Recombinant Human RPL5 protein (His tag N-Terminus)
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(1 Publication)
Recombinant Human RPL5 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 297 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
MSTP030, RPL5, Large ribosomal subunit protein uL18, 60S ribosomal protein L5
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human RPL5 protein (His tag N-Terminus) (AB183222)
15% SDS-PAGE analysis of ab183222 (3μg).
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The actions of RPL5 include its involvement in the incorporation of the 5S rRNA into the ribosomal subunit in cooperation with other ribosomal proteins. RPL5 is part of the ribosomal complex which is fundamental for translating mRNA into polypeptides. By binding with 5S rRNA RPL5 ensures the structural integrity and functional competence of the ribosome which are important for gene expression and cellular homeostasis.
Pathways
RPL5 plays a critical role in the ribosome biogenesis pathway and indirectly influences the p53 pathway. It interacts with RPL11 to regulate the p53 response during ribosomal stress which connects RPL5 to the ribosomal stress checkpoint pathway. Deviations in ribosomal assembly or function can influence processes linked to cell cycle regulation and apoptosis.
Specifications
Form
Liquid
Additional notes
ab183222 was purified using conventional chromatography techniques.
General info
Function
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs (PubMed : 12962325, PubMed : 19061985, PubMed : 23636399, PubMed : 24120868). It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53 (PubMed : 24120868).
Sequence similarities
Belongs to the universal ribosomal protein uL18 family.
Subcellular localisation
Nucleus
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Science advances 7:eabi5751 PubMed34890234
2021
Applications
Unspecified application
Species
Unspecified reactive species
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