Recombinant Human RPL7A protein (denatured) is a Human Full Length protein, in the 1 to 266 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M P K G K K A K G K K V A P A P A V V K K Q E A K K V V N P L F E K R P K N F G I G Q D I Q P K R D L T R F V K W P R Y I R L Q R Q R A I L Y K R L K V P P A I N Q F T Q A L D R Q T A T Q L L K L A H K Y R P E T K Q E K K Q R L L A R A E K K A A G K G D V P T K R P P V L R A G V N T V T T L V E N K K A Q L V V I A H D V D P I E L V V F L P A L C R K M G V P Y C I I K G K A R L G R L V H R K T C T T V A F T Q V N S E D K G A L A K L V E A I R T N Y N D R Y D E I R R H W G G N V L G P K S V A R I A K L E K A K A K E L A T K L G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the large ribosomal subunit (PubMed:23636399, PubMed:32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399, PubMed:32669547).
Alternative names
SURF-3, SURF3, RPL7A, Large ribosomal subunit protein eL8, 60S ribosomal protein L7a, PLA-X polypeptide, Surfeit locus protein 3
Recommended products
Recombinant Human RPL7A protein (denatured) is a Human Full Length protein, in the 1 to 266 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P62424-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M P K G K K A K G K K V A P A P A V V K K Q E A K K V V N P L F E K R P K N F G I G Q D I Q P K R D L T R F V K W P R Y I R L Q R Q R A I L Y K R L K V P P A I N Q F T Q A L D R Q T A T Q L L K L A H K Y R P E T K Q E K K Q R L L A R A E K K A A G K G D V P T K R P P V L R A G V N T V T T L V E N K K A Q L V V I A H D V D P I E L V V F L P A L C R K M G V P Y C I I K G K A R L G R L V H R K T C T T V A F T Q V N S E D K G A L A K L V E A I R T N Y N D R Y D E I R R H W G G N V L G P K S V A R I A K L E K A K A K E L A T K L G
- Accession
- P62424
- Protein length
- Full Length
- Predicted molecular weight
- 32.4 kDa
- Amino acids
- 1 to 266
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Component of the large ribosomal subunit (PubMed:23636399, PubMed:32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399, PubMed:32669547).
- Sequence similarities
Belongs to the eukaryotic ribosomal protein eL8 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The RPL7A protein also known as 60S ribosomal protein L7a plays a role in the structure and function of the ribosome. It is a component of the large 60S subunit of the ribosome and has a mass of approximately 29.5 kDa. RPL7A is expressed in various tissues and is involved in protein synthesis by forming part of the ribosome machinery that translates mRNA into protein.
- Biological function summary
RPL7A is part of the ribosomal complex which is essential for the translation process in cells. This protein interacts with rRNA and other ribosomal proteins to ensure the proper assembly and function of ribosomes. Its function is necessary for maintaining the fidelity and efficiency of protein synthesis. RPL7A’s presence in the ribosome allows it to influence cellular growth and proliferation by affecting how effectively proteins are produced within the cell.
- Pathways
RPL7A plays a role in the translation pathway which is critical for protein biosynthesis. This pathway involves other proteins such as RPL5 and RPL11 which also participate in ribosomal subunit formation and function. Additionally RPL7A is part of the mTOR signaling pathway a major regulator of cellular growth in which it helps modulate ribosome biogenesis and protein synthesis in response to nutrient availability.
- Associated diseases and disorders
Mutations or dysregulation in RPL7A have been associated with certain cancers. For instance alterations in RPL7A expression levels can disrupt normal ribosome function contributing to the pathogenesis of leukemia. The interaction of RPL7A with proteins like p53 links it to cell cycle regulation where it can influence tumor development and progression when these interactions are impaired.
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1 product image
SDS-PAGE - Recombinant Human RPL7A protein (denatured) (ab187474)
ab187474 (3 μg) on 15 % SDS PAGE
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