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AB276721

Recombinant human RNF43 protein (Fc Chimera)

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Recombinant human RNF43 protein (Fc Chimera) is a Human Fragment protein, in the 1 to 197 aa range, expressed in HEK 293 cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

E3 ubiquitin-protein ligase RNF43, RING finger protein 43, RING-type E3 ubiquitin transferase RNF43, RNF43

2 Images
Functional Studies - Recombinant human RNF43 protein (Fc Chimera) (AB276721)
  • FuncS

Supplier Data

Functional Studies - Recombinant human RNF43 protein (Fc Chimera) (AB276721)

Measured by its binding ability in a functional ELISA. Immobilized human RSPO3-His (1-146) at 10 μg/mL (100 μL/well) can bind human RNF43-Fc. The EC50 of human RNF43-Fc is 0.01-0.03 μg/mL.

SDS-PAGE - Recombinant human RNF43 protein (Fc Chimera) (AB276721)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human RNF43 protein (Fc Chimera) (AB276721)

SDS-PAGE analysis of ab276721

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

Fc tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Measured by its binding ability in a functional ELISA. Immobilized human RSPO3-His (1-146) at 10 μg/mL (100 μL/well) can bind human RNF43-Fc. The EC50 of human RNF43-Fc is 0.01-0.03 μg/mL.

Accession

Q68DV7

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 100% PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MSGGHQLQLAALWPWLLMATLQAGFGRTGLVLAAAVESERSAEQKAIIRVIPLKMDPTGKLNLTLEGVFAGVAEITPAEGKLMQSHPLYLCNASDDDNLEPGFISIVKLESPRRAPRPCLSLASKARMAGERGASAVLFDITEDRAAAEQLQQPLGLTWPVVLIWGNDAEKLMEFVYKNQKAHVRIELKEPPAWPDY","proteinLength":"Fragment","predictedMolecularWeight":"45.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":197,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q68DV7","tags":[{"tag":"Fc","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Lyophilized

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General info

Function

E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway (PubMed : 18313049, PubMed : 22575959, PubMed : 22895187). Along with RSPO2 and ZNRF3, constitutes a master switch that governs limb specification (By similarity).

Sequence similarities

Belongs to the ZNRF3 family.

Post-translational modifications

Autoubiquitinated.

Subcellular localisation

Nucleus envelope

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Product protocols

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Target data

E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway (PubMed : 18313049, PubMed : 22575959, PubMed : 22895187). Along with RSPO2 and ZNRF3, constitutes a master switch that governs limb specification (By similarity).
See full target information RNF43
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