Recombinant Human RPC39 protein is a Human Full Length protein, in the 1 to 316 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A E V K V K V Q P P D A D P V E I E N R I I E L C H Q F P H G I T D Q V I Q N E M P H I E A Q Q R A V A I N R L L S M G Q L D L L R S N T G L L Y R I K D S Q N A G K M K G S D N Q E K L V Y Q I I E D A G N K G I W S R D I R Y K S N L P L T E I N K I L K N L E S K K L I K A V K S V A A S K K K V Y M L Y N L Q P D R S V T G G A W Y S D Q D F E S E F V E V L N Q Q C F K F L Q S K A E T A R E S K Q N P M I Q R N S S F A S S H E V W K Y I C E L G I S K V E L S M E D I E T I L N T L I Y D G K V E M T I I A A K E G T V G S V D G H M K L Y R A V N P I I P P T G L V R A P C G L C P V F D D C H E G G E I S P S N C I Y M T E W L E F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed:20413673, PubMed:21358628, PubMed:33558764, PubMed:34675218). Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Part of POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer that coordinates the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state (PubMed:20413673, PubMed:33558764, PubMed:33558766). Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses, including varicella zoster virus. Acts as a nuclear and cytosolic DNA sensor detecting AT-rich DNA, involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway (PubMed:19609254, PubMed:19631370, PubMed:30211253). Preferentially binds double-stranded DNA (dsDNA) (PubMed:21358628).
Alternative names
DNA-directed RNA polymerase III subunit RPC6, RNA polymerase III subunit C6, DNA-directed RNA polymerase III subunit F, RNA polymerase III 39 kDa subunit, RPC39, POLR3F
Recommended products
Recombinant Human RPC39 protein is a Human Full Length protein, in the 1 to 316 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9H1D9-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M A E V K V K V Q P P D A D P V E I E N R I I E L C H Q F P H G I T D Q V I Q N E M P H I E A Q Q R A V A I N R L L S M G Q L D L L R S N T G L L Y R I K D S Q N A G K M K G S D N Q E K L V Y Q I I E D A G N K G I W S R D I R Y K S N L P L T E I N K I L K N L E S K K L I K A V K S V A A S K K K V Y M L Y N L Q P D R S V T G G A W Y S D Q D F E S E F V E V L N Q Q C F K F L Q S K A E T A R E S K Q N P M I Q R N S S F A S S H E V W K Y I C E L G I S K V E L S M E D I E T I L N T L I Y D G K V E M T I I A A K E G T V G S V D G H M K L Y R A V N P I I P P T G L V R A P C G L C P V F D D C H E G G E I S P S N C I Y M T E W L E F
- Accession
- Q9H1D9
- Protein length
- Full Length
- Predicted molecular weight
- 38.1 kDa
- Amino acids
- 1 to 316
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab139235 is purified using conventional chromatography techniques.
General info
- Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed:20413673, PubMed:21358628, PubMed:33558764, PubMed:34675218). Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Part of POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer that coordinates the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state (PubMed:20413673, PubMed:33558764, PubMed:33558766). Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses, including varicella zoster virus. Acts as a nuclear and cytosolic DNA sensor detecting AT-rich DNA, involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway (PubMed:19609254, PubMed:19631370, PubMed:30211253). Preferentially binds double-stranded DNA (dsDNA) (PubMed:21358628).
- Sequence similarities
Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Previously labelled as POLR3F.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human RPC39 protein (ab139235)
15% SDS-PAGE analysis of 3 µg ab139235.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com