Recombinant Human RPB3 protein is a Human Full Length protein, in the 1 to 275 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M P Y A N Q P T V R I T E L T D E N V K F I I E N T D L A V A N S I R R V F I A E V P I I A I D W V Q I D A N S S V L H D E F I A H R L G L I P L I S D D I V D K L Q Y S R D C T C E E F C P E C S V E F T L D V R C E D Q T R H V T S R D L I S N S R V I P V T S R N R D N D P N D Y V E Q D D I L I V K L R K G Q E L R L R A Y A K K G F G K E H A K W N P T A G V A F E Y D P D N A L R H T V Y P K P E E W P K S E Y S E L D E D E S Q A P Y D P N G K P E R F Y Y N V E S C G S L R P E T I V L S A L S G L K K K L S D L Q T Q L S H E I Q S D V L T I N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates.
Alternative names
A-152E5.7, POLR2C, DNA-directed RNA polymerase II subunit RPB3, RNA polymerase II subunit 3, RNA polymerase II subunit B3, DNA-directed RNA polymerase II 33 kDa polypeptide, DNA-directed RNA polymerase II subunit C, RPB31, RPB33
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Recombinant Human RPB3 protein is a Human Full Length protein, in the 1 to 275 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P19387-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M P Y A N Q P T V R I T E L T D E N V K F I I E N T D L A V A N S I R R V F I A E V P I I A I D W V Q I D A N S S V L H D E F I A H R L G L I P L I S D D I V D K L Q Y S R D C T C E E F C P E C S V E F T L D V R C E D Q T R H V T S R D L I S N S R V I P V T S R N R D N D P N D Y V E Q D D I L I V K L R K G Q E L R L R A Y A K K G F G K E H A K W N P T A G V A F E Y D P D N A L R H T V Y P K P E E W P K S E Y S E L D E D E S Q A P Y D P N G K P E R F Y Y N V E S C G S L R P E T I V L S A L S G L K K K L S D L Q T Q L S H E I Q S D V L T I N
- Accession
- P19387
- Protein length
- Full Length
- Predicted molecular weight
- 33.8 kDa
- Amino acids
- 1 to 275
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab181910 was purified using conventional chromatography techniques.
General info
- Function
Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates.
- Sequence similarities
Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Previously labelled as POLR2C.
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1 product image
SDS-PAGE - Recombinant Human RPB3 protein (ab181910)
15% SDS-PAGE analysis of ab181910 (3μg).
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