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AB156337

Recombinant Human RPL23A protein

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Recombinant Human RPL23A protein is a Human Full Length protein, in the 1 to 156 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Large ribosomal subunit protein uL23, 60S ribosomal protein L23a, RPL23A

1 Images
SDS-PAGE - Recombinant Human RPL23A protein (AB156337)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RPL23A protein (AB156337)

15% SDS-PAGE analysis of ab156337 (3µg).

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P62750

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Preservative: 1.7% Imidazole Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAPKAKKEAPAPPKAEAKAKALKAKKAVLKGVHSHKKKKIRTSPTFRRPKTLRLRRQPKYPRKSAPRRNKLDHYAIIKFPLTTESAMKKIEDNNTLVFIVDVKANKHQIKQAVKKLYDIDVAKVNTLIRPDGEKKAYVRLAPDYDALDVANKIGII","proteinLength":"Full Length","predictedMolecularWeight":"20.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":156,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P62750","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL23A also known as Ribosomal Protein L23a is a component of the 60S subunit in the ribosome which is responsible for protein synthesis. It has a molecular mass of approximately 15 kDa. RPL23A is expressed in various tissues but shows significant expression levels in the liver and pancreas. The protein plays a mechanical role by ensuring structural stability and proper functioning of the ribosome during translation.
Biological function summary

RPL23A participates in the assembly and function of the ribosomal complex critical for translation. Within this complex RPL23A contributes to the coordination of protein synthesis by helping to align mRNA and tRNA correctly. This alignment is essential for the catalysis of peptide bond formation between amino acids allowing for the accurate synthesis of proteins.

Pathways

RPL23A is integral to the mTOR signaling pathway and the p53 signaling pathway. The mTOR pathway regulates cell growth proliferation and survival in response to nutrients growth factors and cellular energy levels with RPL23A interacting with proteins like TSC1 and TSC2. In the p53 signaling pathway RPL23A can influence cellular responses to DNA damage and stress by interacting with proteins such as MDM2.

RPL23A is implicated in cancer and Diamond-Blackfan anemia. In cancer the dysregulation of RPL23A can affect ribosome biogenesis and cell proliferation often seen in conjunction with increased levels of proteins like c-Myc. In Diamond-Blackfan anemia mutations or malfunctions in RPL23A lead to defective ribosomal function impacting erythroid progenitor cells and causing anemia with links to other ribosomal proteins such as RPS19.
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Specifications

Form

Liquid

Additional notes

ab156337 is purified using conventional chromatography techniques.

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General info

Function

Component of the large ribosomal subunit (PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 32669547). Binds a specific region on the 26S rRNA (PubMed : 23636399, PubMed : 32669547). May promote p53/TP53 degradation possibly through the stimulation of MDM2-mediated TP53 polyubiquitination (PubMed : 26203195).

Sequence similarities

Belongs to the universal ribosomal protein uL23 family.

Post-translational modifications

N-terminus is methylated by METTL11A/NTM1.. Citrullinated by PADI4.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the large ribosomal subunit (PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 32669547). Binds a specific region on the 26S rRNA (PubMed : 23636399, PubMed : 32669547). May promote p53/TP53 degradation possibly through the stimulation of MDM2-mediated TP53 polyubiquitination (PubMed : 26203195).
See full target information 60S ribosomal protein L23a
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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