Recombinant Human RON protein (His tag) is a Human Fragment protein, in the 1 to 571 aa range, expressed in HEK 293, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M E L L P P L P Q S F L L L L L L P A K P A A G E D W Q C P R T P Y A A S R D F D V K Y V V P S F S A G G L V Q A M V T Y E G D R N E S A V F V A I R N R L H V L G P D L K S V Q S L A T G P A G D P G C Q T C A A C G P G P H G P P G D T D T K V L V L D P A L P A L V S C G S S L Q G R C F L H D L E P Q G T A V H L A A P A C L F S A H H N R P D D C P D C V A S P L G T R V T V V E Q G Q A S Y F Y V A S S L D A A V A A S F S P R S V S I R R L K A D A S G F A P G F V A L S V L P K H L V S Y S I E Y V H S F H T G A F V Y F L T V Q P A S V T D D P S A L H T R L A R L S A T E P E L G D Y R E L V L D C R F A P K R R R R G A P E G G Q P Y P V L R V A H S A P V G A Q L A T E L S I A E G Q E V L F G V F V T G K D G G P G V G P N S V V C A F P I D L L D T L I D E G V E R C C E S P V H P G L R R G L D F F Q S P S F C P N P P G L E A L S P N T S C R H F P L L V S S S F S R V D L F N G L L G P V Q V T A L Y V T R L D N V T V A H M G T M D G R I L Q V E L V R S L N Y L L Y V S N F S L G D S G Q P V Q R D V S R L G D H L L F A S G D Q V F Q V P I Q G P G C R H F L T C G R C L R A W H F M G C G W C G N M C G Q Q K E C P G S W Q Q D H C P P K L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Also plays a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.
Alternative names
CD136, PTK8, RON, MST1R, Macrophage-stimulating protein receptor, MSP receptor, CDw136, Protein-tyrosine kinase 8, p185-Ron
Recommended products
Recombinant Human RON protein (His tag) is a Human Fragment protein, in the 1 to 571 aa range, expressed in HEK 293, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q04912-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M E L L P P L P Q S F L L L L L L P A K P A A G E D W Q C P R T P Y A A S R D F D V K Y V V P S F S A G G L V Q A M V T Y E G D R N E S A V F V A I R N R L H V L G P D L K S V Q S L A T G P A G D P G C Q T C A A C G P G P H G P P G D T D T K V L V L D P A L P A L V S C G S S L Q G R C F L H D L E P Q G T A V H L A A P A C L F S A H H N R P D D C P D C V A S P L G T R V T V V E Q G Q A S Y F Y V A S S L D A A V A A S F S P R S V S I R R L K A D A S G F A P G F V A L S V L P K H L V S Y S I E Y V H S F H T G A F V Y F L T V Q P A S V T D D P S A L H T R L A R L S A T E P E L G D Y R E L V L D C R F A P K R R R R G A P E G G Q P Y P V L R V A H S A P V G A Q L A T E L S I A E G Q E V L F G V F V T G K D G G P G V G P N S V V C A F P I D L L D T L I D E G V E R C C E S P V H P G L R R G L D F F Q S P S F C P N P P G L E A L S P N T S C R H F P L L V S S S F S R V D L F N G L L G P V Q V T A L Y V T R L D N V T V A H M G T M D G R I L Q V E L V R S L N Y L L Y V S N F S L G D S G Q P V Q R D V S R L G D H L L F A S G D Q V F Q V P I Q G P G C R H F L T C G R C L R A W H F M G C G W C G N M C G Q Q K E C P G S W Q Q D H C P P K L
- Accession
- Q04912
- Protein length
- Fragment
- Predicted molecular weight
- 60 kDa
- Amino acids
- 1 to 571
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Also plays a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family.
- Post-translational modifications
Proteolytic processing yields the two subunits.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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SDS-PAGE - Recombinant Human RON protein (His tag) (ab276409)
SDS-PAGE analysis of ab276409
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