JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB187442

Recombinant Human RPL35 protein (denatured) (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human RPL35 protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 123 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE.

View Alternative Names

Large ribosomal subunit protein uL29, 60S ribosomal protein L35, RPL35

1 Images
SDS-PAGE - Recombinant Human RPL35 protein (denatured) (His tag N-Terminus) (AB187442)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human RPL35 protein (denatured) (His tag N-Terminus) (AB187442)

15% SDS-PAGE analysis of ab187442 (3μg).

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P42766

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAKIKARDLRGKKKEELLKQLDDLKVELSQLRVAKVTGGAASKLSKIRVVRKSIARVLTVINQTQKENLRKFYKGKKYKPLDLRPKKTRAMRRRLNKHEENLKTKKQQRKERLYPLRKYAVKA","proteinLength":"Full Length","predictedMolecularWeight":"16.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":123,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P42766","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL35 also known as Ribosomal Protein L35 plays an essential role in ribosome function. It is a component of the large 60S ribosomal subunit and has a molecular mass of around 13.4 kDa. RPL35 exhibits widespread expression in human tissues suggesting it is necessary for general protein synthesis functions across different cell types.
Biological function summary

The function of RPL35 contributes to the formation and stability of the ribosome which is a complex structure composed of ribosomal RNA and ribosomal proteins. RPL35's integration into the ribosome assists in the assembly of new proteins by ensuring correct translation. This integration is necessary for maintaining the efficiency and accuracy of protein synthesis within cells playing an important role in cell growth and proliferation.

Pathways

The activity of RPL35 influences the mRNA translation pathway and connects closely to the protein biosynthesis process. Through its interaction with the ribosomal machinery it also relates to the mTOR signaling pathway which is significant for cell growth regulation. Proteins like RPLP0 and RPLP1 are associated with RPL35 within these pathways supporting a robust translation and protein synthesis network.

Mutations and dysregulation of RPL35 relate to disorders like Diamond-Blackfan anemia and some cancers. These conditions link to the disruption in ribosome biogenesis or function which leads to faulty protein synthesis. In Diamond-Blackfan anemia abnormal function of RPL35 along with other ribosomal proteins such as RPL5 disrupts normal erythropoiesis resulting in anemia.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547).

Sequence similarities

Belongs to the universal ribosomal protein uL29 family.

style
left-column

Product protocols

style
left-column

Target data

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547).
See full target information 60S ribosomal protein L35
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB187441

Recombinant Human GTF3C6 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human GTF3C6 protein (His tag N-Terminus) (AB187441)

  • 0
  • 0
Primary Antibodies

AB190162

Anti-RPL35 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (AB190162)

  • 0
  • 0
Primary Antibodies

AB302891

Anti-PKC theta/PRKCQ antibody [27/PKC theta]

Recombinant|Trial Size

Immunoprecipitation - Anti-PKC theta/PRKCQ antibody [27/PKC theta] (AB302891)

  • 0
  • 0
Proteins & Peptides

AB204141

Recombinant human NEK8 protein

Functional Studies - Recombinant human NEK8 protein (AB204141)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com