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AB139235

Recombinant Human RPC39 protein

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Recombinant Human RPC39 protein is a Human Full Length protein, in the 1 to 316 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

DNA-directed RNA polymerase III subunit RPC6, RNA polymerase III subunit C6, DNA-directed RNA polymerase III subunit F, RNA polymerase III 39 kDa subunit, RPC39, POLR3F

1 Images
SDS-PAGE - Recombinant Human RPC39 protein (AB139235)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RPC39 protein (AB139235)

15% SDS-PAGE analysis of 3 µg ab139235.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9H1D9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as POLR3F.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAEVKVKVQPPDADPVEIENRIIELCHQFPHGITDQVIQNEMPHIEAQQRAVAINRLLSMGQLDLLRSNTGLLYRIKDSQNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYMLYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARESKQNPMIQRNSSFASSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYRAVNPIIPPTGLVRAPCGLCPVFDDCHEGGEISPSNCIYMTEWLEF","proteinLength":"Full Length","predictedMolecularWeight":"38.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":316,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9H1D9","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab139235 is purified using conventional chromatography techniques.

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General info

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed : 20413673, PubMed : 21358628, PubMed : 33558764, PubMed : 34675218). Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Part of POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer that coordinates the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state (PubMed : 20413673, PubMed : 33558764, PubMed : 33558766). Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses, including varicella zoster virus. Acts as a nuclear and cytosolic DNA sensor detecting AT-rich DNA, involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway (PubMed : 19609254, PubMed : 19631370, PubMed : 30211253). Preferentially binds double-stranded DNA (dsDNA) (PubMed : 21358628).

Sequence similarities

Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed : 20413673, PubMed : 21358628, PubMed : 33558764, PubMed : 34675218). Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Part of POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer that coordinates the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state (PubMed : 20413673, PubMed : 33558764, PubMed : 33558766). Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses, including varicella zoster virus. Acts as a nuclear and cytosolic DNA sensor detecting AT-rich DNA, involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway (PubMed : 19609254, PubMed : 19631370, PubMed : 30211253). Preferentially binds double-stranded DNA (dsDNA) (PubMed : 21358628).
See full target information POLR3F
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Complementary Products

Primary Antibodies

AB250204

Anti-RPC39 antibody [EPR13200] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-RPC39 antibody [EPR13200] - BSA and Azide free (AB250204)

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