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AB123188

Recombinant Human RPL8 protein (His tag N-Terminus)

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(1 Publication)

Recombinant Human RPL8 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 257 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Large ribosomal subunit protein uL2, 60S ribosomal protein L8, RPL8

1 Images
SDS-PAGE - Recombinant Human RPL8 protein (His tag N-Terminus) (AB123188)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human RPL8 protein (His tag N-Terminus) (AB123188)

3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P62917

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGRVIRGQRKGAGSVFRAHVKHRKGAARLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAKVVFRDPYRFKKRTELFIAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQEKEN","proteinLength":"Full Length","predictedMolecularWeight":"30.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":257,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P62917","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPL8 also known as Ribosomal Protein L8 is a component of the 60S subunit of the ribosome playing a critical role in protein synthesis. It has a mass of approximately 26.1 kDa. RPL8 is expressed in a wide range of tissues including the liver kidney and brain reflecting its essential role in cellular function. This protein contributes directly to the structure and function of the ribosome facilitating the translation of mRNA into polypeptides.
Biological function summary

RPL8 serves as an integral part of the ribosomal machinery ensuring effective protein synthesis within cells. It is part of the large ribosomal subunit complex working alongside other ribosomal proteins to form a functional ribosome. This role is paramount for maintaining cellular homeostasis and supporting growth and proliferation by enabling balanced protein production.

Pathways

RPL8 is key within several fundamental biological pathways including the translation pathway and ribosome biogenesis. In ribosome biogenesis it interacts with proteins such as RPL5 and RPL11 contributing to the assembly and maturation of ribosomal subunits. This interaction facilitates the complex process of protein synthesis ensuring the correct translation of genetic information into functional proteins.

Abnormalities in RPL8 expression or function have been linked to certain cancers and Diamond-Blackfan anemia. In cancer dysfunction in ribosomal protein balance including RPL8 can lead to unchecked cellular proliferation. In Diamond-Blackfan anemia defects associated with RPL8 and other ribosomal proteins like RPS19 cause ineffective erythropoiesis leading to the anemia typically observed in this condition. Understanding these connections helps guide potential therapeutic interventions.
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Specifications

Form

Liquid

Additional notes

ab123188 is purified using conventional chromatography techniques after refolding of the isolated inclusion bodies in a renaturation buffer.

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General info

Function

Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

Sequence similarities

Belongs to the universal ribosomal protein uL2 family.

Post-translational modifications

Hydroxylated on His-216 by RIOX1. The modification is impaired by hypoxia.

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Product protocols

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Target data

Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
See full target information RPL8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 7:eabi5751 PubMed34890234

2021

The nuclear transcription factor, TAF7, is a cytoplasmic regulator of protein synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Cheng,Kevin Semmens,Elizabeth McManus,Qingrong Chen,Daoud Meerzaman,Xiantao Wang,Markus Hafner,Brian A Lewis,Hidehisa Takahashi,Ballachanda N Devaiah,Anne Gegonne,Dinah S Singer
View all publications
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