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AB140558

Recombinant Human RPL12 protein

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Recombinant Human RPL12 protein is a Human Full Length protein, in the 1 to 165 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Large ribosomal subunit protein uL11, 60S ribosomal protein L12, RPL12

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SDS-PAGE - Recombinant Human RPL12 protein (AB140558)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human RPL12 protein (AB140558)

15% SDS-PAGE analysis of ab140558 (3μg)

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P30050

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMPPKFDPNEIKVVYLRCTGGEVGATSALAPKIGPLGLSPKKVGDDIAKATGDWKGLRITVKLTIQNRQAQIEVVPSASALIIKALKEPPRDRKKQKNIKHSGNITFDEIVNIARQMRHRSLARELSGTIKEILGTAQSVGCNVDGRHPHDIIDDINSGAVECPAS","proteinLength":"Full Length","predictedMolecularWeight":"20.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":165,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P30050","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab140558 was purified using conventional chromatography.

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General info

Function

Component of the large ribosomal subunit (PubMed : 25901680). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 25901680). Binds directly to 26S ribosomal RNA (PubMed : 25901680).

Sequence similarities

Belongs to the universal ribosomal protein uL11 family.

Post-translational modifications

Ubiquitinated at Lys-48 and Lys-83 by RNF14 and RNF25 in response to ribosome collisions (ribosome stalling).

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Product protocols

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Target data

Component of the large ribosomal subunit (PubMed : 25901680). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 25901680). Binds directly to 26S ribosomal RNA (PubMed : 25901680).
See full target information 60S ribosomal protein L12
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Complementary Products

Primary Antibodies

AB127533

Anti-RPL12 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL12 antibody (AB127533)

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