26, E3 ubiquitin-protein ligase RNF4, RING finger protein 4, Small nuclear ring finger protein, Protein SNURF
Recombinant Human RNF4 protein is a Human Full Length protein, in the 1 to 190 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
>90% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE, MS
No
No
Human
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
M G S S H H H H H H S S G L V P R G S H M G S M S T R K R R G G A I N S R Q A Q K R T R E A T S T P E I S L E A E P I E L V E T A G D E I V D L T C E S L E P V V V D L T H N D S V V I V D E R R R P R R N A R R L P Q D H A D S C V V S S D D E E L S R D R D V Y V T T H T P R N A R D E G A T G L R P S G T V S C P I C M D G Y S E I V Q N G R L I V S T E C G H V F C S Q C L R D S L K N A N T C P T C R K K I N H K R Y H P I Y I
Full Length
23.7 kDa
1 to 190
Recombinant
His tag N-Terminus
Liquid
ab134611 was purified using conventional chromatography techniques.
E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation (PubMed:18408734, PubMed:19307308, PubMed:35013556). Regulates the degradation of several proteins including PML and the transcriptional activator PEA3 (PubMed:18408734, PubMed:19307308, PubMed:20943951). Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation (PubMed:20212317). Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 (PubMed:19779455, PubMed:20026589). Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation (PubMed:12885770). Catalyzes ubiquitination of sumoylated PARP1 in response to PARP1 trapping to chromatin, leading to PARP1 removal from chromatin by VCP/p97 (PubMed:35013556).
Sumoylated; conjugated by one or two SUMO1 moieties.
Nucleus, PML body, Nucleoplasm
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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15% SDS-PAGE analysis of ab134611 (3μg).
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