Recombinant Human RPS2 protein (denatured) is a Human Full Length protein, in the 1 to 293 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A D D A G A A G G P G G P G G P G M G N R G G F R G G F G S G I R G R G R G R G R G R G R G R G A R G G K A E D K E W M P V T K L G R L V K D M K I K S L E E I Y L F S L P I K E S E I I D F F L G A S L K D E V L K I M P V Q K Q T R A G Q R T R F K A F V A I G D Y N G H V G L G V K C S K E V A T A I R G A I I L A K L S I V P V R R G Y W G N K I G K P H T V P C K V T G R C G S V L V R L I P A P R G T G I V S A P V P K K L L M M A G I D D C Y T S A R G C T A T L G N F A K A T F D A I S K T Y S Y L T P D L W K E T V F T K S P Y Q E F T D H L V K T H T R V S V Q R T Q A P A V A T T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399). The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules (PubMed:23636399). The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain (PubMed:23636399). The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (PubMed:23636399). Plays a role in the assembly and function of the 40S ribosomal subunit (By similarity). Mutations in this protein affects the control of translational fidelity (By similarity). Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly (By similarity).
Alternative names
RPS4, RPS2, Small ribosomal subunit protein uS5, 40S ribosomal protein S2, 40S ribosomal protein S4, Protein LLRep3
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Recombinant Human RPS2 protein (denatured) is a Human Full Length protein, in the 1 to 293 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P15880-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M A D D A G A A G G P G G P G G P G M G N R G G F R G G F G S G I R G R G R G R G R G R G R G R G A R G G K A E D K E W M P V T K L G R L V K D M K I K S L E E I Y L F S L P I K E S E I I D F F L G A S L K D E V L K I M P V Q K Q T R A G Q R T R F K A F V A I G D Y N G H V G L G V K C S K E V A T A I R G A I I L A K L S I V P V R R G Y W G N K I G K P H T V P C K V T G R C G S V L V R L I P A P R G T G I V S A P V P K K L L M M A G I D D C Y T S A R G C T A T L G N F A K A T F D A I S K T Y S Y L T P D L W K E T V F T K S P Y Q E F T D H L V K T H T R V S V Q R T Q A P A V A T T
- Accession
- P15880
- Protein length
- Full Length
- Predicted molecular weight
- 33.7 kDa
- Amino acids
- 1 to 293
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399). The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules (PubMed:23636399). The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain (PubMed:23636399). The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (PubMed:23636399). Plays a role in the assembly and function of the 40S ribosomal subunit (By similarity). Mutations in this protein affects the control of translational fidelity (By similarity). Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly (By similarity).
- Sequence similarities
Belongs to the universal ribosomal protein uS5 family.
- Post-translational modifications
Citrullinated by PADI4 in the Arg/Gly-rich region.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RPS2 also known as Ribosomal Protein S2 is a component of the 40S subunit of the ribosome. This protein plays a mechanical role in ribosome assembly and protein synthesis. RPS2 has a molecular mass of approximately 29 kDa. Researchers have observed its expression in various tissues indicating its involvement in general cellular processes. It facilitates the initiation of translation by binding to ribosomal RNA and other ribosomal proteins to form a functional ribosome.
- Biological function summary
Ribosomal Protein S2 contributes significantly to ribosome function and protein translation. As part of the ribosome RPS2 aids in the decoding of messenger RNA (mRNA) into proteins which occurs in all cellular environments. This protein works in conjunction with other ribosomal proteins and rRNA to ensure accuracy and efficiency in protein synthesis. It forms an integral part of the ribosomal machinery that also includes many other proteins and RNA molecules congregating to form the translation initiation complex.
- Pathways
Several biological systems depend on the role that Ribosomal Protein S2 plays in protein synthesis. The mTOR signaling pathway is particularly important as RPS2 and other ribosomal proteins function under the regulation of mTOR to control protein translation rates. Additionally RPS2 connects with proteins like eIF2 to influence translation initiation. Together they regulate critical processes such as cell growth and proliferation linking ribosomal function with cellular metabolic status.
- Associated diseases and disorders
Impaired Ribosomal Protein S2 function can lead to conditions such as Diamond-Blackfan anemia which results from defects in ribosome biogenesis. Mutations or disruptions in related proteins like RPS19 are often studied alongside RPS2 for their roles in hereditary and acquired anemias. Furthermore aberrant RPS2 expression can contribute to tumorigenesis implicating it in cancer studies where proteins like p53 may interact or affect its function revealing the broader implications of ribosomal proteins in human health and diseases.
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1 product image
SDS-PAGE - Recombinant Human RPS2 protein (denatured) (ab171474)
15% SDS-PAGE analysis of 3µg ab171474.
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