Recombinant Human RPL18A protein (denatured) is a Human Full Length protein, in the 1 to 176 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M K A S G T L R E Y K V V G R C L P T P K C H T P P L Y R M R I F A P N H V V A K S R F W Y F V S Q L K K M K K S S G E I V Y C G Q V F E K S P L R V K N F G I W L R Y D S R S G T H N M Y R E Y R D L T T A G A V T Q C Y R D M G A R H R A R A H S I Q I M K V E E I A A S K C R R P A V K Q F H D S K I K F P L P H R V L R R Q H K P R F T T K R P N T F F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Alternative names
Large ribosomal subunit protein eL20, 60S ribosomal protein L18a, RPL18A
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Recombinant Human RPL18A protein (denatured) is a Human Full Length protein, in the 1 to 176 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q02543-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M K A S G T L R E Y K V V G R C L P T P K C H T P P L Y R M R I F A P N H V V A K S R F W Y F V S Q L K K M K K S S G E I V Y C G Q V F E K S P L R V K N F G I W L R Y D S R S G T H N M Y R E Y R D L T T A G A V T Q C Y R D M G A R H R A R A H S I Q I M K V E E I A A S K C R R P A V K Q F H D S K I K F P L P H R V L R R Q H K P R F T T K R P N T F F
- Accession
- Q02543
- Protein length
- Full Length
- Predicted molecular weight
- 23.2 kDa
- Amino acids
- 1 to 176
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
- Sequence similarities
Belongs to the eukaryotic ribosomal protein eL20 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RPL18A also known as 60S ribosomal protein L18a plays a part in ribosome structure and function. This ribosomal protein with a molecular mass of approximately 22 kDa is an important part of the large 60S subunit of the ribosome. It is present in various tissues and has ubiquitous expression reflecting its role in protein synthesis. It functions as a structural constituent and it interacts directly with ribosomal RNA to help create the core framework necessary for ribosome stability.
- Biological function summary
Ribosomal protein L18a contributes to the assembly and function of ribosomes the essential machines for translation of mRNA into proteins. It is a part of the ribosomal complex which includes numerous proteins and RNA molecules that collaborate for translation. L18a assists in maintaining the proper structure needed for the translation process impacting cell growth and proliferation. Also ribosomal proteins like RPL18A have non-ribosomal roles in regulating cell cycle and apoptosis showing their importance beyond protein synthesis.
- Pathways
Ribosome biogenesis and protein synthesis involve RPL18A. It is a component of the ribosomal pathway closely related to other ribosomal proteins like RPL5 and RPL11 essential for the synthesis of new proteins. RPL18A also intersects with the mTOR signaling pathway which senses nutritional and energy conditions to regulate cell growth emphasizing its role in metabolic control.
- Associated diseases and disorders
Mutations or dysregulation of RPL18A connect to conditions such as Diamond-Blackfan anemia a rare genetic disorder resulting in failure to make red blood cells. RPL18A mutation leads to disrupted ribosome biogenesis similarly observed in many cancers where rapid cell growth demands increased protein synthesis. The interaction between RPL18A and other ribosomal proteins like RPL11 suggests their combined influence on the progression of these disorders by altering ribosomal function and cellular homeostasis.
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1 product image
SDS-PAGE - Recombinant Human RPL18A protein (denatured) (ab180319)
15% SDS-PAGE analysis of ab180319 (3μg)
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