Recombinant Human RPL34 protein (denatured) is a Human Full Length protein, in the 1 to 117 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M V Q R L T Y R R R L S Y N T A S N K T R L S R T P G N R I V Y L Y T K K V G K A P K S A C G V C P G R L R G V R A V R P K V L M R L S K T K K H V S R A Y G G S M C A K C V R D R I K R A F L I E E Q K I V V K V L K A Q A Q S Q K A K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the large ribosomal subunit (PubMed:12962325, PubMed:23636399, PubMed:25901680, PubMed:25957688, PubMed:32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:12962325, PubMed:23636399, PubMed:25901680, PubMed:25957688, PubMed:32669547).
Alternative names
Large ribosomal subunit protein eL34, 60S ribosomal protein L34, RPL34
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Recombinant Human RPL34 protein (denatured) is a Human Full Length protein, in the 1 to 117 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P49207-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.34% Sodium chloride, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M V Q R L T Y R R R L S Y N T A S N K T R L S R T P G N R I V Y L Y T K K V G K A P K S A C G V C P G R L R G V R A V R P K V L M R L S K T K K H V S R A Y G G S M C A K C V R D R I K R A F L I E E Q K I V V K V L K A Q A Q S Q K A K
- Accession
- P49207
- Protein length
- Full Length
- Predicted molecular weight
- 15.7 kDa
- Amino acids
- 1 to 117
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Component of the large ribosomal subunit (PubMed:12962325, PubMed:23636399, PubMed:25901680, PubMed:25957688, PubMed:32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:12962325, PubMed:23636399, PubMed:25901680, PubMed:25957688, PubMed:32669547).
- Sequence similarities
Belongs to the eukaryotic ribosomal protein eL34 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The RPL34 protein also known as ribosomal protein L34 is integral to the function of ribosomes where it plays a role in protein synthesis. It is a component of the 60S large ribosomal subunit and has a molecular mass of approximately 13 kDa. RPL34 is expressed in a wide range of tissues reflecting its essential role in the general process of translation. The conservation of RPL34 across species highlights its fundamental function in cellular machinery.
- Biological function summary
RPL34 participates in the assembly and function of the ribosomal complex essential for translating mRNA into proteins. As part of the ribosome it interacts with rRNA and other ribosomal proteins to form a functional unit necessary for protein synthesis. The activity of RPL34 ensures accurate translation and effective cellular protein production reinforcing the ribosome's role as the cell's protein factory. Disruptions in its function can lead to impaired cellular processes due to defective protein production.
- Pathways
RPL34 plays a vital role in the translation pathway where it ensures the correct assembly and function of the ribosomal complex. It is part of the ribosome biogenesis pathway which involves numerous other ribosomal proteins and factors. Its interaction with other ribosomal proteins such as RPL5 and RPL11 ensures efficient ribosomal assembly and function which is important for cellular homeostasis. The proper function of these pathways is important for maintaining cellular growth and division.
- Associated diseases and disorders
Mutations or dysregulation in RPL34 can be linked to ribosomopathies which include Diamond-Blackfan anemia a disorder characterized by anemia and congenital abnormalities. Research also suggests a connection between RPL34 and cancer where altered expression levels can contribute to tumorigenesis via the deregulation of ribosome biogenesis. The link with proteins like MDM2 involved in ribosome activity and cell cycle regulation further implicates RPL34 in pathways that could lead to disease when aberrantly expressed or mutated.
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1 product image
SDS-PAGE - Recombinant Human RPL34 protein (denatured) (ab171711)
15% SDS-PAGE analysis of ab171711 (3μg).
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