Recombinant human ROCK2 protein is a Human Fragment protein, in the 5 to 554 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, WB, FuncS.
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Key facts
- Purity
- >95% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.
Alternative names
KIAA0619, ROCK2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, ROCK-II
Recommended products
Recombinant human ROCK2 protein is a Human Fragment protein, in the 5 to 554 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >95% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab125828 was determined to be 605 nmol/min/mg.
- Accession
- O75116-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.71% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence
- Accession
- O75116
- Protein length
- Fragment
- Predicted molecular weight
- 66 kDa
- Amino acids
- 5 to 554
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purity was determined to be >95% by densitometry. Affinity purified.
General info
- Function
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.
- Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
- Post-translational modifications
Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity.
- Subcellular localisation
- Nucleus, Cytoskeleton, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
RPS6 peptide ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
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2 product images
SDS-PAGE - Recombinant human ROCK2 protein (ab125828)
SDS-PAGE analysis of ab125828.
Functional Studies - Recombinant human ROCK2 protein (ab125828)
The specific activity of ab125828 was determined to be 605 nmol/min/mg.
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